The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease

Fukunaga, Masao; Yanagihara, Yasutake; Sohnaka, Masako

doi:10.1099/00221287-138-5-871

Cited by 28 publications

(15 citation statements)

References 19 publications

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“…Amplification of IGS and ospC sequences suggested that this borrelia was a member of the LB group, since these sequences are only present in B. burgdorferi s.l. (Fukunaga et al, 1992; Schwartz et al, 1992; Gazumyan et al, 1994; Margos et al, 2008; Rudenko et al, 2011; Stanek and Reiter, 2011; Margos et al, 2011). With regard to the housekeeping genes used in MLST, sequences obtained from the spirochete cultured from tick 5 ( B. chilensis VA1), were identical to those obtained from tick 5 itself (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Borrelia chilensis, a new member of theBorrelia burgdorferisensu lato complex that extends the range of this genospecies in theSouthernHemisphere

Ivanova

Tomova

González‐Acuña

et al. 2013

Environmental Microbiology

112

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Summary Borrelia burgdorferi sensu lato (s.l.), transmitted by Ixodes spp. ticks, is the causative agent of Lyme disease. Although Ixodes spp. ticks are distributed in both Northern and Southern Hemispheres, evidence for the presence of B. burgdorferi s.l. in South America apart from Uruguay is lacking. We now report the presence of culturable spirochetes with flat-wave morphology and borrelial DNA in endemic Ixodes stilesi ticks collected in Chile from environmental vegetation and long-tailed rice rats (Oligoryzomys longicaudatus). Cultured spirochetes and borrelial DNA in ticks were characterized by multilocus sequence typing and by sequencing five other loci (16S and 23S ribosomal genes, 5S-23S intergenic spacer, flaB, ospC). Phylogenetic analysis placed this spirochete as a new genospecies within the Lyme borreliosis group. Its plasmid profile determined by PCR and pulsed-field gel electrophoresis differed from that of B. burgdorferi B31A3. We propose naming this new South American member of the Lyme borreliosis group Borrelia chilensis VA1, in honor of its country of origin.

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Section: Resultsmentioning

confidence: 99%

Borrelia chilensis, a new member of theBorrelia burgdorferisensu lato complex that extends the range of this genospecies in theSouthernHemisphere

Ivanova

Tomova

González‐Acuña

et al. 2013

Environmental Microbiology

112

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“…The rrf (5S) and rrl (23S) genes were co-located in all three B. pilosicoli genomes, with the rrs (16S) gene located approximately 645 Kb, 679 Kb and 773 Kb from the other rRNA genes in the 95/1000, B2904 and WesB genomes, respectively. This rRNA gene organisation has been considered a distinguishing feature of Brachyspira species [44], since other spirochaetes typically have differing copy numbers and organisations [45,46]; however, similar arrangements to Brachyspira have been detected in the spirochaete Borrelia burgdorferi [47]. Situated between the rrs gene and rrf rrl cluster, which are either side of the oriC , was the tmRNA ( ssrA , 10Sa RNA) gene and nine of the total 34 tRNAs that were otherwise dispersed throughout the genome (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of Brachyspira pilosicoli strains: genome rearrangements, reductions and correlation of genetic compliment with phenotypic diversity

et al. 2012

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BackgroundThe anaerobic spirochaete Brachyspira pilosicoli causes enteric disease in avian, porcine and human hosts, amongst others. To date, the only available genome sequence of B. pilosicoli is that of strain 95/1000, a porcine isolate. In the first intra-species genome comparison within the Brachyspira genus, we report the whole genome sequence of B. pilosicoli B2904, an avian isolate, the incomplete genome sequence of B. pilosicoli WesB, a human isolate, and the comparisons with B. pilosicoli 95/1000. We also draw on incomplete genome sequences from three other Brachyspira species. Finally we report the first application of the high-throughput Biolog phenotype screening tool on the B. pilosicoli strains for detailed comparisons between genotype and phenotype.ResultsFeature and sequence genome comparisons revealed a high degree of similarity between the three B. pilosicoli strains, although the genomes of B2904 and WesB were larger than that of 95/1000 (~2,765, 2.890 and 2.596 Mb, respectively). Genome rearrangements were observed which correlated largely with the positions of mobile genetic elements. Through comparison of the B2904 and WesB genomes with the 95/1000 genome, features that we propose are non-essential due to their absence from 95/1000 include a peptidase, glycine reductase complex components and transposases. Novel bacteriophages were detected in the newly-sequenced genomes, which appeared to have involvement in intra- and inter-species horizontal gene transfer. Phenotypic differences predicted from genome analysis, such as the lack of genes for glucuronate catabolism in 95/1000, were confirmed by phenotyping.ConclusionsThe availability of multiple B. pilosicoli genome sequences has allowed us to demonstrate the substantial genomic variation that exists between these strains, and provides an insight into genetic events that are shaping the species. In addition, phenotype screening allowed determination of how genotypic differences translated to phenotype. Further application of such comparisons will improve understanding of the metabolic capabilities of Brachyspira species.

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“…Resulting recombinant DNAs were then introduced into competent cells of Escherichia coli HB101 (Takara Shuzo, Kyoto, Japan) as described previously (13). The double-stranded plasmid DNA was extracted and purified as described previously (18). DNA sequencing was performed by the dideoxy chain termination method with an autoread sequencing kit in an automated sequencer (13).…”

Section: Methodsmentioning

confidence: 99%

Characterization of spirochetes isolated from ticks (Ixodes tanuki, Ixodes turdus, and Ixodes columnae) and comparison of the sequences with those of Borrelia burgdorferi sensu lato strains

Fukunaga

Hamase

Okada

et al. 1996

Appl Environ Microbiol

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Ixodes persulcatus serves as a tick vector for Borrelia garinii and Borrelia afzelii in Japan; however, unidentified spirochetes have been isolated from other species of ticks. In this study, 13 isolates from ticks (6 from Ixodes tanuki, 6 from Ixodes turdus, and 1 from Ixodes columnae) and 3 isolates from voles (Clethrionomys rufocanus) were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rRNA gene restriction fragment length polymorphism, partial sequencing of the outer surface protein C (OspC) gene, whole DNA-DNA hybridization, and 16S rRNA gene sequence comparison. All of the results revealed that these Borrelia strains clearly represent at least two new species. A third is also likely, although additional strains have to be isolated and characterized before a separate species is designated. We designated all isolates of I. tanuki and C. rufocanus as group Hk501 and all isolates of I. turdus as group Ya501. Phylogenetic analysis based on 16S rRNA gene sequences distinguished these Borrelia strains from those belonging to hitherto known Borrelia species. Furthermore, the genomic groups, each with its own tick vectors with enzootic cycles, were quite different from each other and also from those of Lyme disease Borrelia species known to occur in Japan. The results of 16S rRNA gene sequence comparison suggest that the strain Am501 from I. columnae is related to group Hk501, although its level of DNA relatedness is less than 70%.

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The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease

Cited by 28 publications

References 19 publications

Borrelia chilensis, a new member of theBorrelia burgdorferisensu lato complex that extends the range of this genospecies in theSouthernHemisphere

Borrelia chilensis, a new member of theBorrelia burgdorferisensu lato complex that extends the range of this genospecies in theSouthernHemisphere

Comparative genomics of Brachyspira pilosicoli strains: genome rearrangements, reductions and correlation of genetic compliment with phenotypic diversity

Characterization of spirochetes isolated from ticks (Ixodes tanuki, Ixodes turdus, and Ixodes columnae) and comparison of the sequences with those of Borrelia burgdorferi sensu lato strains

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