The 2013 special issue on stem cell biology

Li, Dangsheng

doi:10.1038/cr.2013.4

Cited by 25 publications

(12 citation statements)

References 20 publications

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“…Our demonstration of EC S-phase entry within minutes following application of static uniaxial strain also aligns well with Xiong et al’s recent finding that human umbilical vein and arterial ECs secreted Weibel-Palade bodies (WPBs) within 15 min post-equibiaxial strains of 20–50% (equal strain in both x- and y-directions) [32]. Further, Suzuma et al noted that straining retinal microvascular ECs induced rapid ERK phosphorylation and mRNA expression of vascular endothelial growth factor receptor-2 [33,34].…”

Section: Resultssupporting

confidence: 90%

Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting

et al. 2016

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Vascular endothelial cells are known to respond to a range of biochemical and time-varying mechanical cues that can promote blood vessel sprouting termed angiogenesis. It is less understood how these cells respond to sustained (i.e., static) mechanical cues such as the deformation generated by other contractile vascular cells, cues which can change with age and disease state. Here we demonstrate that static tensile strain of 10%, consistent with that exerted by contractile microvascular pericytes, can directly and rapidly induce cell cycle re-entry in growth-arrested microvascular endothelial cell monolayers. S-phase entry in response to this strain correlates with absence of nuclear p27, a cyclin-dependent kinase inhibitor. Further, this modest strain promotes sprouting of endothelial cells, suggesting a novel mechanical “angiogenic switch.” These findings suggest that static tensile strain can directly stimulate pathological angiogenesis, implying that pericyte absence or death is not necessarily required of endothelial cell re-activation.

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Section: Resultssupporting

confidence: 90%

Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting

et al. 2016

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“…TALENs have advantages over ZFNs in many aspects, such as in availability [ 16 ], specificity [ 17 ], flexibility and lower toxicity [ 18 ]. TALENs have been successfully applied for efficient gene targeting in several animal models, including rat [ 19 ], zebrafish [ 20 ], Xenopus [ 18 ], mice [ 21 ], and rabbit [ 22 ]. As of this writing, there are only three reports of KO swine produced with TALENs [ 16 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

et al. 2013

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Inbred mini-pigs are ideal organ donors for future human xenotransplantations because of their clear genetic background, high homozygosity, and high inbreeding endurance. In this study, we chose fibroblast cells from a highly inbred pig line called Banna mini-pig inbred line (BMI) as donor nuclei for nuclear transfer, combining with transcription activator-like effector nucleases (TALENs) and successfully generated α-1,3-galactosyltransferase (GGTA1) gene biallelic knockout (KO) pigs. To validate the efficiency of TALEN vectors, in vitro-transcribed TALEN mRNAs were microinjected into one-cell stage parthenogenetically activated porcine embryos. The efficiency of indel mutations at the GGTA1-targeting loci was as high as 73.1% (19/26) among the parthenogenetic blastocysts. TALENs were co-transfected into porcine fetal fibroblasts of BMI with a plasmid containing neomycin gene. The targeting efficiency reached 89.5% (187/209) among the survived cell clones after a 10 d selection. More remarkably 27.8% (58/209) of colonies were biallelic KO. Five fibroblast cell lines with biallelic KO were chosen as nuclear donors for somatic cell nuclear transfer (SCNT). Three miniature piglets with biallelic mutations of the GGTA1 gene were achieved. Gal epitopes on the surface of cells from all the three biallelic KO piglets were completely absent. The fibroblasts from the GGTA1 null piglets were more resistant to lysis by pooled complement-preserved normal human serum than those from wild-type pigs. These results indicate that a combination of TALENs technology with SCNT can generate biallelic KO pigs directly with high efficiency. The GGTA1 null piglets with inbred features created in this study can provide a new organ source for xenotransplantation research.

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“…Stem‐cell therapy has emerged as an exciting new area of medicine and surgery. Plasticity of progenitor cells has resulted in positive remodelling and regeneration of viable tissues in liver, brain, heart and other organ systems . Among the many sources of adult stem cells, CB‐derived USSCs have shown particular promise .…”

Section: Discussionmentioning

confidence: 99%

Enhancing ex vivo expansion of cord blood‐derived unrestricted somatic stem cells for clinical applications

et al. 2013

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The 2013 special issue on stem cell biology

Cited by 25 publications

References 20 publications

Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting

Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting

Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs

Enhancing ex vivo expansion of cord blood‐derived unrestricted somatic stem cells for clinical applications

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