TGF-β Activity of a Demineralized Bone Matrix

Panahipour, Layla; Omerbasic, Anes; Nasirzade, Jila; Gruber, Reinhard

doi:10.3390/ijms22020664

Cited by 13 publications

(14 citation statements)

References 42 publications

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“…Fibrin 61 , vitronectin and fibronectin 62 can adsorb TGF-β and work as a scaffold for the growth factors released from the dentin and other sources applied in a clinical setting. For example, platelet-rich fibrin 63 , demineralized bone grafts 64 and even certain collagen-based biomaterials 65 release a TGF-β activity. To come closer to an answer, we have recently evaluated the impact of ADL bound to a collagen membrane on in vivo bone formation in a rat calvaria defect model.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

Nasirzade

Kargarpour

Mitulović

et al. 2021

Sci Rep

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Particulate autologous tooth roots are increasingly used for alveolar bone augmentation; however, the proteomic profile of acid dentin lysate and the respective cellular response have not been investigated. Here we show that TGF-β1 is among the 226 proteins of acid dentin lysate (ADL) prepared from porcine teeth. RNA sequencing identified 231 strongly regulated genes when gingival fibroblasts were exposed to ADL. Out of these genes, about one third required activation of the TGF-β receptor type I kinase including interleukin 11 (IL11) and NADPH oxidase 4 (NOX4). Reverse transcription-quantitative polymerase chain reaction and immunoassay confirmed the TGF-β-dependent expression of IL11 and NOX4. The activation of canonical TGF-β signaling by ADL was further confirmed by the phosphorylation of Smad3 and translocation of Smad2/3, using Western blot and immunofluorescence staining, respectively. Finally, we showed that TGF-β activity released from dentin by acid lysis adsorbs to titanium and collagen membranes. These findings suggest that dentin particles are a rich source of TGF-β causing a major response of gingival fibroblasts.

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Section: Discussionmentioning

confidence: 99%

Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

Nasirzade

Kargarpour

Mitulović

et al. 2021

Sci Rep

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“…Moreover, demineralized teeth are proposed as grafts for bone reconstruction [17,29]. DBM research is closely related to the discovery of the BMPs [1,7,30], yet attempts to identify other growth factors that survive the demineralization process are minor [13,31,32]. Surprisingly, however, even though the early stages of graft consolidation occur in a catabolic inflammatory environment [15], we lack research on whether DBM might influence the process of inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…Cells were preexposed to 25% acid lysates from DGC and MDF for 30 min before being exposed to 100 ng/mL LPS (Escherichia coli 0111: B41; Sigma Aldrich) for 24 h [18]. To test for the involvement of TGF-β signaling, the TGF-β receptor I kinase inhibitor SB431542 (Calbiochem, Merck, Billerica, MA, USA) was added at 10 µM [13]. In another set of experiments, RAW 264.7 cells were exposed to 10-300 mM NaCl prior to the LPS challenge.…”

Section: Murine Raw 2647 and Bone Marrow-derived Macrophagesmentioning

confidence: 99%

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Anti-Inflammatory Activity of a Demineralized Bone Matrix: An In Vitro Pilot Study

et al. 2022

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Demineralized bone matrix (DBM) is commonly used for the reconstruction of bone defects. Early graft consolidation involves a transient inflammatory process. It is, however, unclear whether DBM can modulate this process. To test this possibility, we prepared acid lysates of demineralized ground cortical (DGC) and moldable demineralized fibers (MDF). Murine RAW 264.7 and primary bone marrow macrophages were exposed to acid lysates of DGC and MFD prior to provoking an inflammatory response with lipopolysaccharide (LPS). Similarly, murine ST2 mesenchymal cells were exposed to DGC and MFD with and without interleukin 1β (IL1) and TNFα. We show here that acid lysates of DGC and MFD reduced the expression of IL1 and IL6 in RAW 264.7 macrophages, as determined by RT-PCR and, for IL6, by immunoassay. This response was confirmed with primary macrophages. Likewise, desalted acid lysates exert anti-inflammatory properties on RAW 264.7 cells and in ST2 cells, the forced expression of IL6, inducible nitric oxide synthase (iNOS) and chemokine ligand 5 (CCL5) was reduced. These in vitro findings suggest that DGC and MFD lower the inflammation-induced expression of inflammatory mediators in murine cell-based bioassays.

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“…In the late stage of chondrogenesis, the expression of TGF- β decreases, and the synthesis of some degradation enzyme inhibitors is induced to maintain the chondrocyte phenotype to ensure the stability and function of articular cartilage, which plays an important role in maintaining the integrity of articular cartilage. In addition, TGF- β can effectively regulate the processes of bone resorption and bone remodeling and also has a great regulatory effect on osteoblast replication and bone matrix synthesis [ 31 , 32 ]. TGF- β is an important factor in the process of bone formation and absorption, which can stimulate the synthesis of collagen by osteoblasts and osteoblasts, increase the synthesis of cartilage-specific type I collagen and fibronectin, and reduce the synthesis of type II and type X collagen.…”

Section: Bone Growth Factorsmentioning

confidence: 99%

Progress of Wnt Signaling Pathway in Osteoporosis

Gao

Chen

et al. 2023

Biomolecules

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Osteoporosis, one of the serious health diseases, involves bone mass loss, bone density diminishing, and degeneration of bone microstructure, which is accompanied by a tendency toward bone fragility and a predisposition to fracture. More than 200 million people worldwide suffer from osteoporosis, and the cost of treating osteoporotic fractures is expected to reach at least $25 billion by 2025. The generation and development of osteoporosis are regulated by genetic factors and regulatory factors such as TGF-β, BMP, and FGF through multiple pathways, including the Wnt signaling pathway, the Notch signaling pathway, and the MAPK signaling pathway. Among them, the Wnt signaling pathway is one of the most important pathways. It is not only involved in bone development and metabolism but also in the differentiation and proliferation of chondrocytes, mesenchymal stem cells, osteoclasts, and osteoblasts. Dkk-1 and SOST are Wnt inhibitory proteins that can inhibit the activation of the canonical Wnt signaling pathway and block the proliferation and differentiation of osteoblasts. Therefore, they may serve as potential targets for the treatment of osteoporosis. In this review, we analyzed the mechanisms of Wnt proteins, β-catenin, and signaling molecules in the process of signal transduction and summarized the relationship between the Wnt signaling pathway and bone-related cells. We hope to attract attention to the role of the Wnt signaling pathway in osteoporosis and offer new perspectives and approaches to making a diagnosis and giving treatment for osteoporosis.

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TGF-β Activity of a Demineralized Bone Matrix

Cited by 13 publications

References 42 publications

Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

Anti-Inflammatory Activity of a Demineralized Bone Matrix: An In Vitro Pilot Study

Progress of Wnt Signaling Pathway in Osteoporosis

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