2005
DOI: 10.1534/genetics.105.046557
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TFIID and Spt-Ada-Gcn5-Acetyltransferase Functions Probed by Genome-wide Synthetic Genetic Array Analysis Using a Saccharomyces cerevisiae taf9-ts Allele

Abstract: TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. The essentiality of the TAFs has made it difficult to ascertain their roles in TFIID and SAGA function. Here we performed a genomic synthetic genetic array analysis using a temperature-sensitive allele of TAF9 as a query. Results from this experiment showed that TAF9 interacts genetically with: (1) genes for multiple transcription factor complexes predomin… Show more

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Cited by 18 publications
(23 citation statements)
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“…The genotypes of yeast strains used in this study are listed in Table 1 (Wolfger et al 1997;Gao et al 2004) and described previously for deletion strains derived from BY4741 (Milgrom et al 2005). The null alleles introduced into pdr1-3 were carried out by PCRmediated allele transfer from deletion alleles of nonessential genes available from the collection of synthetic genetic arrays (Tong et al 2001) or by PCR-based gene deletion using modification cassettes as previously described (Longtine et al 1998).…”
Section: Methodsmentioning
confidence: 99%
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“…The genotypes of yeast strains used in this study are listed in Table 1 (Wolfger et al 1997;Gao et al 2004) and described previously for deletion strains derived from BY4741 (Milgrom et al 2005). The null alleles introduced into pdr1-3 were carried out by PCRmediated allele transfer from deletion alleles of nonessential genes available from the collection of synthetic genetic arrays (Tong et al 2001) or by PCR-based gene deletion using modification cassettes as previously described (Longtine et al 1998).…”
Section: Methodsmentioning
confidence: 99%
“…was induced by treatment of cells for 45 min in YPD medium containing 0.2 mg/ml (0.71 mm) cycloheximide (CYH) as described (Gao et al 2004;Milgrom et al 2005).…”
Section: Wcs265 (Fy1679-28c)mentioning
confidence: 99%
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