The 10trans, 12cis isomer of conjugated linoleic acid promotes energy metabolism in OLETF rats

Nagao, Koji; Wang, Yu Ming; Inoue, Nao; Han, Seo Young; Buang, Yohanes; Noda, Toshihiro; Kouda, Noriyuki; Okamatsu, Hiroshi; Yanagita, Teruyoshi

doi:10.1016/s0899-9007(03)00060-1

Cited by 81 publications

(51 citation statements)

References 38 publications

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“…These effects can be due to an adipogenesis inhibition which suppresses preadipocyte differentiation (Kennedy et al 2010). The increase of energy expenditure and thermogenesis was largely studied in mice and rodents (West et al 2000;Miner et al 2001;Nagao et al 2003) and was ascribed to an increase of the basal metabolic rate (Kennedy et al 2010). In addition, the antioxidants proprieties of CLA improved immunity and fights cancer (cis-9, trans 11) (Doreau & Chilliard 1997;Zhang et al 2005;Zanini et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary protein concentrations, amino acids and conjugated linoleic acid supplementations on productive performance and lipid metabolism of broiler chicks

Attia

Bovera

Abd-El-Hamid

et al. 2017

Italian Journal of Animal Science

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The study investigated the effect of low-crude protein (CP), essential aminoacids (EAAs) and conjugated linoleic acid (CLA) on broiler growth performance. From 1 to 6 days of age, 196 male broiler chicks fed the same basal diet containing 22.5% of crude protein. From 7-28 days the chicks were assigned to 7 treatments (4 replicates, 7 birds/replicate). The control group fed a 21.5% CP diet, supplemented with DL-methionine (Met) and L-lysine (Lys). The low-CP diet (19% CP) was supplemented with Met þ Lys (2EAAs group) or with Met, Lys, L-arginine, L-tryptophan and L-threonine (5EAAs group); 2EAAs and 5EAAs groups were also supplemented with 0, 2 or 4% CLA. The body weight gain (BWG) was the biggest (p < .01) in the control group; 2EAAs and 2EAAs 4% CLA groups had lower BWG than the three groups fed 5EAAs diets. Diet 5EAAs þ2% CLA induced lower (p < .01) feed conversion ratio (FCR) than 5EAAs þ4% CLA and also showed the lowest (p < .01) protein intake. Reducing CP in broiler diet increased (p < .01) the digestibility of CP. The total protein content of plasma was greater (p < .01) in 2EAAs þ4%CLA and 5EAAs þ4%CLA than in the control. Decreasing the protein content of the diet from 21.5 to 19.0% penalised the growth performance of broilers from 7 to 28 days of age and the administration of CLA at 2 or 4% was not able to support growth. However, addition of 5EAAs without or with 2 or 4% of CLA resulted in recovery of FCR and PCR.ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Effect of dietary protein concentrations, amino acids and conjugated linoleic acid supplementations on productive performance and lipid metabolism of broiler chicks

Attia

Bovera

Abd-El-Hamid

et al. 2017

Italian Journal of Animal Science

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“…[11][12][13][14] These studies called a great deal of attention to the fractionation of CLA isomers.…”

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confidence: 99%

“…9) It was recently reported that c9,t 11-CLA had anticancer activity, 10) and that t 10,c12-CLA participated in the decrease of body fat and the increase of energy expenditure. [11][12][13][14] These studies called a great deal of attention to the fractionation of CLA isomers.…”

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confidence: 99%

Purification of Conjugated Linoleic Acid Isomers through a Process Including Lipase-catalyzed Selective Esterification

Nagao

Yamauchi-Sato

Sugihara

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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A mixture of conjugated linoleic acids (CLAs) was prepared by alkali conjugation of high purity linoleic acid. The preparation contained 45.1 wt% cis-9, trans-11 (c9,t 11)-CLA, 46.8 wt% trans-10, cis-12 (t 10,c12)-CLA, and 5.3 wt% other CLAs. A process comprising Candida rugosa lipase-catalyzed selective esteriˆcation with lauryl alcohol, molecular distillation, and urea adduct fractionation under strict conditions in ethanol was very eŠective for puriˆcation of c9,t 11-and t 10,c12-CLAs. In particular, the urea adduct fractionation e‹ciently eliminated CLAs except c9,t 11-and t 10,c12-isomers. Puriˆcation of c9,t 11-and t 10,c12-CLAs from 1.0 kg of the CLA mixture increased the c9,t 11-CLA purity to 93.1% with 34% recovery of the initial content, and increased the t 10,c12-CLA purity to 95.3% with 31% recovery.Key words: conjugated linoleic acid; Candida rugosa lipase; esteriˆcation; urea adduct fractionation; molecular distillation Conjugated linoleic acid (CLA) is a group of C18 fatty acids (FAs) containing a pair of conjugated double bonds in either the cis or trans conˆguration. A commercialized product contains almost equal amounts of cis-9, trans-11 (c9,t 11)-CLA and trans-10, cis-12 (t 10,c12)-CLA. The mixture of CLA isomers has various physiological activities, such as reduction of the incidence of cancer, 1-3) decrease in body fat content, 4-6) beneˆcial eŠects on atherosclerosis, 7,8) and improvement of immune function.9) It was recently reported that c9,t 11-CLA had anticancer activity, 10) and that t 10,c12-CLA participated in the decrease of body fat and the increase of energy expenditure. [11][12][13][14] These studies called a great deal of attention to the fractionation of CLA isomers.Lipases from Geotrichum candidum and Candida rugosa were recently reported to recognize c9,t 11-CLA more readily than t 10,c12-CLA, and an enzymatic method with these lipases was eŠective for the separation of c9,t 11-and t 10,c12-CLAs. 15,16) In particular, we successfully puriˆed c9,t 11-and t 10,c12-CLAs to 95z based on the total content of the two isomers by a process composed of C. rugosa lipase-catalyzed selective esteriˆcation with lauryl alcohol (LauOH), molecular distillation, and urea adduct fractionation.16) However, the two puriˆed preparations, especially t 10,c12-CLA preparation, contained nearly 10z of CLAs other than c9,t 11-and t 10,c12-isomers.16) In addition, the two puriˆed preparations contained oleic acid. These contaminants are undesirable when studying physicochemical and physiological properties of the two CLAs. We thus attempted to purify the two CLAs without contamination by other isomers. This paper reports that high purity of the two CLAs can be obtained by improving our previous process as follows: a CLA mixture prepared by alkali conjugation of highly puriˆed linoleic acid is used as a starting material, and urea adduct fractionation is done under strict conditions with ethanol.We previously fractionated c9,t 11-and t 10,c12-CLAs from a commercially available CLA mixture. Because the ...

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“…Slight (+3-4%) [14,19] and more elevated increases (+7-16%) [35,36] in energy expenditure have been reported in mice fed a CLA mixture, and in obese rats (+12% with the trans-10, cis-12 CLA) [37], where they resulted in a decrease in fat mass. However it was only in the trained group that feeding a CLA mixture increased energy expenditure in Balb/C mice [21].…”

Section: Effect Of Cla Feedingmentioning

confidence: 95%

Dietary conjugated linoleic acid has limited effects on tissue protein anabolism in sedentary and exercising adult rats

et al. 2006

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-The effects of conjugated linoleic acid isomers (CLA) and endurance training on lean body mass are expected to result from their action on tissue protein metabolism. The aim of this study was to analyze their effects on protein metabolism in 2 muscles, the small intestine and liver of adult rats. Four-month-old male Wistar rats were fed diets containing either no CLA, cis-9, trans-11 CLA isomer (1 g.100 g −1 ), trans-10, cis-12 CLA isomer (1 g.100 g −1 ) or both isomers (1 g.100 g −1 each) for 6 weeks. Half of the rats were subjected to endurance training by running on a treadmill. At the end of this period, the rats were injected with a flooding dose of 13 C-valine to determine protein synthesis rates in the post-absorptive (experiment 1) and in the post-prandial (experiment 2) states. No effect of CLA or endurance training were detected in the small intestine. Training reduced food intake and protein synthesis rates in the liver but no effect was found on the protein synthesis rates in muscles. In the post-absorptive state, protein synthesis rate was increased by feeding the trans-10, cis-12 CLA isomer alone in the liver (+9%) or in combination with the cis-9, trans-11 isomer in the gastrocnemius (+30%), mostly in sedentary rats. In the post-prandial state, the cis-9, trans-11 CLA isomer tended to reduce the protein synthesis rate in the gastrocnemius muscle. However, no effect of CLA was found on muscle protein amounts. In conclusion, CLA isomers would have limited but differential effects on tissue protein metabolism in adult rats. conjugated linoleic acid / protein metabolism / exercise / muscle / liver / intestine

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The 10trans, 12cis isomer of conjugated linoleic acid promotes energy metabolism in OLETF rats

Cited by 81 publications

References 38 publications

Effect of dietary protein concentrations, amino acids and conjugated linoleic acid supplementations on productive performance and lipid metabolism of broiler chicks

Effect of dietary protein concentrations, amino acids and conjugated linoleic acid supplementations on productive performance and lipid metabolism of broiler chicks

Purification of Conjugated Linoleic Acid Isomers through a Process Including Lipase-catalyzed Selective Esterification

Dietary conjugated linoleic acid has limited effects on tissue protein anabolism in sedentary and exercising adult rats

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