TGF-β1-Mediated FDNCR1 Regulates Porcine Preadipocyte Differentiation via the TGF-β Signaling Pathway

Abstract

Adipocyte differentiation and lipid metabolism have important regulatory effects on the quality of meat from livestock. A variety of transcription factors regulate preadipocyte differentiation. Several studies have revealed that transforming growth factor-beta (TGF-β1) may play a key role in epithelial–mesenchymal transition (EMT); however, little is known about the effects of TGF-β1 treatment on porcine preadipocytes. To explore the role of TGF-β1 in porcine adipocyte differentiation, porcine preadipocytes were treated with 10 ng/mL TGF-β1, and two libraries were constructed for RNA-seq. We chose an abundant and differentially expressed long noncoding RNA (lncRNA), which we named fat deposition-associated long noncoding RNA1 (FDNCR1), for further study. RT-qPCR was used to detect mRNA levels of genes related to adipocyte differentiation. Triglyceride assay kits were used to detect lipid droplet deposition. TGF-β1 significantly suppressed porcine preadipocyte differentiation. We identified 8158 lncRNAs in total and 39 differentially expressed lncRNAs. After transfection with FDNCR1 siRNA, the mRNA expression of aP2, C/EBPα, and PPARγ and triglyceride levels significantly increased. Transfection with FDNCR1 siRNA significantly decreased protein levels of p-Smad2/Smad2 and p-Smad3/Smad3. These results demonstrate that FDNCR1 suppresses porcine preadipocyte differentiation via the TGF-β signaling pathway.

Integrative Analysis of lncRNA-miRNA-mRNA Regulatory Network Reveals Candidate lncRNAs Responsible for Meat Quality in Goats

“…For example, the LncRNA GAS5 inhibits lipogenesis in 3T3-L1 cells through the miR-21a-5p / PTEN signaling pathway [44]. FDNCR1 affects porcine lipogenesis by competitively binding miR-204 to regulate the TGF-β pathway [45]. However, for some pathways identi ed here, their involvement in the goat preadipocyte differentiation process is being reported for the rst time.…”

Key circRNAs from goat: discovery, integrated regulatory network and their putative roles in the differentiation of intramuscular adipocytes

“…The GO enrichment result showed that the mRNAs we found were all participated in macromolecule metabolic process. Three pathways, PI3K-Akt signaling pathway, AMPK signaling pathway and TGF-beta signaling pathway, got our attentions in KEGG enrichment result, cause they all having essential roles during adipocyte differentiation [ 26 , 55 , 56 ]. Then we obtained 5 key mRNAs in the circRNA-miRNA-mRNA network, FOXO3 [ 57 ], PPP2CA [ 58 ], EEIF4E [ 59 ], CDK6 [ 60 ] and ACVR1 [ 61 ], and they all showed valuable functions in fat-related research.…”

Integrative Analysis of lncRNA-miRNA-mRNA Regulatory Network Reveals the Key lncRNAs Implicated Potentially in the Differentiation of Adipocyte in Goats

“…For example, the LncRNA GAS5 inhibits lipogenesis in 3T3-L1 cells through the miR-21a-5p/PTEN signaling pathway ( Liu et al, 2018 ). FDNCR1 affects porcine lipogenesis by competitively binding miR-204 to regulate the TGF-β pathway ( Zhang et al, 2020 ). However, for some pathways identified here, their involvement in the goat preadipocyte differentiation process is being reported for the first time.…”