TGN38 and Small GTP Binding Proteins are Part of a Macromolecular Complex in the Trans-Golgi Network

Crosby, J. R.; Jones, Steven G.; Ladinsky, Mark S.; Howell, Kathryn E.

doi:10.1007/978-3-662-02928-2_25

Cited by 1 publication

(2 citation statements)

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“…Electron m icroscopy of cryosections labeled with colloidal gold probes shows TGN38/41 in the frans-m ost cisternae and the TG N (Luzio et al, 1990;Ladinsky and H owell, 1992;Crosby et al, 1993); this is illustrated in Fig. 1 A.…”

Section: Steady-state D Istrib Utio N Of Tgn38/41 In Nrk Cellsmentioning

confidence: 88%

“…TGN38/41 is a type 1, transmembrane heterodimeric pro tein, which resides primarily in the trans-most Golgi cistemae and the trans-Golgi network (TGN) (Luzio et al, 1990;Jones et al, 1993;Crosby et al, 1993). Both sub units contain the same luminal and transmembrane domains, but differ in that TGN41 has an extended cyto plasmic domain of 23 amino acids .…”

Section: Introductionmentioning

confidence: 99%

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An electron microscopic study of TGN38/41 dynamics

Ladinsky

Howell

1993

Journal of Cell Science

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SUMMARYWe have used electron microscopy to further charac terize details of the dynamics of TGN38/41, a protein found to cycle between the ¿ram-Golgi network and the plasma membrane. Immunogold-labeling of NRK cells under steady-state conditions shows the majority of TGN38/41 is localized to the irans-most Golgi cisternae and the iraras-Golgi network. Small amounts of this mol ecule can be detected in early endosomes. Capture of cycling TGN38/41 molecules at the cell surface altered the steady state distribution. This was accomplished by binding TGN38/41 luminal domain antibodies to solid supports (beads), which were introduced to the culture media of cells. As increasing numbers of antigen-antibody complexes formed, the beads were internalized by the 'zippering mechanism' of phagocytosis. This pro vides a system that can address many questions related to the function of TGN38/41 and the trans-Golgi net work itself.

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Section: Steady-state D Istrib Utio N Of Tgn38/41 In Nrk Cellsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%