The ABI4‐RbohD/VTC2 regulatory module promotes reactive oxygen species (ROS) accumulation to decrease seed germination under salinity stress

Luo, Xiaofeng; Dai, Yujia; Zheng, Chuan; Yang, Yingzeng; Chen, Wei; Wang, Qichao; Chandrasekaran, Umashankar; Du, Junbo; Liu, Weiguo; Shu, Kai

doi:10.1111/nph.16921

Cited by 135 publications

(88 citation statements)

References 80 publications

(144 reference statements)

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“…Osmotic stress and ionic stress caused by saline-alkali stress further lead to the generation and accumulation of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals (OH − s). Luo et al (2021) addressed the mechanism of ROS production triggered by salinity. First, NaCl induces the expression of Abscisic Acid-Insensitive 4 (ABI4), which can enhance RbohD expression but repress VTC2 expression.…”

Section: Increasing Resistance To Oxidative Damage Via Antioxidant Enzymes and Antioxidantsmentioning

confidence: 99%

“…Then activated RbohD promotes ROS production while VTC2 repression impairs ROS scavenging. Therefore, this ABI4-RbohD/VTC2 regulatory module positively promotes ROS accumulation (Luo et al, 2021). Accumulated ROS will disrupt the normal physiological functions of cells, resulting in metabolic disorders.…”

Section: Increasing Resistance To Oxidative Damage Via Antioxidant Enzymes and Antioxidantsmentioning

confidence: 99%

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Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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As two coexisting abiotic stresses, salt stress and alkali stress have severely restricted the development of global agriculture. Clarifying the plant resistance mechanism and determining how to improve plant tolerance to salt stress and alkali stress have been popular research topics. At present, most related studies have focused mainly on salt stress, and salt-alkali mixed stress studies are relatively scarce. However, in nature, high concentrations of salt and high pH often occur simultaneously, and their synergistic effects can be more harmful to plant growth and development than the effects of either stress alone. Therefore, it is of great practical importance for the sustainable development of agriculture to study plant resistance mechanisms under saline-alkali mixed stress, screen new saline-alkali stress tolerance genes, and explore new plant salt-alkali tolerance strategies. Herein, we summarized how plants actively respond to saline-alkali stress through morphological adaptation, physiological adaptation and molecular regulation.

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Section: Increasing Resistance To Oxidative Damage Via Antioxidant Enzymes and Antioxidantsmentioning

confidence: 99%

Section: Increasing Resistance To Oxidative Damage Via Antioxidant Enzymes and Antioxidantsmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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“…We previously showed that ABI4 directly binds to the promoter of VTC2, inhibiting the transcription expression of VTC2, and then alleviating AsA biosynthesis [10]. ABI4 directly combines the key genes involved in ROS production and scavenging to modulate ROS metabolism during seed germination under salinity stress [38]. We found that AsA partially recovered the salt stress sensitivity of ABI4overexpressing plants, which had lower AsA content and more ROS accumulation.…”

Section: Introductionmentioning

confidence: 80%

Ascorbic Acid Modulation by ABI4 Transcriptional Repression of VTC2 in The Salt Tolerance of Arabidopsis

Kakan

et al. 2020

Preprint

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Background:Abscisic acid (ABA) plays an important role in plant abiotic stress responses, and ABA INSENSITIVE 4 (ABI4) is a pivotal transcription factor in the ABA signaling pathway. In Arabidopsis, ABI4 negatively regulates salt tolerance; however, the mechanism through which ABI4 regulates plant salt tolerance is poorly understood. Our previous study showed that ABI4 directly binds to the promoter of the VITAMIN C DEFECTIVE 2 (VTC2) gene, inhibiting the transcription of VTC2 and ascorbic acid (AsA) biosynthesis.Results: In the present study, we found that treatment with exogenous AsA could alleviate salt stress sensitivity of ABI4-overexpressing transgenic plants. The decreased AsA content and increased reactive oxygen species (ROS) levels in ABI4-overexpressing seedlings under salt treatment indicated that AsA-promoted ROS scavenging was related to ABI4-mediated salt tolerance. Gene expression analysis showed that ABI4 was induced at the early stage of salt stress, giving rise to reduced VTC2 expression. Accordingly, the abundance of the VTC2 protein decreased under the same salt stress conditions, and was absent in the ABI4 loss-of-function mutants, suggesting that the transcriptional inhibition of ABI4 on VTC2 resulted in the attenuation of VTC2 function. In addition, other encoding genes in the AsA biosynthesis and recycling pathways showed different responses to salt stress, demonstrating that AsA homeostasis is complicated under salinity stress. Conclusions: This study elucidates the negative modulation of ABI4 in salt stress tolerance through the regulation of AsA biosynthesis and ROS accumulation in plants.

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“…As different from positive effects of GA, IAA, and CTK on seed germination, ABA is a primary hormone that promotes seed dormancy under drought stress ( Davies and Jones, 1991 ). ABA exerts its negative effects on seed germination by weakening the endosperm, restricting radicle expansion, and stimulating transcriptional factors that positively regulates seed dormancy ( Graeber et al, 2010 ; Luo et al, 2021 ). The current study reported that white clover seeds and seedlings demonstrated significant increase in endogenous IAA, GA, or ABA content with marked reduction in CTK content under drought stress ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Diethyl Aminoethyl Hexanoate Priming Ameliorates Seed Germination via Involvement in Hormonal Changes, Osmotic Adjustment, and Dehydrins Accumulation in White Clover Under Drought Stress

et al. 2021

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Drought is a serious outcome of climate change reducing the productivity of forage species under arid and semi-arid conditions worldwide. Diethyl aminoethyl hexanoate (DA-6), a novel plant growth regulator, has proven to be involved in the amelioration of critical physiological functions in many agricultural crops under various abiotic stresses, but the role of the DA-6 in improving seed germination has never been investigated under drought stress. The present study was carried out to elucidate the impact of the DA-6 priming on seeds germination of white clover under drought stress. Results showed that seed priming with the DA-6 significantly mitigated the drought-induced reduction in germination percentage, germination vigor, germination index, seed vigor index, root length, shoot length, and fresh weight after 7 days of seed germination. The DA-6 significantly increased the endogenous indole-3-acetic acid, gibberellin, and cytokinin content with marked reduction in abscisic acid content in seedlings under drought stress. In addition, the DA-6 significantly accelerated starch catabolism by enhancing the activities of hydrolases contributing toward enhanced soluble sugars, proline content and ameliorated the antioxidant defense system to enhance the ability of reactive oxygen species scavenging under drought stress. Furthermore, exogenous DA-6 application significantly increased dehydrins accumulation and upregulated transcript levels of genes encoding dehydrins (SK2, Y2SK, or DHNb) during seeds germination under water deficient condition. These findings suggested that the DA-6 mediated seeds germination and drought tolerance associated with changes in endogenous phytohormones resulting in increased starch degradation, osmotic adjustment, antioxidants activity, and dehydrins accumulation during seed germination under water deficient condition.

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The ABI4‐RbohD/VTC2 regulatory module promotes reactive oxygen species (ROS) accumulation to decrease seed germination under salinity stress

Cited by 135 publications

References 80 publications

Response Mechanisms of Plants Under Saline-Alkali Stress

Response Mechanisms of Plants Under Saline-Alkali Stress

Ascorbic Acid Modulation by ABI4 Transcriptional Repression of VTC2 in The Salt Tolerance of Arabidopsis

Diethyl Aminoethyl Hexanoate Priming Ameliorates Seed Germination via Involvement in Hormonal Changes, Osmotic Adjustment, and Dehydrins Accumulation in White Clover Under Drought Stress

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