The 3D structure of the tectorial membrane determined by second-harmonic imaging microscopy

Gueta, Rachel; Tal, Eran; Silberberg, Yaron; Rousso, Itay

doi:10.1016/j.jsb.2007.03.002

Cited by 27 publications

(28 citation statements)

References 26 publications

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“…According to our model, the reason for this altered longitudinal pattern of OHC loss in Tecta C1509G/+ mice, whereby OHCs at the base are relatively spared compared to those located more apically, reflects the impact of biomechanical changes within the mutant TM. These changes are predicted to alter the pattern of OHC stereociliary stimulation along the cochlear partition (Gavara and Chadwick 2009;Gu et al 2008;Gueta et al 2006Gueta et al , 2007Gueta et al , 2008Masaki et al 2010;Meaud et al 2010;Shoelson et al 2004). As well, this may represent a shift in the tonotopic map of the cochlea secondary to altered TM and OHC stiffness gradients (Choi and Oghalai 2008;Deo and Grosh 2004;Ghaffari et al 2007;He et al 2003;Liu and Neely 2009;Masaki et al 2009;Richter et al 2007;Sfondouris et al 2008;Stasiunas et al 2009) or reduced gain of the cochlear amplifier (Oghalai 2004a).…”

Section: Discussionmentioning

confidence: 99%

Biophysical Mechanisms Underlying Outer Hair Cell Loss Associated with a Shortened Tectorial Membrane

Liu

Gao

Tao

et al. 2011

JARO

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The tectorial membrane (TM) connects to the stereociliary bundles of outer hair cells (OHCs). Humans with an autosomal dominant C1509G mutation in alpha-tectorin, a protein constituent of the TM, are born with a partial hearing loss that worsens over time. The Tecta C1509/+ transgenic mouse with the same point mutation has partial hearing loss secondary to a shortened TM that only contacts the first row of OHCs. As well, Tecta C1509G/+ mice have increased expression of the OHC electromotility protein, prestin. We sought to determine whether these changes impact OHC survival. Distortion product otoacoustic emission thresholds in a quiet environment did not change to 6 months of age. However, noise exposure produced acute threshold shifts that fully recovered in Tecta +/+ mice but only partially recovered in Tecta C1509G/+ mice. While Tecta +/+ mice lost OHCs primarily at the base and within all three rows, Tecta C1509G/+ mice lost most of their OHCs in a more apical region of the cochlea and nearly completely within the first row. In order to estimate the impact of a shorter TM on the forces faced by the stereocilia within the first OHC row, both the wild type and the heterozygous conditions were simulated in a computational model. These analyses predicted that the shear force on the stereocilia is~50% higher in the heterozygous condition. We then measured electrically induced movements of the reticular lamina in situ and found that while they decreased to the noise floor in prestin null mice, they were increased by 4.58 dB in Tecta C1509G/+ mice compared to Tecta +/+ mice. The increased movements were associated with a fourfold increase in OHC death as measured by vital dye staining. Together, these findings indicate that uncoupling the TM from some OHCs leads to partial hearing loss and places the remaining coupled OHCs at higher risk. Both the mechanics of the malformed TM and the increased prestin-related movements of the organ of Corti contribute to this higher risk profile.

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Section: Discussionmentioning

confidence: 99%

Biophysical Mechanisms Underlying Outer Hair Cell Loss Associated with a Shortened Tectorial Membrane

Liu

Gao

Tao

et al. 2011

JARO

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“…It is the stiffest part of the TM and, in the basal turn of the cochlea, is the only region of the TM that becomes increasingly stiffer with increasing frequency place on the BM (8,40). Gueta et al (40) presumed that the place-dependent stiffness gradient of this zone was to facilitate energy transfer with the OHC hair bundles, whose stiffness also increases with increasing distance from the apex of the cochlea (41,42). It would appear, according to the findings reported here, that any loss of SSM, especially in the hairbundle attachment zone of the TM, is associated with a loss of the frequency-dependent stiffness of the material properties of the TM.…”

Section: In Vitro Traveling Wave Propagation Is Disrupted In All Thrementioning

confidence: 99%

Modified Protein Expression in the Tectorial Membrane of the Cochlea Reveals Roles for the Striated Sheet Matrix

Jones

Elliott

Russell

et al. 2015

Biophysical Journal

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The tectorial membrane (TM) of the mammalian cochlea is a complex extracellular matrix which, in response to acoustic stimulation, displaces the hair bundles of outer hair cells (OHCs), thereby initiating sensory transduction and amplification. Here, using TM segments from the basal, high-frequency region of the cochleae of genetically modified mice (including models of human hereditary deafness) with missing or modified TM proteins, we demonstrate that frequency-dependent stiffening is associated with the striated sheet matrix (SSM). Frequency-dependent stiffening largely disappeared in all three TM mutations studied where the SSM was absent either entirely or at least from the stiffest part of the TM overlying the OHCs. In all three TM mutations, dissipation of energy is decreased at low (<8 kHz) and increased at high (>8 kHz) stimulus frequencies. The SSM is composed of polypeptides carrying fixed charges, and electrostatic interaction between them may account for frequency-dependent stiffness changes in the material properties of the TM. Through comparison with previous in vivo measurements, it is proposed that implementation of frequency-dependent stiffening of the TM in the OHC attachment region facilitates interaction among tones, backward transmission of energy, and amplification in the cochlea.

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“…Collagen filaments at the base of the cochlea are thinner and positioned parallel to one another and to the plane of the TM surface. Fibers at the apex are thicker and have been suggested to tilt at an angle with respect to the TM surface (Gueta et al, 2007). The overall structure of the TM varies along the cochlear axis, with the TM at the base being narrow and thin and at the apex, wider and thicker (Gueta et al, 2007).…”

Section: Tectorial Membranementioning

confidence: 99%

Development of tonotopy in the auditory periphery

Mann

Kelley

2011

Hearing Research

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The 3D structure of the tectorial membrane determined by second-harmonic imaging microscopy

Cited by 27 publications

References 26 publications

Biophysical Mechanisms Underlying Outer Hair Cell Loss Associated with a Shortened Tectorial Membrane

Biophysical Mechanisms Underlying Outer Hair Cell Loss Associated with a Shortened Tectorial Membrane

Modified Protein Expression in the Tectorial Membrane of the Cochlea Reveals Roles for the Striated Sheet Matrix

Development of tonotopy in the auditory periphery

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