The ability of brown-rot fungus Daedalea dickinsii to decolorize and transform methylene blue dye

Rizqi, Hamdan Dwi; Purnomo, Adi Setyo

doi:10.1007/s11274-017-2256-z

Cited by 46 publications

(58 citation statements)

References 32 publications

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“…The P. eryngii culture was maintained on a 9-cm diameter of PDA plates (Difco, UK) that had been incubated at 30 o C. Mycelium (1-cm diameter) were transferred into PDB (Difco) medium in a 100-ml Erlenmeyer flask (10 ml). The cultures were also pre-incubated for 7 days at 30 o C [36][37][38].…”

Section: Preparation Of Fungus Culturementioning

confidence: 99%

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Purnomo¹,

Maulianawati²,

Kamei³

2019

Journal of Microbiology and Biotechnology

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DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactantproducing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml (1 ml ≈ 1.44 × 10 1 3 CFU). After 7 d incubation, about 43% of the initial DDT (12.5 µM) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

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Section: Preparation Of Fungus Culturementioning

confidence: 99%

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Purnomo¹,

Maulianawati²,

Kamei³

2019

Journal of Microbiology and Biotechnology

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“…The F. pinicola NBRC 8705 (NITE Biological Resources Center (NBRC), Chiba, Japan) was cultivated as stock culture in a 9-cm diameter plates containing potato dextrose agar (PDA; Merck Darmstadt, Germany) at 30 C. A 1-cm diameter of mycelia from stock culture was inoculated into a 10 ml potato dextrose broth (PDB; Merck Darmstadt, Germany) medium in a 100-ml Erlenmeyer flask, and then was preincubated at 30 C for 7 days (Rizqi and Purnomo, 2017;Purnomo et al, 2017a;Boelan and Purnomo, 2019).…”

Section: Fungus Culture Conditionmentioning

confidence: 99%

Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation

Purnomo

Sariwati

Kamei

2020

Heliyon

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Trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) is a toxic and recalcitrant pesticide that has been greatly used to eradicate malaria mosquitos since the 1940s. However, the US Environmental Protection Agency banned and classified DDT as priority pollutants due to its negative impact on wildlife and human health. Considering its negative effects, it is necessary to develop effective methods of DDT degradation. A synergistic interaction of a consortium consisting of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii was adopted to degrade DDT. For the microbial consortia, F. pinicola was mixed with R. pickettii at 1, 3, 5, 7 and 10 ml (1 ml % 1.44 Â 10 13 CFU) in a potato dextrose broth (PDB) medium to degrade DDT throughout the seven days incubation period. The degradation of DDT by only the fungus F. pinicola was roughly 42%, while by only R. pickettii was 31%. The addition of 3 ml of R. pickettii into F. pinicola culture presented appropriate optimization for efficient DDT degradation at roughly 61%. The DDT transformation pathway by co-inoculation of F. pinicola and R. pickettii showed that DDT was converted to 1,1-dichloro-2,2-bis(4-chlorophenyl) ethane (DDD), further transformed to 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE), and then ultimately transformed to 1-chloro-2,2-bis(4chlorophenyl) ethylene (DDMU). These metabolites are less toxic than DDT. This research showed that R. picketti synergistically interacts with F. pinicola by enhancing DDT degradation.

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“…Additionally, [ 25 ] utilized Phanerochaete chrysosporium to degrade MB in agricultural residues. Moreover, [ 26 ] recorded the biotransformation of MB (100 mg/L) in potato dextrose broth by Daedalea dickinsii after 14 days.…”

Section: Introductionmentioning

confidence: 99%

Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

et al. 2021

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The progress in industrialization everyday life has led to the continuous entry of several anthropogenic compounds, including dyes, into surrounding ecosystem causing arduous concerns for human health and biosphere. Therefore, microbial degradation of dyes is considered an eco-efficient and cost-competitive alternative to physicochemical approaches. These degradative biosystems mainly depend on the utilization of nutritive co-substrates such as yeast extract peptone in conjunction with glucose. Herein, a synergestic interaction between strains of mixed-culture consortium consisting of Rhodotorula sp., Raoultella planticola; and Staphylococcus xylosus was recruited in methylene blue (MB) degradation using agro-industrial waste as an economic and nutritive co-substrate. Via statistical means such as Plackett–Burman design and central composite design, the impact of significant nutritional parameters on MB degradation was screened and optimized. Predictive modeling denoted that complete degradation of MB was achieved within 72 h at MB (200 mg/L), NaNO3 (0.525 gm/L), molasses (385 μL/L), pH (7.5) and inoculum size (18%). Assessment of degradative enzymes revealed that intracellular NADH-reductase and DCIP-reductase were key enzymes controlling degradation process by 104.52 ± 1.75 and 274.04 ± 3.37 IU/min/mg protein after 72 h of incubation. In addition, azoreductase, tyrosinase, laccase, nitrate reductase, MnP and LiP also contributed significantly to MB degradation process. Physicochemical monitoring analysis, namely UV−Visible spectrophotometry and FTIR of MB before treatment and degradation byproducts indicated deterioration of azo bond and demethylation. Moreover, the non-toxic nature of degradation byproducts was confirmed by phytotoxicity and cytotoxicity assays. Chlorella vulgaris retained its photosynthetic capability (˃ 85%) as estimated from Chlorophyll-a/b contents compared to ˃ 30% of MB-solution. However, the viability of Wi-38 and Vero cells was estimated to be 90.67% and 99.67%, respectively, upon exposure to MB-metabolites. Furthermore, an eminent employment of consortium either freely-suspended or immobilized in plain distilled water and optimized slurry in a bioaugmentation process was implemented to treat MB in artificially-contaminated municipal wastewater and industrial effluent. The results showed a corporative interaction between the consortium examined and co-existing microbiota; reflecting its compatibility and adaptability with different microbial niches in different effluents with various physicochemical contents.

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The ability of brown-rot fungus Daedalea dickinsii to decolorize and transform methylene blue dye

Cited by 46 publications

References 32 publications

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii

Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation

Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

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