The ABC transporter Snu and the extracellular protein Snsl cooperate in the formation of the lipid-based inward and outward barrier in the skin of Drosophila

Zuber, Renata; Norum, Michaela; Wang, Yiwen; Oehl, Kathrin; Gehring, Nicole; Accardi, Davide; S, Bartozsewski; Berger, Jürgen; Flötenmeyer, Matthias; Moussian, Bernard

doi:10.1016/j.ejcb.2017.12.003

Cited by 47 publications

(63 citation statements)

References 35 publications

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“…To further inspect cuticle barrier integrity, we performed a dye penetration assay that we had developed recently (Wang et al, 2017a; Wang et al, 2016). Incubation of wild-type, slf and alas mutant ready-to-hatch embryos with bromophenol blue does not result in dye uptake, while snurstorr snarlik ( snsl ) mutant animals with a defective envelope (Zuber et al, 2018) do so (Fig. S2).…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we had shown that a heme-dependent pathway is required to generate a dityrosine-based waterproof matrix within the cuticle of the D. melanogaster larva (Shaik et al, 2012). Recently, we reported on the role of the ABC transporter Snustorr (Snu) and the extracellular protein Snustorr-snarlik (Snsl) in the construction of an envelope-based anti-penetration and anit-transpiration barrier in D. melanogaster (Zuber et al, 2017). In the present work, we propose that the C-type lectin Slf cooperates with the heme-biosynthesis pathway to stabilise the distribution of the cuticle dityrosinylated proteins, exemplified by Resilin.…”

Section: Discussionmentioning

confidence: 99%

“…Classically, the outermost cuticle layer called envelope has been considered to be the bona fide desiccation barrier. In a recent work, we demonstrated that the extracellular protein Snsl and the ABC transporter Snu contribute to the establishment of the envelope in turn ensuring desiccation as well as penetration resistance (Zuber et al, 2017). The function of Snu is obviously conserved in other insects (Broehan et al, 2013; Yu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Stained embryos were viewed on a Zeiss Axiophot, Olympus flowview FV1000, Zeiss LSM 710 or 880 confocal microscope. For permeability experiments, embryos were dechorionated, devitellinized and incubated in bromophenol blue solution following the protocol described in (Zuber et al, 2018). For electron microscopy, specimens were prepared following the protocol described in Moussian and Schwarz (Moussian and Schwarz, 2010).…”

Section: Methodsmentioning

confidence: 99%

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The C-type lectin Schlaff ensures epidermal barrier compactness inDrosophila

Zuber

Shaik

Meyer

et al. 2018

Preprint

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20The stability of extracellular matrices is in general ensured by cross-linking of its 21 components. Previously, we had shown that the integrity of the layered Drosophila 22 cuticle relies on the presence of a covalent cuticular dityrosine network. Production 23 and composition of this structure remained unstudied. In this work, we present our 24 analyses of the schlaff (slf) gene coding for a C-type lectin that is needed for the 25 adhesion between the horizontal cuticle layers. The Slf protein mainly localizes 26 between the two layers called epicuticle and procuticle that separate from each other 27 when the function of Slf is reduced or eliminated paralleling the phenotype of a 28 cuticle with reduced extracellular dityrosine. Localisation of the dityrosinylated protein 29

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The C-type lectin Schlaff ensures epidermal barrier compactness inDrosophila

Zuber

Shaik

Meyer

et al. 2018

Preprint

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“…The innermost procuticle consists of a chitin‐protein matrix, the middle epicuticle is composed of proteins, and the outermost envelope is made up of lipids and proteins. While the constituents of the epicuticle are unknown, the molecular players implementing the procuticle and the envelope are to some extent well studied in various insect species (Chaudhari et al, ; Moussian et al, ; Moussian, Schwarz, Bartoszewski, & Nusslein‐Volhard, 2005; Moussian, Soding, Schwarz, & Nusslein‐Volhard, 2005; Moussian et al, ; Pesch, Riedel, & Behr, ; Pesch, Riedel, & Behr, ; R. Yu et al, ; Z. Yu et al, ; Zuber et al, ). A central enzyme of procuticle formation is the glycosyltransferase chitin synthase that is inserted in the apical plasma membrane of epidermal cells and synthesises chitin fibres that protrude into the extracellular space.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of fatty acid desaturation impairs cuticle differentiation in Drosophila melanogaster

Wang

Maier

Gehring

et al. 2019

Arch Insect Biochem Physiol

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Previously, we showed that inhibition of the activity of fatty acid desaturases (Desat) perturbs signalling of the developmental timing hormone ecdysone in the fruit fly Drosophila melanogaster. To understand the impact of this effect on cuticle differentiation, a process regulated by ecdysone, we analysed the cuticle of D. melanogaster larvae fed with the Desat inhibitor CA10556. In these larvae, the expression of most of the key cuticle genes is normal or slightly elevated at day one of CA10556 feeding. As an exception, expression of twdlM coding for a yet uncharacterised cuticle protein is completely suppressed. The cuticle of these larvae appears to be normal at the morphological level. However, these animals are sensitive to desiccation, a trait that according to our data, among others, may be associated with reduced TwdlM amounts. At day two of CA10556 feeding, expression of most of the cuticle genes tested including twdlM is suppressed. Expression of cpr47Eb coding for a chitinbinding protein is, by contrast, highly elevated suggesting that Cpr47Eb participates at a specific compensation program. Overall, the cuticle of these larvae is thinner than the cuticle of control larvae. Taken together, lipid desaturation is necessary for a coordinated deployment of a normal cuticle differentiation program. K E Y W O R D S barrier, cuticle, differentiation, epidermis, lipids Arch. Insect Biochem. Physiol. 2019;100:e21535.wileyonlinelibrary.com/journal/arch

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Xenobiotic responses of Drosophila melanogaster to insecticides with different modes of action and entry

Gao

Zang

Qiao

et al. 2022

Arch Insect Biochem Physiol

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Depending on their chemical structure, insecticides enter the insect body either through the cuticle or by ingestion (mode of entry [MoE]), and, naturally, harm or even kill insects through different mechanisms (modes of action). In parallel, they trigger a systemic detoxification response, especially by activation of detoxification gene expression.We monitored the acute genetic alterations of known xenobiotic response target genes against five different insecticides with two most common MoEs (contact toxicity and stomach toxicity), found that: 1. only a few genes were detected responding to acute exposure to insecticides (LD 90 ); 2. The expression of cyp12d1 was upregulated in all experiments, except for dichlorodiphenyltrichloroethane exposure, suggesting that cyp12d1 is a general first response gene of the xenobiotic response; 3. The contact and stomach entries did not show any notable difference, both MoEs induced the response of JNK signaling pathway, possibly serving as the driver of the response of cyp12d1 and a few other genes. In conclusion, the changes in gene expression levels were relatively modest and no significant differences were found between the two MoEs, so the insecticide entry route does not seem to have an impact on the detoxification response. However,

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The ABC transporter Snu and the extracellular protein Snsl cooperate in the formation of the lipid-based inward and outward barrier in the skin of Drosophila

Cited by 47 publications

References 35 publications

The C-type lectin Schlaff ensures epidermal barrier compactness inDrosophila

The C-type lectin Schlaff ensures epidermal barrier compactness inDrosophila

Inhibition of fatty acid desaturation impairs cuticle differentiation in Drosophila melanogaster

Xenobiotic responses of Drosophila melanogaster to insecticides with different modes of action and entry

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