Textile-templated electrospun anisotropic scaffolds for regenerative cardiac tissue engineering

Perets, Anat; Ayaz, Hasan; Gilroy, Kyle D.; Govindaraj, M.; Brookstein, David; Lelkes, Peter I.

doi:10.1016/j.biomaterials.2014.06.029

Cited by 84 publications

(22 citation statements)

References 62 publications

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“…Scanning area is 50 × 50 μm. Reprinted from [212] with permission from Elsevier; (iv) Valve interstitial cells infiltrating electrospun scaffolds (SEM colored). Reprinted from [217] with permission from Elsevier.…”

Section: Figmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

115

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Extracellular matrix (ECM) structure and biochemistry provide cell-instructive cues that promote and regulate tissue growth, function, and repair. From a structural perspective, the ECM is a scaffold that guides the self-assembly of cells into distinct functional tissues. The ECM promotes the interaction between individual cells and between different cell types, and increases the strength and resilience of the tissue in mechanically dynamic environments. From a biochemical perspective, factors regulating cell-ECM adhesion have been described and diverse aspects of cell-ECM interactions in health and disease continue to be clarified. Natural ECMs therefore provide excellent design rules for tissue engineering scaffolds. The design of regenerative three-dimensional (3D) engineered scaffolds is informed by the target ECM structure, chemistry, and mechanics, to encourage cell infiltration and tissue genesis. This can be achieved using nanofibrous scaffolds composed of polymers that simultaneously recapitulate 3D ECM architecture, high-fidelity nanoscale topography, and bio-activity. Their high porosity, structural anisotropy, and bio-activity present unique advantages for engineering 3D anisotropic tissues. Here, we use the heart as a case study and examine the potential of ECM-inspired nanofibrous scaffolds for cardiac tissue engineering. We asked: Do we know enough to build a heart? To answer this question, we tabulated structural and functional properties of myocardial and valvular tissues for use as design criteria, reviewed nanofiber manufacturing platforms and assessed their capabilities to produce scaffolds that meet our design criteria. Our knowledge of the anatomy and physiology of the heart, as well as our ability to create synthetic ECM scaffolds have advanced to the point that valve replacement with nanofibrous scaffolds may be achieved in the short term, while myocardial repair requires further study in vitro and in vivo.

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Section: Figmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

115

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“…[ 11 ] Evidence has increasingly suggested that such anisotropic fi ber organizations induce cell elongation, promote cell migration, and regulate cell differentiation via integrin-mediated outside-in signaling. [ 12 ] Building various anisotropic features into electrospun matrices [13][14][15][16] is highly desirable because of its potential to better mimic the unique mechanical and biologic functions of tissue ECM and consequently guide preferred tissue formation. However, current electric fi eld intervention tactics can only modulate the fi ber arrangement without feasibility of accommodating many other hierarchical and complex characteristics of native tissues (e.g., various patterned organization of cells).…”

mentioning

confidence: 99%

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Jia

Lamarre

et al. 2014

Advanced Materials

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With the assistance of an ink-jet printer, solvent (the "ink") can be controllably and reproducibly printed onto electrospun nanofiber meshes (the "paper") to generate various micropatterns and subsequently guide distinct cellular organization and phenotype expression. In combination with the nanofiber-assisted layer-by-layer cell assembly, the patterned electrospun meshes will define an instructive microenvironment for guided tissue formation.

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“…In these polymeric fibers, primary cardiomyocytes developed mature contractile machinery (sarcomeres) and showed electrical activity using voltage-sensitive dyes over 7 days. Similar cardiomyocyte contractility and survival have been observed with other biodegradable polymers used in electrospinning such as polyurethane (158), poly(lactic-co-glycolic) acid (PLGA) (159), and polycaprolactone (PCL) (160). However, these in vitro studies were carried out in the absence of fibroblasts which can modulate cardiomyocyte function and structure (161) and thereby impact long term survival and tissue integration.…”

Section: Electrospinning Nanofibersmentioning

confidence: 64%

Tissue Engineering Strategies for Myocardial Regeneration: Acellular Versus Cellular Scaffolds?

Domenech

Polo-Corrales

Ramı́rez-Vick

et al. 2016

Tissue Engineering Part B: Reviews

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Heart disease remains one of the leading causes of death in industrialized nations with myocardial infarction (MI) contributing to at least one fifth of the reported deaths. The hypoxic environment eventually leads to cellular death and scar tissue formation. The scar tissue that forms is not mechanically functional and often leads to myocardial remodeling and eventual heart failure. Tissue engineering and regenerative medicine principles provide an alternative approach to restoring myocardial function by designing constructs that will restore the mechanical function of the heart. In this review, we will describe the cellular events that take place after an MI and describe current treatments. We will also describe how biomaterials, alone or in combination with a cellular component, have been used to engineer suitable myocardium replacement constructs and how new advanced culture systems will be required to achieve clinical success.

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Textile-templated electrospun anisotropic scaffolds for regenerative cardiac tissue engineering

Cited by 84 publications

References 62 publications

Fibrous scaffolds for building hearts and heart parts

Fibrous scaffolds for building hearts and heart parts

Patterned Electrospun Nanofiber Matrices Via Localized Dissolution: Potential for Guided Tissue Formation

Tissue Engineering Strategies for Myocardial Regeneration: Acellular Versus Cellular Scaffolds?

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