TGF-β receptor type II and fetuin in the developing sheep neocortex

Dzięgielewska, Katarzyna M.; Williams, R.; Knott, Graham; Kitchener, PD; Monk, S. E.; Potter, Ann; Saunders, NR

doi:10.1007/s004410050958

Cited by 9 publications

(11 citation statements)

References 28 publications

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“…The morphology of those subsets of cells was different, but both cell types stained for IL-1β and only the cells of the white matter stained weakly for TNF-α. This pattern of staining was reminiscent of the distribution described for TGF-β receptor type II (Dziegielewska et al 1997). These cells stained with GSA lectin, a marker for microglia/monocytes (Boya et al 1991).…”

Section: Discussionmentioning

confidence: 67%

Acute-phase cytokines IL-1β and TNF-α in brain development

Dziegielewska¹,

Møller²,

Potter³

et al. 2000

Cell and Tissue Research

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The nervous and the immune systems share several molecules that control their development and function. We studied the temporal and spatial distribution of the immunoreactivity of two acute-phase cytokines, TNF-α and IL-1β, in the developing sheep neocortex and compared it with the well-described distribution of fetuin, a fetal glycoprotein also known to modulate the production of cytokines by lipopolysaccharide (LPS)-stimulated monocytes and macrophages. TNF-α was present first at embryonic day 30 (E30) (term is 150 days in sheep) as a faint band of immunoreactivity between the ventricular zone and the primordial plexiform layer (preplate). IL-1β was detected at the first appearance of the cortical plate (E35-E40). Both cytokines were present on both sides of the cortical plate, which contained fetuin-positive cells, but was free from cytokine staining. By E60, TNF-α immunoreactivity was less prominent than that of IL-1β and was confined to the marginal zone and outer developing white matter; IL-1β was present in the marginal zone and in two bands of immunoreactive cells, one at the border of the cortical plate/developing layer VI (cells of neuronal morphology) and the other at the border of layer V and the developing white matter (identified as microglia). By E80, TNF-α staining had disappeared and IL-1β-immunopositive microglia were no longer detectable. By E100-E140 only a few immunoreactive cells were identified in layers V-VI; these did not co-localize with fetuin-positive cells. The differences in distribution between fetuin and the two cytokines suggest that the opsonizing role of fetuin, proposed for monocyte production of cytokines, is probably not present in the developing brain. However, early in neocortical development TNF-α and IL-1β were present in the subplate zone at a time of intense synaptogenesis.

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Section: Discussionmentioning

confidence: 67%

Acute-phase cytokines IL-1β and TNF-α in brain development

Dziegielewska¹,

Møller²,

Potter³

et al. 2000

Cell and Tissue Research

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“…Sections from fetal sheep (E28-E150; Dziegielewska et al 1997) postnatal (P0-P20) M. domestica (South American opossum; Knott et al 1997) and adult rat (Moos and Møllgård 1993) were available from previous studies. All animal experiments were performed according to NHMRC guidelines and were approved by the University of Melbourne Animal Experimentation Ethics Committee.…”

Section: Methodsmentioning

confidence: 99%

Aquaporin-1 in the choroid plexuses of developing mammalian brain

Johansson

Dziegielewska

et al. 2005

Cell Tissue Res

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The normal brain develops within a well-controlled stable internal "milieu" protected by specialised mechanisms referred to collectively as blood-brain barriers. A fundamental feature of this environment is the control of water flow in and out of the developing brain. Because of limited vascularisation of the immature brain, choroid plexuses, via the cerebrospinal fluid, have been proposed as the main route of fluid exchange between the blood and brain interfaces. We describe the temporal expression and appearance of aquaporin-1 (AQP1) which is important for water transfer across adult choroid plexuses. AQP1 expression was studied in rat embryos using real time reverse transcription/polymerase chain reaction. mRNA for AQP1 was present in rat brain at embryonic day 12 (E12) one day before the protein was detectable in the fourth ventricular choroid plexus (the first plexus to appear); its relative levels increased at E13-E14 when more AQP1-immunoreactive cells appeared in all plexuses. The presence of AQP1 was determined immunocytochemically in five different mammalian species (rat, mouse, human, sheep and opossum) in all four choroid plexuses from their earliest appearance. In all five species studied, the appearance of AQP1 immunoreactivity followed the same developmental sequence: the fourth, lateral and, finally, third ventricular choroid plexus. The stage of choroid plexus development when AQP1 was first detected in all five species and in all four choroid plexuses corresponded to the transition between Stages I and II. The cellular localisation of AQP1 in all choroid plexuses, as soon as it was detectable, had the characteristic apical membrane distribution previously described in the adult; a basolateral membrane localisation was also observed.

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“…Equivalent of fetuin in other mammalian species is alpha 2-HS glycoprotein (AHSG) [13]. Fetuin is thought to play an important role in fetal development [13,14] and neonatal skeletal development [15]. It is also important for postnatal bone growth, remodeling and contributes to insulin resistance during normal pregnancy and gestational diabetes [16].…”

Section: Introductionmentioning

confidence: 99%

“…It is also important for postnatal bone growth, remodeling and contributes to insulin resistance during normal pregnancy and gestational diabetes [16]. It has been shown that fetuin and various cytokines have a developmentally regulated appearance and expression in the developing neocortex [13,15]. A short 19-amino-acid sequence of the transforming growth factors beta *Address correspondence to this author at the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh -202002, India; Tel: 91-571-2720388; Fax: + 91-571-2721776; E-mails: rizwanhkhan@hotmail.com; rizwanhkhan1@yahoo.com (TGF-beta) type-II receptor and in vitro fetuin binds to members of the TGF-beta family of cytokines.…”

Section: Introductionmentioning

confidence: 99%