The AbaR antibiotic resistance islands found in Acinetobacter baumannii global clone 1 – Structure, origin and evolution

Hamidian, Mohammad; Hall, Ruth M.

doi:10.1016/j.drup.2018.10.003

Cited by 82 publications

(98 citation statements)

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“…The class 1 integron found in A144 and A155 had the genetic platform IS26-Tn21-intI1-aac(6 )-Ib-qacE 1-sul1-orf5. The presence of aac(6 )-Ib within the variable region of a class 1 integron in an AbaR0-type GI is unusual since it commonly contains the array aacC1-orfP-orfP-orfQ-aadA1 (Kochar et al, 2012;Hamidian and Hall, 2018b). Downstream of orf5 we found the genes resX and trbI, as previously described (Kochar et al, 2012).…”

Section: Genomic Analysis and Maintenance Along Time Of The Abar0-typsupporting

confidence: 74%

“…A144 and A155 genomes contained an AbaR0-type backbone GI, with the typical complete intI1 gene . The remaining GC1 Group 1 strains from lineage 1 carried AbaR0 or AbaR3-types GIs (Hamidian et al, 2014;Holt et al, 2016;Hamidian and Hall, 2018b). Many of them showed deletions in their structure caused by IS26 (Data not shown), probably due to either recombination events between duplicate copies of sul1 or Tn6018, or by gene cassette addition or replacement as previously described (Holt et al, 2016;Hamidian and Hall, 2018b).…”

Section: Genomic Analysis and Maintenance Along Time Of The Abar0-typsupporting

confidence: 54%

“…The aim of this work was to examine the role of the accessory genome of the high-risk clone GC1 across time and continents from genomic and functional approaches. Although previous comparative genomic studies evidenced genetic variability across all A. baumannii strains (Adams et al, 2008;Vallenet et al, 2008;Di Nocera et al, 2011;Sahl et al, 2013;Touchon et al, 2014;Holt et al, 2019;Meumann et al, 2019), no data, excluding evolution to antimicrobial resistance (Karah et al, 2017;Hamidian and Hall, 2018b;Holt et al, 2019), is focused on the features of the accessory genome of prevalent clones. Interestingly, two patterns of preservation of the accessory genome within GC1 strains regardless their site or time of isolation were found: (i) "sedentary" modules such as two novel regions of genome plasticity, the AbaR GI in lineage 1, and even a CRISPR-Cas type-If system located in the same loci; and (ii) a "mobile" module as the case of the putative prophage YMC/09/02/B1251_ABA_BP shared by all 106 GC1 genomes which showed high genomic plasticity evidenced by intrinsic microevolution as well as mobility to different insertion sites amongst GC1's chromosomes.…”

Section: Introductionmentioning

confidence: 99%

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Crucial Role of the Accessory Genome in the Evolutionary Trajectory of Acinetobacter baumannii Global Clone 1

et al. 2020

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Frontiers in Microbiology | www.frontiersin.org March 2020 | Volume 11 | Article 342 Álvarez et al. Accessory Genome in IC1versus A118 (ST 404/ND) without antimicrobial pressure suggested a higher ability of GC1 to grow over a clone with sporadic behavior which explains, from an ecological perspective, the global achievement of this successful pandemic clone in the hospital habitat. Together, these data suggest an essential role of still unknown properties of "mobile" and "sedentary" accessory genome that is preserved over time under different antibiotic or stress conditions.

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Section: Genomic Analysis and Maintenance Along Time Of The Abar0-typsupporting

confidence: 74%

Section: Genomic Analysis and Maintenance Along Time Of The Abar0-typsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Crucial Role of the Accessory Genome in the Evolutionary Trajectory of Acinetobacter baumannii Global Clone 1

et al. 2020

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“…The data show that the AbaR-inactivated comM gene, once cured of AbaR, is still functional. This suggests that it has not been affected by mutational drifts, confirming that acquisition of AbaR occurred relatively recently (22).…”

Section: Curing Of Abar and Repair Of The Comm Gene Results In Increassupporting

confidence: 57%

“…This strain bears a 19.7 kbplong AbaR11 inserted in the comM gene with 19 predicted genes corresponding to five modules found in other AbaRs ( Fig. 2A) (9,21,22). In a previous study, we identified the strain AB5075 as naturally transformable (16).…”

Section: Curing Abar In a Baumannii Using A Scarless Genome Editing mentioning

confidence: 94%

Scarless removal of large resistance island AbaR results in antibiotic susceptibility and increased natural transformability inAcinetobacter baumannii

Godeux

Svedholm

Lupo

et al. 2020

Preprint

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With a great diversity in gene composition including multiple putative antibiotic-resistance genes, AbaR islands are potential contributors to multi-drug resistance in Acinetobacter baumannii. However, the effective contribution of AbaR to antibiotic resistance and bacterial physiology remains elusive. To address this, we exploited natural transformation to accurately remove AbaR islands and restore the integrity of their insertion site. We performed this genetic modification in two recent A. baumannii clinical strains: the strain AB5075 and the nosocomial strain AYE which carry AbaR11 and AbaR1 islands of 19.7 kbp and 86.2 kbp, respectively. Antibiotic susceptibilities were then compared between the parental strains and their AbaR-cured derivatives. As anticipated by the predicted function of the ORF of this island, the antibiotic resistance profiles were identical between the wild-type and the AbaR11cured strain. In contrast, AbaR1 carries 25 ORFs with a predicted resistance to several classes of antibiotics and the AbaR1-cured derivative showed restored susceptibility to multiple classes of antibiotics. Moreover, curing of AbaRs restored high levels of natural transformability. Indeed, most AbaR islands are inserted into the comM gene involved in natural transformation. Our data indicate that AbaR insertion effectively inactivates comM and that the restored comM is functional. Curing of AbaR consistently resulted in highly transformable, and therefore, easily genetically tractable strains.

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Acinetobacter

Nemec

2022

Bergey's Manual of Systematics of Archaea and Bacteria

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A.ci.ne.to.bac'ter. Gr. masc. adj. akinetos unable to move; N.L. masc. adj. bacter , a rod; N.L. masc. n. Acinetobacter , a non‐motile rod. Proteobacteria / Gammaproteobacteria / Pseudomonadales / Moraxellaceae / Acinetobacter Rods 0.9–1.6 × 1.5–2.5 μm, becoming spherical in the stationary phase of growth. Colonies are generally nonpigmented and are mucoid when the cells are encapsulated. Cells commonly occur in pairs. Do not form spores. Gram‐stain‐negative. Flagella are absent, and swimming motility does not occur, but cells display twitching motility associated with fimbriae. Strictly aerobic. Oxidase‐negative. Catalase‐positive. Mesophilic. Most strains do not reduce nitrate to nitrite in conventional assays. Grow well on complex media. Most strains grow in defined media containing a single carbon and energy source (most commonly acetate), using ammonium or nitrate salts or one of several common amino acids, as a source of nitrogen. The genome consists of a single circular chromosome sized 2.6−4.7 Mb and a strain‐dependent set of plasmid replicons. Widespread in nature, inhabiting diverse soil and water ecosystems and being associated with plants and animals. Some species reside on the human skin and mucosa. Several species can cause nosocomial infections; strains involved are often resistant to multiple antibiotics and can give rise to epidemics. In 2021, the genus included 73 species with correct names. DNA G + C content (mol%) : 34.9−49.6 (median: 40.2). Type species : Acinetobacter calcoaceticus Baumann et al. 1968b AL (basonym: Micrococcus calcoaceticus Beijerinck 1911) emend. Bouvet and Grimont 1986.

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The AbaR antibiotic resistance islands found in Acinetobacter baumannii global clone 1 – Structure, origin and evolution

Cited by 82 publications

References 81 publications

Crucial Role of the Accessory Genome in the Evolutionary Trajectory of Acinetobacter baumannii Global Clone 1

Crucial Role of the Accessory Genome in the Evolutionary Trajectory of Acinetobacter baumannii Global Clone 1

Scarless removal of large resistance island AbaR results in antibiotic susceptibility and increased natural transformability inAcinetobacter baumannii

Acinetobacter

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