Thawing of cryopreserved hematopoietic progenitor cells from apheresis with a new dry‐warming device

Triana, Elvira; Ortega, Sandra; Azqueta, Carmen; Pomares, Helena; Valdivia, Elena; Duarte, Rafael F.; Massuet, L; Martín-Henao, G A

doi:10.1111/j.1537-2995.2012.03669.x

Cited by 11 publications

(11 citation statements)

References 12 publications

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“…The most commonly used thawing procedure is direct immersion of the frozen sample container in a warmed water bath (37 C), which produces rapid thawing. Dry thawing systems have also been developed in response to constraints imposed by current cleanroom procedures and clinical practice [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

Kilbride

Meneghel

2020

Methods in Molecular Biology

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From early dry-ice-based freezers and passive coolers, cryopreservation devices have come a long way. With increasing interest in the field of cryobiology from new scientific applications, the importance of reliable, traceable, and reproducible cold chain devices is sure to increase, ensuring more precise cryopreservation and enabling better post-thaw outcomes, both for the user and for biological samples. As with any cryopreservation process, it is important to optimize each part of the cold chain for each lab’s biological samples, cryocontainers used, and logistical restraints. In this chapter we describe how freezing technology can be used for cryopreservation of cells.

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Section: Introductionmentioning

confidence: 99%

Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

Kilbride

Meneghel

2020

Methods in Molecular Biology

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“…Other method, that has been studied up to now, is the application of dry heat. These studies identified that samples thawed using dry heat had similar viabilities and clonogenic potential to those thawed using the standard water procedure (56,57). The different thawing temperature (0, 20 and 37 °C for 20 min) did not uncover any statistically significant changes on post-thawed cells (58).…”

Section: Cell Thawingmentioning

confidence: 98%

Cryopreservation of Dental Stem Cells

Pilbauerova

Suchánek

2018

Acta Med. (Hradec Kralove, Czech Repub.)

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Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies. The objective of this review is to compare them and highlight their advantages and disadvantages.

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“…The use of this type of equipment should eliminate the subjective assessment of thawing and provide a consistent procedure that requires no specific expertise on the part of the operator. In addition to cryobags ( 48 , 184 , 185 ), non-cellular therapeutic materials such as plasma samples can be thawed using this type of equipment ( 186 ).…”

Section: Thawing and Recoverymentioning

confidence: 99%

Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

2020

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Cryopreservation is a key enabling technology in regenerative medicine that provides stable and secure extended cell storage for primary tissue isolates and constructs and prepared cell preparations. The essential detail of the process as it can be applied to cell-based therapies is set out in this review, covering tissue and cell isolation, cryoprotection, cooling and freezing, frozen storage and transport, thawing, and recovery. The aim is to provide clinical scientists with an overview of the benefits and difficulties associated with cryopreservation to assist them with problem resolution in their routine work, or to enable them to consider future involvement in cryopreservative procedures. It is also intended to facilitate networking between clinicians and cryo-researchers to review difficulties and problems to advance protocol optimization and innovative design.

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Thawing of cryopreserved hematopoietic progenitor cells from apheresis with a new dry‐warming device

Cited by 11 publications

References 12 publications

Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

Cryopreservation of Dental Stem Cells

Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

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