Th17 / Treg ratio: A prospective study in a group of pregnant women with preeclampsia and fetal growth restriction

Braga, António; Neves, Esmeralda; Guimarães, Judite; Braga, Jorge; Vasconcelos, Carlos

doi:10.1016/j.jri.2023.104122

Cited by 2 publications

(1 citation statement)

References 57 publications

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“…33 The imbalanced distribution of Treg and Th17 cells, leading to an increase in the Th17/Treg ratio, is believed to cause various pregnancy-related diseases, including RSA, intrahepatic cholestasis of pregnancy, and preeclampsia. [34][35][36] To the best of our knowledge, this study is the first to investigate the Th17/Treg ratio in patients with UI and find that the Th17/Treg ratio in the UI group shifted toward a direction favorable for Th17 cells, disrupting the balance between Treg and Th17 cells. Moreover, as a diagnostic biomarker for UI, the Th17/Treg ratio was found to be superior to the use of Treg or Th17 cells alone.…”

Section: Discussionmentioning

confidence: 68%

Regulatory T Cell and T Helper 17 Cell Imbalance in Patients with Unexplained Infertility

Lu,

Zhang

2024

IJWH

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Purpose Female infertility is a global health concern. The aim of this study was to investigate the relationship between regulatory T (Treg) cells and helper T cells 17 (Th17) in peripheral blood and unexplained infertility (UI). In addition, we explored potential valuable diagnostic biomarkers for patients with UI and ascertained whether Treg and Th17 cells are associated with primary and secondary UI. Patients and Methods The patients underwent standard fertility evaluation test, including blood tests, ultrasound examination, fallopian tube tests, ovulation assessment, and male partner’s semen analysis. According to the inclusion and exclusion criteria, this study enrolled 37 patients with UI (30 with primary UI and 7 with secondary UI) and 26 age-matched healthy volunteers as the control group. Flow cytometry was used to detect the frequency of Treg and Th17 cells. The area under the receiver operating characteristic curve (AUC) with a 95% confidence interval (CI) was used to assess the diagnostic performance. An AUC > 0.800 indicated good diagnostic performance. Results The percentage of Treg decreased significantly, whereas the percentage and absolute count of Th17 cells increased. Moreover, the Th17/Treg ratio in patients with UI increased significantly. As a diagnostic biomarker for UI, the Th17/Treg ratio exhibited remarkable diagnostic performance (AUC: 0.813 (95% CI = 0.709–0.917)). However, the percentages and absolute counts of Treg and Th17 cells in the peripheral blood of women with primary and secondary UI, as well as their Th17/Treg ratios, did not differ significantly. Conclusion The distribution of Treg and Th17 cells is imbalanced in patients with UI. Therefore, the Th17/Treg ratio may be a promising indicator of UI. However, there were no significant differences in the distribution of Treg and Th17 cells between women with primary and secondary UI.

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Section: Discussionmentioning

confidence: 68%

Regulatory T Cell and T Helper 17 Cell Imbalance in Patients with Unexplained Infertility

Lu,

Zhang

2024

IJWH

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Maternal Soluble Programmed Death Ligand-1 (sPD-L1) and T-regulatory Cells (Tregs) Alteration in Preeclampsia: A Cross-Sectional Study From Eastern India

Dash,

Nayak,

Koppisetty

2024

Cureus

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Background Studies have shown that aberrant reactions of the immune system play an important role in the pathogenesis of preeclampsia. The immune checkpoint molecules programmed cell death protein 1/programmed death-ligand 1 (PD-1/PD-L1) system and the T-regulatory cells (Tregs) system are decisive in the regulation of immune responses and can be the target molecules in preeclampsia. In this study, an attempt has been made to evaluate the soluble PD-L1 (sPD-L1) in the serum of preeclampsia cases and correlate it with Tregs and inflammatory markers to have an insight into the link between these immunomodulatory molecules in the pathogenesis of preeclampsia. Materials and methods Ten normal fertile women, 20 trimester-matched normal pregnancy cases, and 20 preeclampsia cases were enrolled in the study. Serum sPD-L1, transforming growth factor beta 1 (TGF-β1), and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). High-sensitive C-reactive protein (hsCRP) was estimated using a clinical biochemistry autoanalyzer. Tregs were evaluated using flow cytometry. Results and discussion The immune checkpoint molecule PD-L1 inversely correlated with Tregs in preeclampsia cases. Associated inflammation was seen by raised IL-6 and hsCRP. The breakdown of immunological tolerance is mainly caused by the dysregulating the Tregs/Th17 balance, which leads to conditions of autoimmunity and chronic inflammatory disorders. PD-L1 can be the link between this immunological misbalance. Conclusion Our study, showing an increase in sPD-L1 and TGF and a decrease in Tregs with an increase in inflammatory markers like IL-6 and hsCRP levels in preeclampsia, has potential implications for early diagnosis and management of the condition. PD-L1 and Tregs can be target molecules for early management of preeclampsia.

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Th17 / Treg ratio: A prospective study in a group of pregnant women with preeclampsia and fetal growth restriction

Cited by 2 publications

References 57 publications

Regulatory T Cell and T Helper 17 Cell Imbalance in Patients with Unexplained Infertility

Regulatory T Cell and T Helper 17 Cell Imbalance in Patients with Unexplained Infertility

Maternal Soluble Programmed Death Ligand-1 (sPD-L1) and T-regulatory Cells (Tregs) Alteration in Preeclampsia: A Cross-Sectional Study From Eastern India

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