The 1-Cys peroxiredoxin, a regulator of seed dormancy, functions as a molecular chaperone under oxidative stress conditions

Kim, Sun Young; Paeng, Seol Ki; Nawkar, Ganesh M.; Maibam, Punyakishore; Lee, Eun Seon; Kim, Kang‐San; Lee, Deok Ho; Park, Dong-Jin; Kang, Sun Bin; Kim, Mi Ri; Lee, Joo Hyeong; Kim, Young Hun; Kim, Woe Yeon; Kang, Chang Ho

doi:10.1016/j.plantsci.2011.04.010

Cited by 42 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result corroborates Kim et al (2011), who reported a significant increase in the expression of 1-Cys PrxR during the late stage of seed development. The GPX and PrxR families catalyze the reduction of peroxides and play an important role in antioxidant defense and stress signaling (Matamoros et al, 2010).…”

Section: Stress Response and Defensesupporting

confidence: 92%

“…The GPX and PrxR families catalyze the reduction of peroxides and play an important role in antioxidant defense and stress signaling (Matamoros et al, 2010). Studies have demonstrated that 1-Cys PrxR has both molecular chaperone and peroxidase activities, suggesting that the peroxidase activity is important for gene expression during seed development and germination under normal conditions, whereas the molecular chaperone activity is crucial for tissue protection under stress conditions (Kim et al, 2011). Glyoxalase I (Glo I), involved in cellular detoxification, was also identified (spot 01) in all conditions studied.…”

Section: Stress Response and Defensementioning

confidence: 99%

See 1 more Smart Citation

Research Article A proteomic approach to guarana seed and pericarp maturation.

Souza¹,

Lopez-Losano

Angelo³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Paullinia cupana Kunth var. sorbilis (Mart.) Ducke, the cultivated guarana plant, is native to the Amazon and has been valued for its medicinal, stimulant and energetic properties for centuries. The seeds are the main commercial product of the plant and the source of high amounts of purine alkaloids (caffeine and theobromine) and polyphenols (flavonoids, catechins, and tannins). Proteins involved in the development and maturation of guarana fruits in its native habitat are interesting issues for proteomics. This study presents the proteomic profile of the seed and pericarp of healthy guarana in different maturation stages. Protein contents were higher in the mature seed compared to other stages due to the accumulation of storage proteins -11S globulins. Proteins selected for identification by mass spectrometry are mostly related to stress responses and defense and this is not unexpected for fast growing and differentiating reproductive tissues.

show abstract

Section: Stress Response and Defensesupporting

confidence: 92%

Section: Stress Response and Defensementioning

confidence: 99%

Research Article A proteomic approach to guarana seed and pericarp maturation.

Souza¹,

Lopez-Losano

Angelo³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…1-Cys peroxiredoxins are susceptible to overoxidation of the active-site Cys, which can be observed in 2D gel electrophoresis, since this causes a pI shift of the protein (Pulido et al, 2009). Chaperone activity was increased in the overoxidized form, whereas the reduced form had greater peroxidase activity (Kim et al, 2011). In our experiments, these reduced and oxidized forms likely correspond to the high-and low-pI spots, respectively.…”

Section: Many Of the Extracellular And Intracellular Proteins (Supplementioning

confidence: 51%

“…Besides its antioxidant role during barley germination (Stacy et al, 1999), a chaperone activity was demonstrated for 1-Cys peroxiredoxin from Chinese cabbage (Brassica campestris ssp. pekinensis) seeds (Kim et al, 2011). 1-Cys peroxiredoxins are susceptible to overoxidation of the active-site Cys, which can be observed in 2D gel electrophoresis, since this causes a pI shift of the protein (Pulido et al, 2009).…”

Section: Many Of the Extracellular And Intracellular Proteins (Supplementioning

confidence: 99%

Gibberellic Acid-Induced Aleurone Layers Responding to Heat Shock or Tunicamycin Provide Insight into theN-Glycoproteome, Protein Secretion, and Endoplasmic Reticulum Stress

et al. 2013

View full text Add to dashboard Cite

The growing relevance of plants for the production of recombinant proteins makes understanding the secretory machinery, including the identification of glycosylation sites in secreted proteins, an important goal of plant proteomics. Barley (Hordeum vulgare) aleurone layers maintained in vitro respond to gibberellic acid by secreting an array of proteins and provide a unique system for the analysis of plant protein secretion. Perturbation of protein secretion in gibberellic acid-induced aleurone layers by two independent mechanisms, heat shock and tunicamycin treatment, demonstrated overlapping effects on both the intracellular and secreted proteomes. Proteins in a total of 22 and 178 two-dimensional gel spots changing in intensity in extracellular and intracellular fractions, respectively, were identified by mass spectrometry. Among these are proteins with key roles in protein processing and secretion, such as calreticulin, protein disulfide isomerase, proteasome subunits, and isopentenyl diphosphate isomerase. Sixteen heat shock proteins in 29 spots showed diverse responses to the treatments, with only a minority increasing in response to heat shock. The majority, all of which were small heat shock proteins, decreased in heat-shocked aleurone layers. Additionally, glycopeptide enrichment and N-glycosylation analysis identified 73 glycosylation sites in 65 aleurone layer proteins, with 53 of the glycoproteins found in extracellular fractions and 36 found in intracellular fractions. This represents major progress in characterization of the barley N-glycoproteome, since only four of these sites were previously described. Overall, these findings considerably advance knowledge of the plant protein secretion system in general and emphasize the versatility of the aleurone layer as a model system for studying plant protein secretion.

show abstract

“…More important, baPWV provided an additional prognostic value over conventional risk factors respective modalities alone 39) . In addition, Di Carli and Hachamovitch demonstrated that an integrated approach with cardiac positron emission tomography (PET) and CCTA overcame the discrepancy between anatomical and physiological measurements of stenosis severity 40) .…”

Section: Discussionmentioning

confidence: 99%

Incremental Prognostic Value of Brachial-Ankle Pulse Wave Velocity to Single-Photon Emission Computed Tomography in Patients with Suspected Coronary Artery Disease

Lee

Kim

et al. 2015

J Atheroscler Thromb

View full text Add to dashboard Cite

Aim: Arterial stiffness assessed by brachial-ankle pulse wave velocity (baPWV) is predictive of cardiovascular events. This study was designed to investigate whether baPWV has an additional prognostic value to single-photon emission computed tomography (SPECT) in patients with suspected coronary artery disease (CAD). Methods: A total of 350 subjects (age, 66.2 10.5 years, 53.4% male) with suspected CAD undergoing myocardial SPECT and baPWV within 30 days were retrospectively analyzed. Cardiovascular events, including cardiovascular death, acute coronary syndrome and ischemic stroke, were assessed. Both fixed and reversible perfusion defects on SPECT were considered abnormal myocardial perfusion imaging (MPI) findings. Results: During the median follow-up period of 441 days (interquartile range 169 -719 days), cardiovascular events occurred in 21 patients (6.0%). In multivariable Cox regression analysis, abnormal MPI [hazard ratio (HR), 2.67; 95% confidence interval (CI), 1.21 -10.37; p 0.024] and high baPWV ( ≥1,790 cm/s) (HR, 2.03; 95% CI, 1.08 -6.38; p 0.007) were independent predictors of clinical events even after adjusting for possible confounders. Also, high baPWV had an incremental prognostic value to traditional risk factors and abnormal MPI in predicting cardiovascular events (overall Chi-square, from 24.08 to 27.42; p 0.001). Kaplan -Meier survival curves stratified by baPWV and MPI proved significantly improved prediction of cardiovascular events (log-rank p 0.001). Conclusions: baPWV has an incremental prognostic value to traditional risk factors and MPI. Therefore, baPWV can be used to identify subjects at higher risk of cardiovascular events in patients undergoing SPECT.

show abstract

The 1-Cys peroxiredoxin, a regulator of seed dormancy, functions as a molecular chaperone under oxidative stress conditions

Cited by 42 publications

References 22 publications

Research Article A proteomic approach to guarana seed and pericarp maturation.

Research Article A proteomic approach to guarana seed and pericarp maturation.

Gibberellic Acid-Induced Aleurone Layers Responding to Heat Shock or Tunicamycin Provide Insight into theN-Glycoproteome, Protein Secretion, and Endoplasmic Reticulum Stress

Incremental Prognostic Value of Brachial-Ankle Pulse Wave Velocity to Single-Photon Emission Computed Tomography in Patients with Suspected Coronary Artery Disease

Contact Info

Product

Resources

About