Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos

Jankovičová, Jana; Neuerová, Zdeňka; Sečová, Petra; Bartóková, Michaela; Bubeníčková, Filipa; Komrsková, Kateřina; Maňásková-Postlerová, Pavla; Antalíková, Jana

doi:10.1007/s00430-020-00676-0

Cited by 12 publications

(6 citation statements)

References 138 publications

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“…Among germ cells, oocytes are formed already during fetal life, while spermatozoa can be generated throughout adulthood. 138 Mature and developmentally competent oocytes are provided by the ovaries, which also can produce hormones to support the female phenotype and pregnancy. 139 In 2020, the first single-cell transcriptomic landscape of ovaries from young and aged nonhuman primates was presented.…”

Section: Applications Of Single-cell Sequencingmentioning

confidence: 99%

Single-cell technologies: From research to application

Wen

Huang

et al. 2022

The Innovation

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Section: Applications Of Single-cell Sequencingmentioning

confidence: 99%

Single-cell technologies: From research to application

Wen

Huang

et al. 2022

The Innovation

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“…65 Its expression in oocytes (and spermatozoa) is essential for the occurrence of sperm-egg fusion, a process involving the CD9-associated integrin a6b1 and the sperm ADAM2 (b-fertilin) among other players. [68][69][70][71][72][73][74] The role of integrin a6b1 was nonetheless challenged. 75 Thus, CD9-deficient oocytes do not fuse properly with sperm during fertilization and, as a consequence, CD9 knockout mice have reduced fertility.…”

Section: Cd9 In Tetraspanin Web and Plasma Membranementioning

confidence: 99%

CD9, a tetraspanin target for cancer therapy?

Lorico

Lorico-Rappa

Karbanová

et al. 2021

Exp Biol Med (Maywood)

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In the present minireview, we intend to provide a brief history of the field of CD9 involvement in oncogenesis and in the metastatic process of cancer, considering its potential value as a tumor-associated antigenic target. Over the years, CD9 has been identified as a favorable prognostic marker or predictor of metastatic potential depending on the cancer type. To understand its implications in cancer beside its use as an antigenic biomarker, it is essential to know its physiological functions, including its molecular partners in a given cell system. Moreover, the discovery that CD9 is one of the most specific and broadly expressed markers of extracellular membrane vesicles, nanometer-sized entities that are released into extracellular space and various physiological body fluids and play a role in intercellular communication under physiological and pathological conditions, notably the establishment of cancer metastases, has added a new dimension to our knowledge of CD9 function in cancer. Here, we will discuss these issues as well as the possible cancer therapeutic implications of CD9, their limitations, and pitfalls.

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“…Another family of candidate proteins with a role in sperm—egg interaction is that of the transmembrane tetraspanins. In mammals, the tetraspanin family members participate in primary sperm binding, gamete fusion, and polyspermy blocking ( Jankovicova et al, 2020 ). Of the tetraspanins, the cluster of differentiation (CD9) is essential in the mouse gamete fusion and fertilization ( Kaji et al, 2000 ; Miyado et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Positive selection on ADAM10 builds species recognition in the synchronous spawning coral Acropora

Morita

Kitanobo

Ohki

et al. 2023

Front. Cell Dev. Biol.

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The reef-building coral Acropora is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of Acropora digitifera using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of A. digitifera that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of A. digitifera, and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only A. digitifera fertilization but not the relative synchronous spawning species A. papillare. This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.

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Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos

Cited by 12 publications

References 138 publications

Single-cell technologies: From research to application

Single-cell technologies: From research to application

CD9, a tetraspanin target for cancer therapy?

Positive selection on ADAM10 builds species recognition in the synchronous spawning coral Acropora

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