Th2 Responses in OVA-Sensitized BALB/c Mice Are Down-Modulated By Mycobacterium bovis BCG Treatment

Gouveia, Ana Cláudia Carvalho; Brugiolo, Alessa Sin Singer; Alves, Caio César de Souza; Silva, Flávia Márcia Castro; Mesquita, Felipe Pereira; Gameiro, Jacy; Ferreira, Ana Paula

doi:10.1007/s10875-012-9746-4

Cited by 14 publications

(19 citation statements)

References 47 publications

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“…Serum was separated from blood samples obtained 24 hr and 48 hr after the last challenge. Anti‐OVA‐specific IgE and IgG2a were determined on serum, by optical density measurement at 492 nm in a microplate reader (SpectraMax 190; Molecular Devices, Sunnyvale, CA), as described elsewhere …”

Section: Methodsmentioning

confidence: 99%

“…Eosinophil peroxidase activity in lung homogenates was determined as described elsewhere. 21 To evaluate MPO, activity equal parts of homogenate supernatant and Ophenylenediamine solution in 10 mM citrate (pH 5Á5), followed by the addition of H 2 O 2 (20%). The reaction was stopped with H 2 SO 4 , and the absorbance was measured at 492 nm on a microplate reader (SpectraMax 190).…”

Section: Eosinophil Peroxidase and Myeloperoxidase Activitymentioning

confidence: 99%

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Obesity affects peripheral lymphoid organs immune response in murine asthma model

et al. 2019

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Summary Asthma and obesity present rising incidence, and their concomitance is a reason for concern, as obese individuals are usually resistant to conventional asthma treatments and have more exacerbation episodes. Obesity affects several features in the lungs during asthma onset, shifting the T helper type 2 (Th2)/eosinophilic response towards a Th17/neutrophilic profile. Moreover, those individuals can present reduced atopy and delayed cytokine production. However, the impact of obesity on follicular helper T (Tfh) cells and B cells that could potentially result in antibody production disturbances are still unclear. Therefore, we aimed to assess the peripheral response to ovalbumin (OVA) in a concomitant model of obesity and asthma. Pulmonary allergy was induced, in both lean and obese female BALB/c mice, through OVA sensitizations and challenges. Mediastinal lymph nodes (MLNs) and spleen were processed for immunophenotyping. Lung was used for standard allergy analysis. Obese‐allergic mice produced less anti‐OVA IgE and more IgG2a than lean‐allergic mice. Dendritic cells (CD11c+ MHCIIhigh) expressed less CD86 and more PDL1 in obese‐allergic mice compared with lean‐allergic mice, in the MLNs. Meanwhile, B cells (CD19+ CD40+) were more frequent and the amount of PDL1/PD1+ cells was diminished by obesity, with the opposite effects in the spleen. Tfh cells (CD3+ CD4+ CXCR5+ PD1+) expressing FoxP3 were more frequent in obese mice, associated with the predominance of Th (CD3+ CD4+) cells expressing interleukin‐4/GATA3 in the MLNs and interleukin‐17A/RORγT in the spleen. Those modifications to the main components of the germinal centers could be resulting in the increased IgG2a production, which – associated with the Th17/neutrophilic profile – contributes to asthma worsening and represents an important target for future treatment strategies.

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Section: Methodsmentioning

confidence: 99%

Section: Eosinophil Peroxidase and Myeloperoxidase Activitymentioning

confidence: 99%

Obesity affects peripheral lymphoid organs immune response in murine asthma model

et al. 2019

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“…Mice that received 3 labiar Ox challenges following sensitization showed an increase in eosinophil peroxidase activity in the labiar tissue at 24 hours and 120 hours (A); to measure eosinophil peroxidase activity (EPO), we adapted previously described methods [56,57]; excised labia were preserved at -80°C in 0.5% hexadecyltrimethyl ammonium bromide in phosphate-buffered saline, homogenized, freeze-thawed three times, centrifuged twice, first at 4750 rpm and next at 14000 rpm for 4 minutes at 4°C, and 50µl of the supernatant mixed with 100µl of 16mM o-phenylenediamene, 50mM-Tris-HCL, 0.01% H 2 O 2 . The reaction was stopped with 1M H 2 SO 4 after 15 minutes and OD measurements taken at 490 nm.…”

Section: Supporting Informationmentioning

confidence: 99%

Contact Hypersensitivity to Oxazolone Provokes Vulvar Mechanical Hyperalgesia in Mice

et al. 2013

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The interplay among pain, allergy and dysregulated inflammation promises to yield significant conceptual advances in immunology and chronic pain. Hapten-mediated contact hypersensitivity reactions are used to model skin allergies in rodents but have not been utilized to study associated changes in pain perception in the affected skin. Here we characterized changes in mechanical hyperalgesia in oxazolone-sensitized female mice challenged with single and repeated labiar skin exposure to oxazolone. Female mice were sensitized with topical oxazolone on their flanks and challenged 1-3 times on the labia. We then measured mechanical sensitivity of the vulvar region with an electronic pressure meter and evaluated expression of inflammatory genes, leukocyte influx and levels of innervation in the labiar tissue. Oxazolone-sensitized mice developed vulvar mechanical hyperalgesia after a single labiar oxazolone challenge. Hyperalgesia lasted up to 24 hours along with local influx of neutrophils, upregulation of inflammatory cytokine gene expression, and increased density of cutaneous labiar nerve fibers. Three daily oxazolone challenges produced vulvar mechanical hyperalgesic responses and increases in nerve density that were detectable up to 5 days post-challenge even after overt inflammation resolved. This persistent vulvar hyperalgesia is resonant with vulvodynia, an understudied chronic pain condition that is remarkably prevalent in 18-60 year-old women. An elevated risk for vulvodynia has been associated with a history of environmental allergies. Our pre-clinical model can be readily adapted to regimens of chronic exposures and long-term assessment of vulvar pain with and without concurrent inflammation to improve our understanding of mechanisms underlying subsets of vulvodynia and to develop new therapeutics for this condition.

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“…It has been suggested that the inability of BCG revaccination to modulate atopic responses may be related to an individual's age at the time of antigen exposure and/or the genetic background of the study subjects 2 . In animal models, the effect of the modulation of the Th2 response was protective and improved parameters related to allergic conditions in animals exposed to microbial antigens before allergen sensitization 5 . Children with respiratory allergies who received neonatal BCG in Salvador were protected against asthma 8 .…”

Section: A B C D E Fmentioning

confidence: 99%

“…Nevertheless, there is increasing evidence that BCG vaccination modulates several aspects of atopy in experimental models of asthma and humans 4 . Mice immunized with BCG and sensitized intranasally with ovalbumin (OVA) had a lower percentage of eosinophils in the bronchoalveolar lavage, reduced levels of total and allergenspecific serum immunoglobulin E (IgE), decreased levels of T-helper 2 (Th2) cytokine, decreased lung inflammation, as well as increased levels of interferon gamma (IFN-γ), tumor necrosis factor beta (TGF-β), and interleukin-10 (IL-10) 5 . Increased levels of allergen-specific serum IgE and eosinophilia are hallmarks of atopic asthma, and the former is correlated with the severity of clinical symptoms 6 .…”

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confidence: 99%

Impact of Bacille Calmette-Guérin revaccination on serum IgE levels in a randomized controlled trial

Peleteiro

Oliveira

Conceição

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Th2 Responses in OVA-Sensitized BALB/c Mice Are Down-Modulated By Mycobacterium bovis BCG Treatment

Abstract: BCG treatment inhibits features of allergic airway inflammation and the results suggest that the mechanism underlying the down-regulatory effects of BCG on OVA-induced airway inflammation appear to be associated with the induction of both Th1 and T regulatory immune responses.

Cited by 14 publications

References 47 publications

Obesity affects peripheral lymphoid organs immune response in murine asthma model

Obesity affects peripheral lymphoid organs immune response in murine asthma model

Contact Hypersensitivity to Oxazolone Provokes Vulvar Mechanical Hyperalgesia in Mice

Impact of Bacille Calmette-Guérin revaccination on serum IgE levels in a randomized controlled trial

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