The abfB gene encoding the major α-L-arabinofuranosidase of Aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain

Gielkens, M.M.C.; González-Candelas, Luı́s; Sánchez-Torres, Paloma; Vondervoort, P. van de; Graaff, L.H. de; Visser, Jaap; Ramón, Daniel

doi:10.1099/13500872-145-3-735

Cited by 60 publications

(39 citation statements)

References 27 publications

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“…As mentioned above, arabinofuranosidases and endoarabinases are induced when Aspergillus is grown on sugar beet pulp whereas AXH are produced during growth on xylan (see "␣-L-Arabinofranosidases and arabinoxylan arabinofuranohydrolases" above). Expression of the genes encoding these enzymes was also observed on monomeric carbon sources, such as L-arabinose and L-arabitol (111,125,126,303,375). The expression of two arabinofuranosidase-encoding genes (abfA and abfB) and one VOL.…”

Section: Expression Of Specific Genes Responding To Different Inducersmentioning

confidence: 97%

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

Vries

Visser

2001

Microbiol Mol Biol Rev

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585

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SUMMARY Degradation of plant cell wall polysaccharides is of major importance in the food and feed, beverage, textile, and paper and pulp industries, as well as in several other industrial production processes. Enzymatic degradation of these polymers has received attention for many years and is becoming a more and more attractive alternative to chemical and mechanical processes. Over the past 15 years, much progress has been made in elucidating the structural characteristics of these polysaccharides and in characterizing the enzymes involved in their degradation and the genes of biotechnologically relevant microorganisms encoding these enzymes. The members of the fungal genus Aspergillus are commonly used for the production of polysaccharide-degrading enzymes. This genus produces a wide spectrum of cell wall-degrading enzymes, allowing not only complete degradation of the polysaccharides but also tailored modifications by using specific enzymes purified from these fungi. This review summarizes our current knowledge of the cell wall polysaccharide-degrading enzymes from aspergilli and the genes by which they are encoded.

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Section: Expression Of Specific Genes Responding To Different Inducersmentioning

confidence: 97%

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

Vries

Visser

2001

Microbiol Mol Biol Rev

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886

585

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“…Arabinanolytic genes are specifically induced when A. niger is grown on arabinan-containing substrates or the monomeric compounds L-arabinose and L-arabitol Gielkens et al, 1997Gielkens et al, , 1999Ramon et al, 1993;van der Veen et al, 1991van der Veen et al, , 1993. One of the intermediates of the L-arabinose catabolic pathway, L-arabitol, is believed to be the low-molecular-mass inducer of the system (van der .…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism

et al. 2003

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This paper describes two Aspergillus niger mutants (araA and araB) specifically disturbed in the regulation of the arabinanase system in response to the presence of L-arabinose. Expression of the three known L-arabinose-induced arabinanolytic genes, abfA, abfB and abnA, was substantially decreased or absent in the araA and araB strains compared to the wild-type when incubated in the presence of L-arabinose or L-arabitol. In addition, the intracellular activities of L-arabitol dehydrogenase and L-arabinose reductase, involved in L-arabinose catabolism, were decreased in the araA and araB strains. Finally, the data show that the gene encoding D-xylulose kinase, xkiA, is also under control of the arabinanolytic regulatory system. L-Arabitol, most likely the true inducer of the arabinanolytic and L-arabinose catabolic genes, accumulated to a high intracellular concentration in the araA and araB mutants. This indicates that the decrease of expression of the arabinanolytic genes was not due to lack of inducer accumulation. Therefore, it is proposed that the araA and araB mutations are localized in positive-acting components of the regulatory system involved in the expression of the arabinanase-encoding genes and the genes encoding the L-arabinose catabolic pathway.

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“…With respect to the regulation of xylanase gene expression, work has been carried out in arabinofuranosidases, β-xylosidases and endoxylanases, primarily in fungi such as Aspergillus and Trichoderma (12,16,22,24). In Penicillium purpurogenum, we have recently shown differences in the expression of two endoxylanases (6), which could be related to the presence of regulatory elements such as CreA (a negatively acting regulatory protein mediating carbon catabolite repression (8), XlnR (a transcriptional activator) (21), and PacC (a pH regulator) (19).…”

Section: Introductionmentioning

confidence: 99%

The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH

et al. 2004

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The expression of the acetyl xylan esterase II (axeII) gene from Penicillium purpurogenum is repressed by glucose and induced by xylan, as well as to a small degree by xylose and xylitol. This gene is expressed at neutral pH, but not under alkaline or acidic conditions, in agreement with previous findings for other xylanolytic genes of this organism. This is the first report showing pH regulation of an axe gene.

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The abfB gene encoding the major α-L-arabinofuranosidase of Aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain

Cited by 60 publications

References 27 publications

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

AspergillusEnzymes Involved in Degradation of Plant Cell Wall Polysaccharides

Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism

The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH

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