The 60‐kDa precursor to the dithiothreitol‐sensitive tetrameric protease of spinach thylakoids: structural similarities between the protease and polyphenol oxidase

Kuwabara, Tomohiko

doi:10.1016/0014-5793(95)00911-r

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…A curious finding on the possible ability of PPO to act as a protease has been reported (Sokolenko et al, 1995;Kuwabara, 1995;Kuwabara et al, 1997;Kuwabara and Katoh, 1990). According to these reports a protein said to be identical with PPO in structure and properties can under certain conditions oxidatively degrade a low molecular weight protein located in chloroplasts.…”

Section: Plant Ppomentioning

confidence: 96%

Polyphenol oxidases in plants and fungi: Going places? A review

2006

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Section: Plant Ppomentioning

confidence: 96%

Polyphenol oxidases in plants and fungi: Going places? A review

2006

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“…In addition, it includes two isoforms of a plastid polyphenol oxidase (1748 and 1754) that potentially regulates photosynthesis (Dry and Robinson, 1994;Kuwabara, 1995). Another transcript linked to chloroplast metabolism encodes a plastid plastocyanin (TC65998) whose role may be to facilitate electron transport between the cytochrome b 6 f complex and PSI (Sugawara et al, 1999).…”

Section: Integration Of Data Sets (Hypothesis-free Approach)mentioning

confidence: 99%

Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Zamboni

Carli²,

Guzzo³

et al. 2010

Plant Physiology

150

135

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The analysis of grapevine (Vitis vinifera) berries at the transcriptomic, proteomic, and metabolomic levels can provide great insight into the molecular events underlying berry development and postharvest drying (withering). However, the large and very different data sets produced by such investigations are difficult to integrate. Here, we report the identification of putative stage-specific biomarkers for berry development and withering and, to our knowledge, the first integrated systems-level study of these processes. Transcriptomic, proteomic, and metabolomic data were integrated using two different strategies, one hypothesis free and the other hypothesis driven. A multistep hypothesis-free approach was applied to data from four developmental stages and three withering intervals, with integration achieved using a hierarchical clustering strategy based on the multivariate bidirectional orthogonal projections to latent structures technique. This identified stage-specific functional networks of linked transcripts, proteins, and metabolites, providing important insights into the key molecular processes that determine the quality characteristics of wine. The hypothesis-driven approach was used to integrate data from three withering intervals, starting with subdata sets of transcripts, proteins, and metabolites. We identified transcripts and proteins that were modulated during withering as well as specific classes of metabolites that accumulated at the same time and used these to select subdata sets of variables. The multivariate bidirectional orthogonal projections to latent structures technique was then used to integrate the subdata sets, identifying variables representing selected molecular processes that take place specifically during berry withering. The impact of this holistic approach on our knowledge of grapevine berry development and withering is discussed.

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“…Spinach thylakoid PPO has also been correlated with dithiothreitol-sensitive proteases (39 and 60 kDa) which were extracted with 1 M NaCl from spinach PSII membranes (Kuwabara & Hashimoto, 1990;Kuwabara, 1995). These proteins had the same N-terminal sequence, APILPDVEK-, as the above-mentioned 64 kDa protein.…”

mentioning

confidence: 99%

“…At present 27 polypeptides are identified as either PSII subunits or proteins associated with the PSII complex (Andersson & Styring, 1991;Irrgang et al, 1993;Kuwabara & Hashimoto, 1990;Kuwabara, 1995;Zhang et al, 1995). Only two of the 27 proteins, the heterodimer of the D1 and D2 subunits which forms the reaction center, bind all of the redox components that are necessary for primary charge separation and water oxidation.…”

mentioning

confidence: 99%

Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase

Sheptovitsky

Brudvig

1996

Biochemistry

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Photosystem II (PSII) membranes exhibit catalase and polyphenol oxidase (PPO) activities. Mild heat treatment of PSII membranes for 90 min at 30 degrees C releases most of these enzyme activities into the supernatant, accompanied by a 7-fold activation of PPO. In contrast, mild heat treatment of thylakoid membranes does not release significant amounts of either activity, indicating that both enzymes are bound to the luminal surface of the thylakoid membrane. The heat-released PSII membrane-associated catalase and PPO have been purified and characterized. Catalase activity was correlated with a 63 kDa polypeptide which was purified by batch adsorption to anion-exchange beads followed by gel filtration. The PSII membrane-associated catalase is unstable in solution, probably due to irreversible aggregation. The enzyme was characterized in terms of molecular and subunit size, amino-acid composition, UV-visible absorption, heme content, pH optimum, inhibitor sensitivity, and K(m) value for H2O2. Its properties indicate that the PSII membrane-associated catalase is a luminal thylakoid membrane-bound heme enzyme that has not been identified previously. The residual catalase activity of PSII membranes after mild heat treatment is irreversibly inhibited with 3-amino-1,2,4-triazole, a specific inhibitor of heme catalases, without inhibition of O2-evolution activity. This result indicates that little, if any, of the catalase activity from PSII membranes in the dark is catalyzed by the O2-evolving center of PSII. PPO activity was correlated with a 48 kDa polypeptide. However, the 48 kDa polypeptide and another heat-released polypeptide of 72 kDa have the same N-terminal sequence, which is also identical to that of a known 64 kDa protein [Hind, G., Marshak, D. R., & Coughlan, S. J. (1995) Biochemistry 34, 8157-8164]. During heat treatment of PSII membranes and further manipulations it was found that the 72 kDa polypeptide was largely converted into the 48 kDa polypeptide. Thus, the 72 kDa polypeptide appears to be a latent precursor of the active 48 kDa PPO. The PSII membrane-associated PPO was purified by anion-exchange chromatography and was characterized in terms of substrate specificity, pH optimum, inhibitor sensitivity and native molecular weight. The heat-released PPO appears to be identical to the enzyme previously isolated from spinach thylakoid membranes [Golbeck, J. H., & Cammarata, K. V. (1981) Plant Physiol. 67, 977-984].

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The 60‐kDa precursor to the dithiothreitol‐sensitive tetrameric protease of spinach thylakoids: structural similarities between the protease and polyphenol oxidase

Cited by 7 publications

References 21 publications

Polyphenol oxidases in plants and fungi: Going places? A review

Polyphenol oxidases in plants and fungi: Going places? A review

Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase

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