Th1/17 Cells Infiltrate Murine Cytomegalovirus-Infected Renal Allografts via Virus-Induced CCL20 and Promote Th1 Cells through IL-17A

Dhital, Ravi; Anand, Shashi; Zeng, Qiang; Velazquez, Victoria M.; Boddeda, Srinivasa Rao; Fitch, James; Minz, Ranjana W.; Minz, Mukut; Sharma, Ashish; Cianciolo, Rachel E.; Shimamura, Masako

doi:10.1101/2021.08.05.455061

Cited by 2 publications

(2 citation statements)

References 92 publications

(116 reference statements)

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“…A pre-print version of this manuscript is available at https://www.biorx iv.org/conte nt/10.110 1/2021.08.05.455061v1. 111 However, this manuscript differs from the pre-print version by showing that αIL-17A modulates allograft damage by direct inhibition of IL-17A and not through indirect effects on Treg/Th1 cell infiltrates, and that αIL-17A treatment does not increase viral replication. Therefore, this manuscript differs from the pre-print in content and interpretation.…”

Section: Data Ava I L a B I L I T Y S Tat E M E N Tmentioning

confidence: 85%

“…RNA sequencing data are deposited in the NCBI Gene Expression Omnibus 110 (accession #GSE179788). A pre‐print version of this manuscript is available at https://www.biorxiv.org/content/10.1101/2021.08.05.455061v1 111 . However, this manuscript differs from the pre‐print version by showing that αIL‐17A modulates allograft damage by direct inhibition of IL‐17A and not through indirect effects on Treg/Th1 cell infiltrates, and that αIL‐17A treatment does not increase viral replication.…”

Section: Data Availability Statementmentioning

confidence: 97%

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Murine cytomegalovirus promotes renal allograft inflammation via Th1/17 cells and IL-17A

Dhital

Anand

Graber

et al. 2022

American Journal of Transplantation

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Human cytomegalovirus (HCMV) infection is associated with renal allograft failure. Allograft damage in animal models is accelerated by CMV‐induced T helper 17 (Th17) cell infiltrates. However, the mechanisms whereby CMV promotes Th17 cell‐mediated pathological organ inflammation are uncharacterized. Here we demonstrate that murine CMV (MCMV)‐induced intragraft Th17 cells have a Th1/17 phenotype co‐expressing IFN‐γ and/or TNF‐α, but only a minority of these cells are MCMV specific. Instead, MCMV promotes intragraft expression of CCL20 and CXCL10, which are associated with recruitment of CCR6+CXCR3+ Th17 cells. MCMV also enhances Th17 cell infiltrates after ischemia–reperfusion injury, independent of allogeneic responses. Pharmacologic inhibition of the Th17 cell signature cytokine, IL‐17A, ameliorates MCMV‐associated allograft damage without increasing intragraft viral loads or reducing MCMV‐specific Th1 cell infiltrates. Clinically, HCMV DNAemia is associated with higher serum IL‐17A among renal transplant patients with acute rejection, linking HCMV reactivation with Th17 cell cytokine expression. In summary, CMV promotes allograft damage via cytokine‐mediated Th1/17 cell recruitment, which may be pharmacologically targeted to mitigate graft injury while preserving antiviral T cell immunity.

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Section: Data Ava I L a B I L I T Y S Tat E M E N Tmentioning

confidence: 85%

Section: Data Availability Statementmentioning

confidence: 97%

Murine cytomegalovirus promotes renal allograft inflammation via Th1/17 cells and IL-17A

Dhital

Anand

Graber

et al. 2022

American Journal of Transplantation

Self Cite

View full text Add to dashboard Cite

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Relationship Between Human Cytomegalovirus and IL-17A in Iraqi Women with Polycystic Ovary Syndrome

Hadi Khorsheed,

Al-shibly,

Gatea

2024

Al-Rafidain J Med Sci

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Background: Evidence indicates a potential link between PCOS and low-grade infections. IL-17, also known as IL-17A, is an essential immunological regulator, especially in disorders such as polycystic ovarian syndrome (PCOS). The human cytomegalovirus is a β-herpesvirus that causes inflammation and remains dormant in the host for life. The cytomegalovirus has been central to several PCOS-related concepts. The role of IL-17A in CMV infection remains unknown. Objective: To establish the correlation between PCOS and CMV, as well as the connection between PCOS and serum levels of IL17A. Methods: A case-control study included 60 women with PCOS compared to 40 healthy controls. Samples were analyzed regarding CMV via the real-time PCR technique. Furthermore, the ELISA technique measured serum levels of the IL-17A cytokine. Every sample was taken between September 2023 and January 2024. Results: Positive results for CMV were seen in 50 (83.3%) of patients with PCOS compared with 10 (16.7%) who had negative results, while 6 (15.0%) of healthy control subjects had positive results and 34 (85.0%) had negative results; the difference was highly significant. Furthermore, women with polycystic ovary syndrome had a significantly higher IL-17A serum level when compared to healthy controls. Conclusions: In Iraqi women, HCMV infection in patients with PCOS can be considered a risk factor. Moreover, the results show that IL-17A is an excellent prognostic marker of polycystic ovary syndrome.

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Th1/17 Cells Infiltrate Murine Cytomegalovirus-Infected Renal Allografts via Virus-Induced CCL20 and Promote Th1 Cells through IL-17A

Cited by 2 publications

References 92 publications

Murine cytomegalovirus promotes renal allograft inflammation via Th1/17 cells and IL-17A

Murine cytomegalovirus promotes renal allograft inflammation via Th1/17 cells and IL-17A

Relationship Between Human Cytomegalovirus and IL-17A in Iraqi Women with Polycystic Ovary Syndrome

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