Thailandenes, Cryptic Polyene Natural Products Isolated from <i>Burkholderia thailandensis</i> Using Phenotype-Guided Transposon Mutagenesis

Park, Jong-Duk; Moon, Kyuho; Miller, Cheryl N.; Rose, Jessica; Xu, Fei; Ebmeier, Christopher C.; Jacobsen, Jeremy; Mao, Dainan; Old, William M.; DeShazer, David; Seyedsayamdost, Mohammad R.

doi:10.1021/acschembio.9b00883

Cited by 13 publications

(20 citation statements)

References 52 publications

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“…Our results support that the N-oxide HMAQs join the arsenal of antimicrobial compounds produced by B. thailandensis that promote its ability to inhibit growth of other species, such as bactobolin (13), malleilactone (15,16), and thailandenes (19). This produces a variety of HMAQs with side chains of varying length and saturation (22,39).…”

Section: Discussionsupporting

confidence: 76%

“…pseudomallei (18). B. thailandensis also produces thailandenes, a group of polyenes with activity against Gram-positive bacteria (19). As with many bacterial natural products, malleilactone and thailandenes are not produced in standard laboratory conditions (15,16,19).…”

Section: Introductionmentioning

confidence: 99%

“…B. thailandensis also produces thailandenes, a group of polyenes with activity against Gram-positive bacteria (19). As with many bacterial natural products, malleilactone and thailandenes are not produced in standard laboratory conditions (15,16,19). Studies of these molecules were possible through genetic (15,16) or chemical (20) elicitation of the gene clusters or through phenotype-based screening approaches (19).…”

Section: Introductionmentioning

confidence: 99%

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Burkholderia thailandensismethylated hydroxy-alkylquinolines: biosynthesis and antimicrobial activity in co-cultures

Klaus

Majerczyk

Eppler

et al. 2020

Preprint

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22Lawrence, KS 66045 23 jrchandler@ku.edu 24 25 2 ABSTRACT (179/250 words) 26 The bacterium Burkholderia thailandensis produces an arsenal of secondary metabolites 27 that have diverse structures and roles in the ecology of this soil-dwelling bacterium. In 28 liquid co-culture experiments, B. thailandensis secretes an antimicrobial that nearly 29 eliminates another soil bacterium, Bacillus subtilis. To identify the antimicrobial, we used 30 a transposon mutagenesis approach. This screen identified antimicrobial-defective 31 mutants with insertions in the hmqA, hmqC and hmqF genes involved in biosynthesis of a 32 family of 2-alkyl-4(1H)-quinolones called 4-hydroxy-3-methyl-2-alkenylquinolines 33 (HMAQs), which are closely related to the Pseudomonas aeruginosa 4-hydroxy-2-34 alkylquinolines (HAQs). Insertions also occurred in the previously uncharacterized gene 35 BTH_II1576. Results confirm that BTH_II1576 is involved in generating N-oxide 36 derivatives of HMAQs (HMAQ-NO) in B. thailandensis and that HMAQ-NOs are 37 sufficient to eliminate B. subtilis in co-cultures. Moreover, synthetic HMAQ-NO is ~50-38 fold more active than HMAQ. Both the methyl group and the length of the carbon side 39 chain account for high activity of HMAQ-NO against B. subtilis. The results provide new 40 information on the biosynthesis and activities of HMAQs and reveal new insight into 41 how these molecules might be important for the ecology of B. thailandensis. 42 43 44 45 46 47 48 IMPORTANCE (120 words) 50 The soil bacterium Burkholderia thailandensis produces 2-alkyl-4(1H)-quinolones, 51 mostly methylated 4-hydroxy-alkenylquinolines, a family of relatively unstudied 52 metabolites similar to molecules also synthesized by Pseudomonas aeruginosa. Several 53 of the methylated 4-hydroxy-alkenylquinolines have antimicrobial activity against other 54 species. We show that N-oxidated methyl-alkenylquinolines are particularly antimicrobial 55 and sufficient to kill Bacillus subtilis in co-cultures. We confirmed their biosynthesis 56 requires the previously unstudied protein HmqL. These results provide new information 57 about the biology of 2-alkyl-4(1H)-quinolones, particularly the methylated 4-hydroxy-58 alkenylquinolines, which are unique to B. thailandensis. This study also has importance 59 for understanding B. thailandensis secondary metabolites and has implications for 60 potential therapeutic development. 61 62 64 pathogens, B. pseudomallei and B. mallei, which are the causative agents of melioidosis 65 and glanders, respectively (1, 2). B. pseudomallei is also a saprophyte and causes 66 respiratory or skin infections in humans following exposure to organisms in the 67 environment, such as through skin contact with soil (3). B. mallei is a host-adapted 68 pathogen and is spread to humans from horses and other ungulates, in which it is endemic 69 in some regions (4). Because B. pseudomallei and B. mallei are Tier 1 Select Agents and 70 require handling in BSL-3-level laboratory conditions, B. thailandensis is often used as a ...

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Burkholderia thailandensismethylated hydroxy-alkylquinolines: biosynthesis and antimicrobial activity in co-cultures

Klaus

Majerczyk

Eppler

et al. 2020

Preprint

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show abstract

“…Other PKS antibiotics are malleilactone (14,15) and malleicyprol, a more toxic product of the malleilactone biosynthetic gene cluster (16), which contribute to virulence of B. pseudomallei (17). B. thailandensis also produces thailandenes, a group of polyenes with activity against Gram-positive bacteria (18). As with many bacterial natural products, malleilactone and thailandenes are not produced under standard laboratory conditions (14,15,18).…”

mentioning

confidence: 99%

“…B. thailandensis also produces thailandenes, a group of polyenes with activity against Gram-positive bacteria (18). As with many bacterial natural products, malleilactone and thailandenes are not produced under standard laboratory conditions (14,15,18). Studies of these molecules were possible through genetic (14,15) or chemical (19) elicitation of the gene clusters or through phenotype-based screening approaches (18).…”

mentioning

confidence: 99%

Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures

Klaus

Majerczyk

Moon

et al. 2020

Appl Environ Microbiol

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The bacterium Burkholderia thailandensis produces an arsenal of secondary metabolites that have diverse structures and roles in the ecology of this soil-dwelling bacterium. In co-culture experiments, B. thailandensis strain E264 secretes an antimicrobial that nearly eliminates another soil bacterium, Bacillus subtilis strain 168. To identify the antimicrobial, we used a transposon mutagenesis approach. This screen identified antimicrobial-defective mutants with insertions in the hmqA, hmqC, and hmqF genes involved in biosynthesis of a family of 2-alkyl-4(1H)-quinolones called 4-hydroxy-3-methyl-2-alkenylquinolines (HMAQs), which are closely related to the Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs). Insertions also occurred in the previously uncharacterized gene BTH_II1576 (“hmqL”). Results confirm that BTH_II1576 is involved in generating N-oxide derivatives of HMAQs (HMAQ-NO). Synthetic HMAQ-NO is active against B. subtilis 168, showing ∼50-fold more activity than HMAQ. Both the methyl group and the length of the carbon side chain account for high activity of HMAQ-NO. The results provide new information on the biosynthesis and activities of HMAQs and reveal new insight into how these molecules might be important for the ecology of B. thailandensis. IMPORTANCE The soil bacterium Burkholderia thailandensis produces 2-alkyl-4(1H)-quinolones, mostly methylated 4-hydroxy-alkenylquinolines, a family of relatively unstudied metabolites similar to molecules also synthesized by Pseudomonas aeruginosa. Several of the methylated 4-hydroxy-alkenylquinolines have antimicrobial activity against other species. We show that Bacillus subtilis strain 168 is particularly susceptible to N-oxidated methyl-alkenylquinolines (HMAQ-NO). We confirmed HMAQ-NO biosynthesis requires the previously unstudied protein HmqL. These results provide new information about the biology of 2-alkyl-4(1H)-quinolones, particularly the methylated 4-hydroxy-alkenylquinolines, which are unique to B. thailandensis. This study also has importance for understanding B. thailandensis secondary metabolites and has implications for potential therapeutic development.

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Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery

Covington

Seyedsayamdost

2022

Methods in Enzymology

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Thailandenes, Cryptic Polyene Natural Products Isolated from Burkholderia thailandensis Using Phenotype-Guided Transposon Mutagenesis

Cited by 13 publications

References 52 publications

Burkholderia thailandensismethylated hydroxy-alkylquinolines: biosynthesis and antimicrobial activity in co-cultures

Burkholderia thailandensismethylated hydroxy-alkylquinolines: biosynthesis and antimicrobial activity in co-cultures

Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures

Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery

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