Thainema gen. nov. (Leptolyngbyaceae, Synechococcales): A new genus of simple trichal cyanobacteria isolated from a solar saltern environment in Thailand

Rasouli-Dogaheh, Somayeh; Komárek, Jiří; Chatchawan, Thomrat; Hauer, Tomáš

doi:10.1371/journal.pone.0261682

Cited by 9 publications

(8 citation statements)

References 91 publications

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“…the bPTP and ABGD methods. This result is consistent with previous studies, where researchers discovered more operational taxonomic units using GMYC analysis compared to other species delimitation methods (Esselstyn et al 2012;Fujisawa & Barraclough 2013;Kekkonen & Hebert 2014;Miralles & Vences 2013;Talavera et al 2013;Tang et al 2014;Lorén et al 2018;Rasouli-Dogaheh et al 2022). In addition, among the different approaches in this study, the ITS secondary structure was the most sensitive method to separate genetic groups in Cyanobacteria.…”

Section: Khargia Italicasupporting

confidence: 92%

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Khargia gen. nov., a new genus of simple trichal Cyanobacteria from the Persian Gulf

Rasouli-Dogaheh¹,

Noroozi²,

Khansha³

et al. 2023

Fottea

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Information regarding the diversity of Cyanobacteria in many parts of the world is still minimal. One example of a region that has not yet been widely studied is south-western Asia, including the region of the Persian Gulf. A culture-dependent study of cyanobacterial diversity in a rainwater basin on Kharg Island enabled the isolation of a novel taxon, previously unnamed, from a simple trichal cyanobacterium. Further comparisons showed the existence of closely related strains/taxa from other parts of the world, namely the strains CCALA 945 isolated from South Italy and "Leptolyngbya india" from India. Herein, we have thus described the new genus Khargia and the new species Khargia iranica. Other strains, isolated by other authors, were included in the Khargia genus as additional species: Khargia italica and Khargia indica. The recognition of the new genus was based on morphological evaluations (identification by both light and electron microscopy), the phylogenetic analyses based on the 16S rRNA gene, and species delimitation based on Automatic Barcode Gap Definition (ABGD), Generalized Mixed Yule Coalescent (GMYC), bayesian version of Poisson Tree Processes (bPTP), and the secondary structure of 16S-23S rRNA ITS region of the studied strains.

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Section: Khargia Italicasupporting

confidence: 92%

“…This algorithm tends to do better than GMYC when interspecific distances are small (Zhang et al 2013). Indeed, it is documented that species delimitation methods can effectively bridge the gap between the traditional taxonomy and molecular approaches (Dvořák et al 2018;Rasouli-Dogaheh et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Khargia gen. nov., a new genus of simple trichal Cyanobacteria from the Persian Gulf

Rasouli-Dogaheh¹,

Noroozi²,

Khansha³

et al. 2023

Fottea

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“…In this circumstance, the p-distance method based on the 16S rRNA gene cannot be used as the only absolute basis for separating terminal taxonomic levels (i.e., genera and species). Therefore, apart from utilizing the gold standard 16S-rRNA-gene-based phylogeny, other informative molecular characteristics-including the analysis of the p-distance dissimilarity of 16S-23S ITS and the prediction of secondary structures-have proven their usefulness as strong additional sources of evidence to support the high similarity values given by the 16S rRNA gene sequence comparison across cyanobacterial lineages [55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Stenomitos nagquensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) from a Meadow Wetland in the Tibet Plateau: A Novel Species Studied Based on a Polyphasic Approach

et al. 2023

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A novel cyanobacterial strain (CSML-F035) here described as Stenomitos nagquensis sp. nov. was isolated from a plateau meadow wetland habitat in the Tibet Autonomous Region. The novel strain was studied based on a polyphasic approach by combining characteristics of cell morphology and evidence from molecular data and ecology. Phylogenetic analyses based on the 16S rRNA and rbcL genes indicated that the novel strain is a member of the genus Stenomitos (Leptolyngbyaceae) but is positioned in a unique clade, with strongly supported values from BI, ML, and NJ methods. The percentage similarity of the 16S rRNA gene sequence of CSML-F035 compared with known strains of Stenomitos was >97%. Morphologically, S. nagquensis sp. nov. possesses overlapping morphological characteristics with other Stenomitos species, with thin trichomes enclosed in a firm, colorless, hyaline, persistent mucilage. However, the strain appeared to have slightly smaller cells compared to most known species of Stenomitos. Further analyses of the 16S–23S ITS region showed dissimilarity at 11.2–22.8% compared to known species of Stenomitos. The secondary helices—including D1–D1, V2, Box–B, and V3—that were predicted based on the 16S–23S ITS region for Stenomitos nagquensis sp. nov. were also distinct, thereby providing strong additional support for the separation of this species. This study highlights the importance of unexplored habitats in the Tibet region in which cyanobacterial diversity can be deemed high, with many potential new species that remain poorly explored.

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“…Komárek et al (2014) have discussed the possibility of the existence of cryptic genera (cryptogenera) in cyanobacteria, particularly in simple thin filamentous taxa. These are clearly distinct evolutionary taxa, which can be identified through molecular phylogenies Mai et al, 2018;Mikhailyuk et al, 2022;Osorio-Santos et al, 2014;Pietrasiak et al, 2019Pietrasiak et al, , 2021Rasouli-Dogaheh et al, 2022;Sciuto et al, 2017;Shalygin et al, 2020;Song et al, 2015;Vinogradova & Mikhailyuk, 2018).…”

Section: Analyses Of the Its Rrna Region And Secondary Structuresmentioning

confidence: 99%

Gansulinema gen. nov. and Komarkovaeasiopsis gen. nov.: Novel Oculatellacean genera (Cyanobacteria) isolated from desert soils and hot spring

Cai,

Li,

Chen

et al. 2024

Journal of Phycology

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To increase the understanding of simple thin filamentous cyanobacteria in harsh environmental areas, we previously isolated and identified four strains (XN101, XN102, GS121, NX122) from desert soils and hot spring in China. As a result, two new Oculatellacean genera of these four strains, Gansulinema gen. nov. and Komarkovaeasiopsis gen. nov., are described based on a polyphasic approach. The ultrastructure of these strains showed a similar arrangement of peripheral thylakoids with three to four parallel layers, indicating that they belonged to the orders Nodosilineales, Oculatellales, or Leptolyngbyales. In the 16S rRNA gene phylogeny, two sequences of the Gansulinema strains and the two sequences of the Komarkovaeasiopsis strains formed two independent and robust clusters, within the order Oculatellales. The 16S rRNA gene sequences of strains of Komarkovaeasiopsis and Gansulinema showed low identity to each other (≤93.2%) and to other sequences of the Oculatellacean genera (≤94.5% and ≤93.3%, respectively). Furthermore, the 16S–23S internal transcribed spacer rRNA region secondary structures of strains of Komarkovaeasiopsis and Gansulinema were not consistent with all existing descriptions of Oculatellacean taxa. These data suggest that cyanobacterial communities are rich sources of new taxa in under‐exploited areas, such as desert soils and hot spring in China.

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Thainema gen. nov. (Leptolyngbyaceae, Synechococcales): A new genus of simple trichal cyanobacteria isolated from a solar saltern environment in Thailand

Cited by 9 publications

References 91 publications

Khargia gen. nov., a new genus of simple trichal Cyanobacteria from the Persian Gulf

Khargia gen. nov., a new genus of simple trichal Cyanobacteria from the Persian Gulf

Stenomitos nagquensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) from a Meadow Wetland in the Tibet Plateau: A Novel Species Studied Based on a Polyphasic Approach

Gansulinema gen. nov. and Komarkovaeasiopsis gen. nov.: Novel Oculatellacean genera (Cyanobacteria) isolated from desert soils and hot spring

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