The 3-dimensional printing for dental tissue regeneration: the state of the art and future challenges

Zhao, Fengxiao; Zhang, Zhijun; Guo, Weihua

doi:10.3389/fbioe.2024.1356580

Front. Bioeng. Biotechnol.

2024

DOI: 10.3389/fbioe.2024.1356580

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The 3-dimensional printing for dental tissue regeneration: the state of the art and future challenges

Fengxiao Zhao,

Zhijun Zhang,

Weihua Guo

Abstract: Tooth loss or damage poses great threaten to oral and general health. While contemporary clinical treatments have enabled tooth restoration to a certain extent, achieving functional tooth regeneration remains a challenging task due to the intricate and hierarchically organized architecture of teeth. The past few decades have seen a rapid development of three-dimensional (3D) printing technology, which has provided new breakthroughs in the field of tissue engineering and regenerative dentistry. This review outl… Show more

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Cited by 4 publications

References 149 publications

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Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances

Weżgowiec,

Małysa,

Szlasa

et al. 2024

Front. Bioeng. Biotechnol.

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ObjectivesThe development of additive manufacturing has the potential to revolutionize the fabrication of medical devices. This technology, also known as 3D printing, offers precise, cost-effective, and personalized approaches, which could be particularly beneficial in the production of intraoral appliances. Despite its promise, research on the biocompatibility of 3D-printed intraoral devices is still limited. Our study aims to address this gap.MethodsWe examined the cytotoxicity of materials processed via three techniques commonly used for the fabrication of different intraoral appliances: 3D printing (Dental LT Clear), thermoforming (Duran adjusted with Durasplint LC), and conventional heat-curing (Villacryl H Plus). We also investigated the impact of chemical or UVC disinfection on the biocompatibility of these materials. We assessed the biological effects induced in human gingival fibroblasts (HGFs) through both direct contact tests (MTT and LDH assays) and extract tests (PrestoBlue, DCF, and cell death type assays). Additionally, we observed changes in cellular morphology and migration rate under an inverted light microscope. The surface roughness of materials was evaluated using contact profilometry. Statistical analysis was conducted using two-way analysis of variance.ResultsOur findings suggest that all three fabrication techniques induced a slight cytotoxic effect in HGFs, as evidenced by both direct contact and extract tests. However, these materials could be considered nontoxic according to the ISO 10993-5:2009 norm, as the decrease in metabolic activity observed was always less than 30% compared to the untreated control.ConclusionThis novel study confirms that 3D printing may be a safe alternative to conventional methods for fabricating intraoral appliances. However, further tests assessing the long-term intraoral usage are still needed.

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Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances

Weżgowiec,

Małysa,

Szlasa

et al. 2024

Front. Bioeng. Biotechnol.

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Optimizing collagen-based biomaterials for periodontal regeneration: clinical opportunities and challenges

Feng,

2024

Front. Bioeng. Biotechnol.

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Periodontal disease (PD) is a chronic inflammatory condition that affects the teeth and their supporting tissues, ultimately culminating in tooth loss. Currently, treatment modalities, such as systemic and local administration of antibiotics, serve to mitigate the progression of inflammation yet fall short in restoring the original anatomical structure and physiological function of periodontal tissues. Biocompatible material-based tissue engineering seems to be a promising therapeutic strategy for treating PD. Collagen, a component of the extracellular matrix commonly used for tissue engineering, has been regarded as a promising biogenic material for tissue regeneration owing to its high cell-activating and biocompatible properties. The structural and chemical similarities between collagen and components of the oral tissue extracellular matrix render it a promising candidate for dental regeneration. This review explored the properties of collagen and its current applications in periodontal regeneration. We also discussed the recent progression in collagen therapies and preparation techniques. The review also scrutinizes the pros and cons associated with the application of collagen-based biomaterials in PD treatment, aiming to pave the way for future applications of collagen-based biomaterials in the management of PD.

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Biomaterial Scaffolds for Periodontal Tissue Engineering

Chen,

Song,

et al. 2024

JFB

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Advanced periodontitis poses a significant threat to oral health, causing extensive damage and loss of both hard and soft periodontal tissues. While traditional therapies such as scaling and root planing can effectively halt the disease’s progression, they often fail to fully restore the original architecture and function of periodontal tissues due to the limited capacity for spontaneous regeneration. To address this challenge, periodontal tissue engineering has emerged as a promising approach. This technology centers on the utilization of biomaterial scaffolds, which function as three-dimensional (3D) templates or frameworks, supporting and guiding the regeneration of periodontal tissues, including the periodontal ligament, cementum, alveolar bone, and gingival tissue. These scaffolds mimic the extracellular matrix (ECM) of native periodontal tissues, aiming to foster cell attachment, proliferation, differentiation, and, ultimately, the formation of new, functional periodontal structures. Despite the inherent challenges associated with preclinical testing, the intensification of research on biomaterial scaffolds, coupled with the continuous advancement of fabrication technology, leads us to anticipate a significant expansion in their application for periodontal tissue regeneration. This review comprehensively covers the recent advancements in biomaterial scaffolds engineered specifically for periodontal tissue regeneration, aiming to provide insights into the current state of the field and potential directions for future research.

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The 3-dimensional printing for dental tissue regeneration: the state of the art and future challenges

Cited by 4 publications

References 149 publications

Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances

Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances

Optimizing collagen-based biomaterials for periodontal regeneration: clinical opportunities and challenges

Biomaterial Scaffolds for Periodontal Tissue Engineering

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