The ability of an antimicrobial agent to penetrate a biofilm is not correlated with its killing or removal efficiency

Araújo, Paula; Mergulhão, Filipe; Melo, L. F.; Simões, Manuel

doi:10.1080/08927014.2014.904294

Cited by 38 publications

(23 citation statements)

References 70 publications

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“…The ability of an antibiotic agent to penetrate a biofilm may not tell us anything about its efficacy (33), but penetration of the biofilm seems to be important for effective antimicrobials so that all cells are exposed to the antimicrobial agent.…”

Section: Resultsmentioning

confidence: 99%

Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

Davies

Fearn

Allsopp³

et al. 2017

mSphere

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Modern analytical techniques are becoming increasingly important in the life sciences; imaging mass spectrometry offers the opportunity to gain unprecedented amounts of information on the distribution of chemicals in samples—both xenobiotics and endogenous compounds. In particular, simultaneous imaging of antibiotics (and other antimicrobial compounds) and bacterium-derived metabolites in complex biological samples could be very important in the future for helping to understand how sample matrices impact the survival of bacteria under antibiotic challenge. We have shown that an imaging mass spectrometric technique, TOF-SIMS, will be potentially extremely valuable for this kind of research in the future.

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Section: Resultsmentioning

confidence: 99%

Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

Davies

Fearn

Allsopp³

et al. 2017

mSphere

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“…It is likely that the bacteria in the dual species biofilms had higher resistance to killing but a decreased resistance to removal, compared with the single species biofilms, particularly those formed by P. fluorescens. In fact, the killing and the removal of a biofilm are distinct processes (Araújo et al, 2014;Chen and Stewart, 2000).…”

Section: Resultsmentioning

confidence: 99%

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

Lemos

Gomes

Mergulhão

et al. 2015

Food and Bioproducts Processing

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The aim of this work was to assess the effectiveness of the biocide benzyldimethyldodecyl ammonium chloride (BDMDAC) on the removal of single and dual species biofilms of Bacillus cereus and Pseudomonas fluorescens formed in a rotating cylinder reactor (RCR), using AISI316 stainless steel (SS) and polymethyl methacrylate (PMMA) as adhesion surfaces. Additional tests were performed to understand the adhesion of B. cereus and P. fluorescens to the selected surfaces.Predictions of the adhesion potential according to the thermodynamic theory showed more favourable adhesion on SS than on PMMA, for both species.Thermodynamically, adhesion was more favourable for B. cereus. After BDMDAC treatment, thermodynamic adhesion ability was favoured for P. flurescens and decreased for B. cereus, mainly on PMMA. Both bacteria had negative surface charge and the exposure to BDMDAC increased the charge to less negative values. In vitro adhesion results were, for most cases, contradictory to those predicted by the thermodynamic theory. Single and dual species biofilms were formed in the RCR for 7 days. Afterwards, the biofilms were exposed to the chemical (use of BDMDAC) and to hydrodynamic stresses (use of increasing Reynolds number of agitation), alone and combined. The applications of BDMDAC or hydrodynamic stress, when applied alone, were insufficient to remove the biofilms from the surfaces. The combined effects of BDMDAC with a series of increasing Reynolds number of agitation promoted additional biofilm removal. This effect was dependent on the surface used. For PMMA, the hydrodynamic stress was more effective on the removal of BDMDAC-treated dual species biofilms. For SS, the synergy of the chemical and hydrodynamic stresses removed more B. cereus and dual species biofilms. The overall results 2 demonstrate that the species association was not advantageous in biofilm resistance to removal when compared with the single species biofilms, particularly those of P. fluorescens. In general, removal by hydrodynamic stress, alone and preceded by the BDMDAC treatment, was higher for biofilms formed on SS. However, even the combined action of BDMDAC and the exposure to a series of increasing Reynolds number of agitation were not effective to obtain biofilm-free surfaces.

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“…membrane resulting in release of intracellular constituents (Trueba et al, 2013). QACs are positively charged big molecules, having strong affinity to protein and lipids which lead to difficulty in diffusion through the matrix before reaching the deep biofilm layers (Smirnova et al, 2010).Previous reports showed that biofilmicidal efficacy on B. cereus caused by QACs were ~50% and their ability to remove biofilm were around 15% as penetration of biofilm is not necessarily accompanied with killing the embedded cells (Araújo et al, 2014). Beauchamp et al (2012) reported that quaternary ammonium chloride compounds were the least effective together with hypochlorite against E. coli O157:H7 biofilm cells, regardless of biofilm age, sanitizer concentration or exposure time however potas sium peroxymonosulfate have had better efficacy CONCLUSIONS C. jejuni biofilms buildup in poultry environment at different degrees regardless to time elapsed, biofilm on concrete was higher than galvanized wire and that of plastic.C.…”

Section: Resultsmentioning

confidence: 99%

Biofilmicidal Efficacy of Five Disinfectants against Campylobacter jejuni on Different Poultry Farm Surfaces

Laban¹,

Hamoud²

2019

Adv. Anim. Vet. Sci.

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Biofilm formation is a complex process, consists of a cord of molecular and physiological events that take place through several stages including adherence, formation of microcolonies, tridimensional structuring, and maturation. Biofilmacts as a reservoir not only for food production facilities but also for animal and poultry husbandry environment and considers a source of unceasing supplier of zoonotic and foodborne microorganisms infecting both hosts and consumers. Among these organisms, Campylobacter jejuni (C. jejuni) colonizes in bird hindgut with high counts. C. jejuni is widely considered as a main cause of gastroenteritis in poultry products consumers and has the ability to form biofilm on surfaces inside poultry farms and slaughterhouses or food contact surfaces. Previous studies detected the biofilm formation and efficacy of sanitizers on food contact surfaces, but fewer examined within and or on the farm components. This study aims to study the ability of C. jejuni to form biofilm on plastic, galvanized wire and concrete surfaces as simulating in poultry farms and evaluate the efficacy of five commercial disinfectants; Pyam ® , Klorsept ® , Calcium hypochlorite ® , PronTech ® and Virukill ®. All which used at two concentrations 0.5% and 1%. The used surfaces showed biofilm formation by different degrees. Our results revealed that Pyam ® , Klorsept ® and Ca. hypochlorite ® showed the highest logarithmic reductions of biofilm count followed by potassium peroxymonosulfate and halide NaCl combination Virukill ® while the lowest reductions were obtained using QAC (Alkyl Dimethyl Benzyl Ammonium Chloride 40% with 60 % Urea, PronTech ®). In conclusions, the use of efficient disinfectants reduces the risk of C. jejuni infections through direct and indirect routes of infection.

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The ability of an antimicrobial agent to penetrate a biofilm is not correlated with its killing or removal efficiency

Cited by 38 publications

References 70 publications

Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

The effects of surface type on the removal of Bacillus cereus and Pseudomonas fluorescens single and dual species biofilms

Biofilmicidal Efficacy of Five Disinfectants against Campylobacter jejuni on Different Poultry Farm Surfaces

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