The 125th Anniversary of the Griess Reagent

Иванов, В. М.

doi:10.1023/b:janc.0000043920.77446.d7

Cited by 54 publications

(26 citation statements)

References 2 publications

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“…In 1963 Sawicki et al published a review of over 50 spectrophotometric methods for the determination of nitrite 11 . In 1879, Griess proposed the reaction of sulfanilic acid with 1-naphthylamine in the presence of nitrites and sulfuric acid and proposed that it could be used for the determination of nitrites 12 . Modifications of the original Griess method have been investigated and it is still one of the most commonly employed colorimetric method for the detection of nitrites [13][14][15][16][17][18][19][20] .…”

Section: Introductionmentioning

confidence: 99%

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

et al. 2007

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An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λ max of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 µM NO 2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader.

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Section: Introductionmentioning

confidence: 99%

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

et al. 2007

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“…Prior to analysis the samples were filtered and in the case of high hydrocarbonate contents were boiled. tration in mol dm -3 , V eff the effective void volume of the porous electrode in dm 3 , and I the applied current in A. The effective void volume of the electrode can be obtained by analysing solutions of known concentrations of species and with known stoichiometry of the electrode process.…”

Section: Methodsmentioning

confidence: 99%

“…2 Nitrite is mostly detected and analysed photometrically by making use of the Griess reaction, involving the formation of a deep red-coloured azo dye as the reaction product of NO 2 -ions with sulfanilic acid and naphthyl-1-amine in acidic media. 3 These methods including also some other modified reagents enable the determination of nitrites at concentrations down to the low μg dm -3 range. Of electrochemical methods amperometry on glassy carbon electrode coated with gold 4 or modified with complexes of Fe(III) and Co(II) 5 was successfully used for nitrite determination water and orange juice samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of Nitrites in Water by In-electrode Coulometric Titration in Reticulated Vitreous Carbon Electrode

Lenghartova

Lauko

Cacho

et al. 2015

ACSi

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Nitrite in water samples was determined by in-electrode coulometric titration in a reticulated vitreous carbon (RVC) electrode of 100 ppi porosity. The sample was mixed with dilute sulphuric acid and sodium sulphate, aspirated into a flow cell with the porous electrode and nitrite was oxidised to nitrate by constant current of 5 μA at which the potential of the electrode was monitored. The limits of detection and quantification were found to be 0.4 and 1.2 μg/L, respectively. The repeatability and reproducibility were 2.2% and 2.6%, respectively. The bias at 100 μg/L were found to be 0.3%. The duration of the measurement is 2-3 min depending on the nitrite concentration. There were a few interferents only, neutral and cationic surfactants decreased and increased slightly the signal, respectively. Humic acids above 30 mg/L increased the signal by 10%. Drinking and surface water samples were analysed and the results matched well those from the photometric method.

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“…Secretion of NO by murine BV-2 cells was quantified indirectly by measuring the accumulation of its stable breakdown product, nitrite [35][36][37][38] . Briefly, 50 µL of cell culture supernatant from each well of a 24-well plate were transferred to a 96-well plate.…”

Section: Griess Assay For Nitrite Detectionmentioning

confidence: 99%

Acetaminophen metabolites p-aminophenol and AM404 inhibit microglial activation

Slattery¹,

Klegeris²

2018

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Aim: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive decline, deposits of amyloid beta and neurofibrillary tangles. Inflammation facilitated by microglia, the resident immune cells of the brain, contribute to the pathogenesis of AD. Epidemiological data indicate that nonsteroidal anti-inflammatory drugs (NSAIDs), which are cyclooxygenase (COX) inhibitors, reduce the risk of developing AD when administered over the course of two or more years. The mechanisms underlying this protective effect are unknown. Acetaminophen (paracetamol), which is not effective as an inhibitor of COX in peripheral tissues, may provide similar protection without the adverse effects of chronic NSAID use. The beneficial effects of acetaminophen have been proposed to stem from its metabolites p-aminophenol and N-arachidonoylaminophenol (AM404), of which, AM404 possesses analgesic and antipyretic properties. The goal of this study was to compare the effects of acetaminophen and its metabolites on microglial immune function and to elucidate the molecular mechanisms engaged by these compounds.Methods: Lipopolysaccharide-stimulated BV-2 murine microglia were used as models. Microglial activation was monitored by their secretion of nitric oxide.Results: P-aminophenol and AM404 suppressed nitric oxide secretion from stimulated microglia more effectively than acetaminophen through pathways that were independent of COX inhibition, cannabinoid receptor type two (CB2) inhibition, and activation of transient receptor potential cation channel subfamily V member 1 (TRPV1). Conclusion:Since AM404 has been previously demonstrated to attenuate NF-kB activation, it is likely that the protective effects of acetaminophen against adverse microglia activation are mediated by its metabolites p-aminophenol and AM404 inhibiting this transcription factor.

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The 125th Anniversary of the Griess Reagent

Cited by 54 publications

References 2 publications

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

Determination of Nitrites in Water by In-electrode Coulometric Titration in Reticulated Vitreous Carbon Electrode

Acetaminophen metabolites p-aminophenol and AM404 inhibit microglial activation

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