TGF-alpha, EGF, and their cognate EGF receptor are co-expressed with desmin during embryonic, fetal, and neonatal myogenesis in mouse tongue development

Yamane, Akira; Mayo, Mark L.; Bringas, Pablo; Chen, Lynn; Huynh, Manghi; Thai, Khai; Shum, Lillian; Slavkin, Harold C.

doi:10.1002/(sici)1097-0177(199708)209:4<353::aid-aja3>3.0.co;2-h

Cited by 21 publications

(13 citation statements)

References 82 publications

(68 reference statements)

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“…Moreover, the expression of desmin at E12 during which separation of the tongue and mandible was noted indicates that desmin is has a functional at this stage. This coincides with previous studies that vimentin and desmin are involved in the growth of skeletal muscles 7,[12][13][14]16) . Separation of the tongue from the mandible started at E12 although it was still incomplete at this point.…”

Section: Discussionsupporting

confidence: 93%

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Expression of Intermediate Filaments in the Development of Genioglossus Muscle

Kikuchi

Kishi

Yamamoto

et al. 2012

J. Hard Tissue Biology.

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： In recent years, the expression of desmin intermediate filament in muscle and tendon attachment brought about mechanical stress has been reported. Mastication and swallowing exercises can stimulate the growth of the tongue through functional load. However, the expression of intermediate filaments during development has not been investigated. Moreover, the relevance of the surrounding tissues to the development of the tongue is still unknown. Therefore, the expression of desmin in embryonic mouse tongue during development and the expression of vimentin in mesenchymal tissues were investigated by immunohistochemistry and Western Blot. Results revealed that at E11, there was no distinction between the tongue and mandible. Vimentin expression was detected in the entire tissue at E11 and the expression spread to the surrounding tissues over time. Desmin was not detected at E11 however, at E12, desmin expression was observed at the attachment of genioglossus and intrinsic muscle of the tongue which then became more intense and spread throughout the surrounding tissues. The IHC results coincided with Western Blot analysis. The results suggest that desmin expression at the site of intense mechanical loading plays an important function in mastication and swallowing.

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Section: Discussionsupporting

confidence: 93%

“…Furthermore, vimentin is also known as a marker of mesenchymal cells differentiating into various types of cells 12) . Furthermore, vimentin and desmin are deeply involved in the growth of skeletal muscle 13,14) . The expression of vimentin and desmin in muscle tissue has been investigated so far [15][16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Expression of Intermediate Filaments in the Development of Genioglossus Muscle

Kikuchi

Kishi

Yamamoto

et al. 2012

J. Hard Tissue Biology.

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“…We previously reported several different functions of peptide growth factors in the development of tongue muscle cells from limb and cultured myogenic cell lines: TGFα promotes early differentiation of mouse tongue myoblasts [33-35], whereas it does not affect the differentiation of the C2C12 myoblast [36]; and the signal of TGFβ3, but not of 1 or 2, plays a role in the early stages in the differentiation of mouse tongue muscle cells through TGFβ receptor I, TGFβ receptor II, and smad2/3 [37], but TGFβ1 inhibits the differentiation of limb skeletal muscle cells [38,39]. In addition to the function of growth factors, there are several studies showing other differences between tongue muscle cells, and limb and cultured myogenic cell lines, such that fMyHC is expressed not only in the myotubes and myofibers of tongue muscles but also in the myoblasts of tongue muscles, although it is not expressed in the myoblasts of trunk and limb muscles [40].…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein-2 functions as a negative regulator in the differentiation of myoblasts, but not as an inducer for the formations of cartilage and bone in mouse embryonic tongue

et al. 2011

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BackgroundIn vitro studies using the myogenic cell line C2C12 demonstrate that bone morphogenetic protein-2 (BMP-2) converts the developmental pathway of C2C12 from a myogenic cell lineage to an osteoblastic cell lineage. Further, in vivo studies using null mutation mice demonstrate that BMPs inhibit the specification of the developmental fate of myogenic progenitor cells. However, the roles of BMPs in the phases of differentiation and maturation in skeletal muscles have yet to be determined. The present study attempts to define the function of BMP-2 in the final stage of differentiation of mouse tongue myoblast.ResultsRecombinant BMP-2 inhibited the expressions of markers for the differentiation of skeletal muscle cells, such as myogenin, muscle creatine kinase (MCK), and fast myosin heavy chain (fMyHC), whereas BMP-2 siRNA stimulated such markers. Neither the recombinant BMP-2 nor BMP-2 siRNA altered the expressions of markers for the formation of cartilage and bone, such as osteocalcin, alkaline phosphatase (ALP), collagen II, and collagen X. Further, no formation of cartilage and bone was observed in the recombinant BMP-2-treated tongues based on Alizarin red and Alcian blue stainings. Neither recombinant BMP-2 nor BMP-2 siRNA affected the expression of inhibitor of DNA binding/differentiation 1 (Id1). The ratios of chondrogenic and osteogenic markers relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH, a house keeping gene) were approximately 1000-fold lower than those of myogenic markers in the cultured tongue.ConclusionsBMP-2 functions as a negative regulator for the final differentiation of tongue myoblasts, but not as an inducer for the formation of cartilage and bone in cultured tongue, probably because the genes related to myogenesis are in an activation mode, while the genes related to chondrogenesis and osteogenesis are in a silencing mode.

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“…IGF-I promotes the differentiation of tongue myoblasts (Yamane et al 2002a,b), whereas HGF plays essential roles in the migration and proliferation of tongue myogenic cells (Amano et al 2002) and inhibits the differentiation of tongue myoblasts ). TGFα does not play an essential role in the proliferation of tongue myogenic cells, although it promotes the early differentiation of tongue myoblasts (Yamane et al 1997(Yamane et al , 1998a. The roles of IGF-I, HGF, and TGFα in tongue myogenesis are almost identical to those in the myogenesis of limb and cultured myogenic cell lines, such as C2 and L6, suggesting that the program that governs myogenesis is similar in all these cell types.…”

Section: Developmental Program Governing Tongue Myogenesismentioning

confidence: 99%

Embryonic and postnatal development of masticatory and tongue muscles

Yamane

2005

Cell Tissue Res

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This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other muscles, such as the trunk (deep muscles of the back, body wall muscles) and limb muscles, originate from the somites. The program controlling the early stages of masticatory myogenesis, such as the specification and migration of muscle progenitor cells, is distinctly different from those in trunk and limb myogenesis. Tongue myogenesis follows a similar regulatory program to that for limb myogenesis. Myogenesis and synaptogenesis in the masticatory muscles are delayed in comparison with other muscles and are not complete even at birth, whereas the development of tongue muscles proceeds faster than those of other muscles and ends at around birth. The regulatory programs for masticatory and tongue myogenesis seem to depend on the developmental origins of the muscles, i.e., the origin being either a somite or somitomere, whereas myogenesis and synaptogenesis seem to progress to serve the functional requirements of the masticatory and tongue muscles.

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TGF-alpha, EGF, and their cognate EGF receptor are co-expressed with desmin during embryonic, fetal, and neonatal myogenesis in mouse tongue development

Cited by 21 publications

References 82 publications

Expression of Intermediate Filaments in the Development of Genioglossus Muscle

Expression of Intermediate Filaments in the Development of Genioglossus Muscle

Bone morphogenetic protein-2 functions as a negative regulator in the differentiation of myoblasts, but not as an inducer for the formations of cartilage and bone in mouse embryonic tongue

Embryonic and postnatal development of masticatory and tongue muscles

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