TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in <i>Arabidopsis thaliana</i>

Li, Ning; Muthreich, Martin; Huang, Lijun; Thurow, Corinna; Sun, Tongjun; Zhang, Yuelin; Gatz, Christiane

doi:10.1111/nph.15496

Cited by 49 publications

(63 citation statements)

References 48 publications

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“…Protoplast assays were carried out as described previously by Yoo et al [30] and Li et al [37], using four-week-old soil-grown Arabidopsis thaliana wild-type and mutant plants. All chemicals used in this study were obtained from Sigma-Aldrich (Darmstadt, Germany), unless otherwise stated.…”

Section: Protoplasts Preparation and Transfectionmentioning

confidence: 99%

Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts

Uhrig

Thurow

et al. 2019

Cells

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The phytohormone jasmonic acid (JA) plays an important role in various plant developmental processes and environmental adaptations. The JA signaling pathway has been well-elucidated in the reference plant Arabidopsis thaliana. It starts with the perception of the active JA derivative, jasmonoyl-isoleucine (JA-Ile), by the F-box protein COI1 which is part of the E3-ligase SCFCOI1. Binding of JA-Ile enables the interaction between COI1 and JAZ repressor proteins. Subsequent degradation of JAZ proteins leads to the activation of transcription factors like e.g., MYC2. Here we demonstrate that the pathway can be reconstituted in transiently transformed protoplasts. Analysis of the stability of a JAZ1-fLuc fusion protein as a function of COI1 transiently expressed in coi1 protoplasts allows structure function analysis of both JAZs and COI1. Using this system, we found that conserved cysteines in COI1 influence steady state COI1 protein levels. Using a luciferase reporter gene under the control of the JAZ1 promoter enable to address those features of JAZ1 that are required for MYC2 repression. Interestingly, the conserved TIFY-motif previously described to interact with NINJA to recruit the corepressor TOPLESS is not necessary for repression. This result is in favor of the alternative repression mode that proposes a direct competition between repressive JAZs and promotive MEDIATOR25 at MYC2. Finally, using protoplasts from the aos coi1 double mutant, which is deficient in JA synthesis and perception, we provide a system that has the potential to study the activity of different COI1 variants in the presence of different ligands.

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Section: Protoplasts Preparation and Transfectionmentioning

confidence: 99%

Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts

Uhrig

Thurow

et al. 2019

Cells

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“…However, a cassava CC-type GRX MeGRXC15 interacted with TGA5, function as a positive regulator of several stress-related transcription factors in transgenic Arabidopsis [ 8 ]. ROXY8 and ROXY9 were identified as a regulator in hyponastic growth of Arabidopsis by negatively modifying TGA1 and TGA4 [ 23 ]. GRXS25 could trigger metabolism of pesticide residue in tomato plants through activating TGA2 factor by posttranslational redox modification [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, another CC-type GRX namely PHS9 regulated seed germination of rice through the integration of ROS signaling and ABA signaling [ 26 ]. ROXY8 , ROXY9 , and ROXY19/GRX480 involve in auxin pathway by regulating auxin-induced and growth-related genes therefore affect hyponastic growth of Arabidopsis [ 23 ]. Recently, a tomato CC-type GRX GRXS25 was identified as a regulator in brassinosteroid (BR) pathway [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Role of cassava CC-type glutaredoxin MeGRXC3 in regulating sensitivity to mannitol-induced osmotic stress dependent on its nuclear activity

Ruan

Guo

et al. 2022

BMC Plant Biol

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Background We previously identified six drought-inducible CC-type glutaredoxins in cassava cultivars, however, less is known about their potential role in the molecular mechanism by which cassava adapted to abiotic stress. Results Herein, we investigate one of cassava drought-responsive CC-type glutaredoxins, namely MeGRXC3, that involved in regulation of mannitol-induced inhibition on seed germination and seedling growth in transgenic Arabidopsis. MeGRXC3 overexpression up-regulates several stress-related transcription factor genes, such as PDF1.2, ERF6, ORA59, DREB2A, WRKY40, and WRKY53 in Arabidopsis. Protein interaction assays show that MeGRXC3 interacts with Arabidopsis TGA2 and TGA5 in the nucleus. Eliminated nuclear localization of MeGRXC3 failed to result mannitol-induced inhibition of seed germination and seedling growth in transgenic Arabidopsis. Mutation analysis of MeGRXC3 indicates the importance of conserved motifs for its transactivation activity in yeast. Additionally, these motifs are also indispensable for its functionality in regulating mannitol-induced inhibition of seed germination and enhancement of the stress-related transcription factors in transgenic Arabidopsis. Conclusions MeGRXC3 overexpression confers mannitol sensitivity in transgenic Arabidopsis possibly through interaction with TGA2/5 in the nucleus, and nuclear activity of MeGRXC3 is required for its function.

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“…Protoplast transfection and dual luciferase assay were performed as described previously in [43,51]. Briefly, 5 µg of protein-fusion reporter plasmids or promoter-driven reporter plasmids and 5 µg of effector plasmids were co-transfected.…”

Section: Protoplast Transfection and Dual Luciferase Report Assaymentioning

confidence: 99%

Molecular Rewiring of the Jasmonate Signaling Pathway to Control Auxin-Responsive Gene Expression

Cao

Miu

et al. 2020

Cells

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The plant hormone jasmonic acid (JA) has an important role in many aspects of plant defense response and developmental process. JA triggers interaction between the F-box protein COI1 and the transcriptional repressors of the JAZ family that leads the later to proteasomal degradation. The Jas-motif of JAZs is critical for mediating the COI1 and JAZs interaction in the presence of JA. Here, by using the protoplast transient gene expression system we reported that the Jas-motif of JAZ1 was necessary and sufficient to target a foreign reporter protein for COI1-facilitated degradation. We fused the Jas-motif to the SHY2 transcriptional repressor of auxin signaling pathway to create a chimeric protein JaSHY. Interestingly, JaSHY retained the transcriptional repressor function while become degradable by the JA coreceptor COI1 in a JA-dependent fashion. Moreover, the JA-induced and COI1-facilitated degradation of JaSHY led to activation of a synthetic auxin-responsive promoter activity. These results showed that the modular components of JA signal transduction pathway can be artificially redirected to regulate auxin signaling pathway and control auxin-responsive gene expression. Our work provides a general strategy for using synthetic biology approaches to explore and design cell signaling networks to generate new cellular functions in plant systems.

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TGACG‐BINDING FACTORs (TGAs) and TGA‐interacting CC‐type glutaredoxins modulate hyponastic growth in Arabidopsis thaliana

Cited by 49 publications

References 48 publications

Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts

Reconstitution of the Jasmonate Signaling Pathway in Plant Protoplasts

Role of cassava CC-type glutaredoxin MeGRXC3 in regulating sensitivity to mannitol-induced osmotic stress dependent on its nuclear activity

Molecular Rewiring of the Jasmonate Signaling Pathway to Control Auxin-Responsive Gene Expression

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