“…Formalin-fixed paraffin sections were used for all histology except for CD98, Ki67, 6, and 1 integrins and laminin 332 immunostaining, which were performed on frozen sections. Antibodies were: rabbit antimouse involucrin (Covance), rabbit anti-mouse cytokeratin 10 (Covance), goat anti-mouse Ki67 (M-19; Santa Cruz Biotechnology, Inc.), rat antiintegrin 1 (MAB1997; Millipore), monoclonal anti-6 (GoH3; R&D Systems), rabbit anti-laminin 332 (SE144, gift of L. Gagnoux-Palacios, Institut National de la Sante et de la Recherche Medicale U634, Nice, France; Vailly et al, 1994), rat anti-mouse CD98 (clone RL388; eBioscience), rat antimouse macrophage Mac 2 (clone CL8942 AP; Cedarlane), rat IgG2a isotype control (eBioscience), and Alexa Fluor 488-or 546-conjugated anti-rat, -mouse, -goat, or -rabbit (Invitrogen).…”