Tetrazolium Test to Evaluate &amp;lt;i&amp;gt;Stizolobium aterrimum&amp;lt;/i&amp;gt;  Seeds Quality

Deminicis, Bruno Borges; Rodrigues, Patricia Do R.; Faria, Bráulio P.; Vieira, Henrique Duarte; Filho, Ant^onio D. Pandolfi; Freitas, Guilherme Santos

doi:10.4236/ajps.2014.51019

Cited by 5 publications

(5 citation statements)

References 4 publications

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“…parahyba (Fogaça et al, 2011), Annona cherimola Mill. x Annona squamosa L. (Gimenez et al, 2014), Stizolobium aterrimum (Deminicis et al, 2014) y Crambe marítima L. (Guimarães et al, 2015). Lo antes anotado, se ratificó con los resultados obtenidos en la prueba de germinación en cámara germinativa, cuyo porcentaje de plántulas germinadas (85% -95%), no presentó diferencias significativas con los porcentajes de semillas viables estimados mediante los diferentes tratamientos de la prueba bioquímica de tetrazolio (Cuadro 2).…”

Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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© 2017 Agronomía Mesoamericana es desarrollada en la Universidad de Costa Rica y se encuentra licenciada con Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Costa Rica. Para más información escríbanos a pccmca@ucr.ac.cr ResumenEl objetivo del estudio fue describir las características morfométricas, anatómicas y la viabilidad de las semillas de Cedrela odorata y Cariniana pyriformis. El trabajo se llevó a cabo en la Universidad de Córdoba de Montería, Colombia, en el período comprendido entre abril de 2012 a julio de 2014; con una metodología descriptiva de laboratorio, la prueba de tetrazolio y la prueba de germinación en cámara germinativa. Se utilizaron semillas de lotes comerciales de tres localidades del departamento de Córdoba. Se seleccionaron al azar cinco árboles de cada especie y se tomaron al azar cinco muestras de cien semillas de cada uno. La descripción de la anatomía se hizo con base en diez semillas de cada especie. Para la prueba de tetrazolio, se estableció un experimento bajo un diseño completamente al azar, con seis tratamientos y cuatro repeticiones de veinticinco semillas. Los seis tratamientos se originaron al combinar las concentraciones de tetrazolio de 0,5, 1,0 y 1,5%, con dos y tres horas de inmersión en la solución. Las características externas de C. odorata presentaron menor variación que las de C. pyriformis, especialmente aquellas relacionadas con el peso. Cuatro patrones topológicos fueron identificados en las semillas de cada especie. Se pudo determinar la viabilidad para C. pyriformis y C. odorata, con inmersiones por dos horas en concentraciones de 0,5 y 1,0% de tetrazolio, respectivamente.Palabras clave: calidad de semilla, germinación de semillas, prueba de tetrazolio. AbstractThe aim of the study was to identify morphometric, anatomical, and the viability characteristics of the seeds of Cedrela odorata and Cariniana pyriformis. The study was carried out at the Universidad de Córdoba of Montería, Colombia, from April 2012 to July 2014; the study was carried out with a laboratory descriptive methodology, tetrazolium and germination tests in germination chamber were performed. Seeds of commercial lots from three locations in the department of Cordoba, were used. Five trees of each specie were randomly selected and from each tree, five samples of one hundred seeds, were randomly selected. The description of the shape and the anatomy of the seeds was made according to ten seeds of each specie. For the tetrazolium tests, a completely randomized design experiment, with six treatments, and four replications of 25 seeds, was established. The six treatments were originated

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Section: Evaluación De Diferentes Concentraciones De Tetrazolio Y Tieunclassified

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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“…Temperatures of 35 and 40 ºC were used in the present study in order to decrease the duration of the test; however, the use of higher temperatures did not allow the utilization of tetrazolium solution at 1% in the present study, for promoting intense colorations in the entire extension of the embryo, thus denoting the non-viability of the seeds. Different results were obtained by Deminics et al (2014), who observed that the concentration of the tetrazolium solution at 1.0% for 150 min allowed to evaluate the viability of the lots of velvet bean seeds even at low temperatures, which can be related to some intrinsic characteristic of the species.…”

Section: Resultsmentioning

confidence: 89%

Tetrazolium test for the viability of gherkin seeds

Paiva

Torres

Almeida

et al. 2017

Revista Ciência Agronômica

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The tetrazolium test is a quick and effective method for evaluating viability in seeds. The aim of this study was to determine a methodology for using the tetrazolium test, and its efficiency in verifying viability in seeds of the gherkin (Cucumis anguria L.). Consequently, five batches of gherkin seed were initially evaluated by the tests for germination, first count and speed of germination. The batches were then subjected to treatments in order to adjust the methodology for the tetrazolium test, evaluating three concentrations of tetrazolium solution (0.100, 0.075 and 0.050%), kept in the dark for two, four and six hours, under temperatures of 35 °C and 40 °C, with four replications of 25 seeds per treatment. At the end of the staining period, the seeds were washed in running water, split lengthways between the cotyledons, and classified as viable and non-viable. Evaluation of viability in seeds of the gherkin by tetrazolium test is efficient when carried out at a concentration of 0.050%, either for six hours at 35 °C or four hours at 40 °C.

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“…Where, SP= Staining Percent, RSS=Red Stained Seeds, TSS= Total Stained Seeds. (Deminicis et al, 2014;Senoz, 2012;Patil and Dadlani, 2009;Leadem, 1984;Copeland and McDonald, 2001 and McDonald and Wilson, 1979) [14,51,41,27,11,32]…”

Section: Sp= [Rss/tss] X 100mentioning

confidence: 99%

Seed Germiability, viability and longevity of Rhododendron arboreum var. arboreum Sm.

Kumar¹,

Sharma²

2020

Int. J. Chem. Stud.

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The present study has been undertaken with the objective to assess the germiability, viability and longevity of R. arboreum seeds at laboratory conditions as these factors are very important to promote the process of natural regeneration of species in wild which ultimately responsible for the better survival and existence of species. The seeds for experiment were collected from Craignaino forest in Mashobara Range of Shimla forest division. Seed parameters like Germination per cent, germination energy and germination value were calculated to test the germiability from the freshly collected seeds continuously for the period of six years. Seed viability was assessed through germination method and TTZ (Tetrazolium salt) method. A storage trial was designed by using polythene bags and cloth bags as a medium of storage of seeds, to check the longevity of the seeds under three different temperature gradients (deep freezer, Multi cooling chamber and normal room temperature). The results are diverse, having common observation with higher viability in freshly collected seeds as compared to the previous years. The overall Results showed tendency of decline in germiability as well as loss in viability over a period of time. The deep freeze (-20°C) is the best suitable temperature gradient for the storage of R. arboreum seeds as the maximum viability of the seeds has been observed in this medium with the 50% decline in viability in the period of 3 years.

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Tetrazolium Test to Evaluate <i>Stizolobium aterrimum</i> Seeds Quality

Cited by 5 publications

References 4 publications

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Tetrazolium test for the viability of gherkin seeds

Seed Germiability, viability and longevity of Rhododendron arboreum var. arboreum Sm.

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