The Ability of Chloroquine To Prevent Tat-Induced Cytokine Secretion by Monocytes Is Implicated in Its In Vivo Anti-Human Immunodeficiency Virus Type 1 Activity

Rayne, Fabienne; Vendeville, Agnès; Bonhoure, Anne; Beaumelle, Bruno

doi:10.1128/jvi.78.21.12054-12057.2004

Cited by 21 publications

(18 citation statements)

References 17 publications

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“…In agreement with the findings for the Tat peptide mentioned above, endosomal acidification is also necessary for the Tat protein to enter the cytoplasm 81. Interestingly, the in vivo anti‐HIV effect of the antimalaria drug chloroquine is based on its ability to neutralize endosomes and, thereby, prevent Tat from entering and affecting T cells 82. Vendeville et al used an elegant combination of cell biology and biochemistry to investigate the release of the Tat protein 81.…”

Section: Biophysics and Cell Biology Case By Casementioning

confidence: 99%

Break on through to the Other Side—Biophysics and Cell Biology Shed Light on Cell‐Penetrating Peptides

et al. 2005

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Cell-penetrating peptides (CPPs) have become widely used vectors for the cellular import of molecules in basic and applied biomedical research. Despite the broad acceptance of these molecules as molecular carriers, the details of the mode of cellular internalization and membrane permeation remain elusive. Within the last two years endocytosis has been demonstrated to be a route of uptake shared by several CPPs. These findings had a significant impact on CPP research. State-of-the-art cell biology is now required to advance the understanding of the intracellular fate of the CPP and cargo molecules. Owing to their presumed ability to cross lipid bilayers, CPPs also represent highly interesting objects of biophysical research. Numerous studies have investigated structure-activity relationships of CPPs with respect to their ability to bind to a lipid bilayer or to cross this barrier. Endocytosis route only relocates the membrane permeation from the cell surface to endocytic compartments. Therefore, biophysical experiments are key to a mechanistic molecular understanding of the cellular uptake of CPPs. However, biophysical investigations have to consider the molecular environment encountered by a peptide inside and outside a cell. In this contribution we will review biophysical and cell-biology data obtained for several prominent CPPs. Furthermore, we will summarize recent findings on the cell-penetrating characteristics of antimicrobial peptides and the antimicrobial properties of CPPs. Peptides of both groups have overlapping characteristics. Therefore, both fields may greatly benefit from each other. The review will conclude with a perspective of how biophysics and cell biology may synergize even more efficiently in the future.

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Section: Biophysics and Cell Biology Case By Casementioning

confidence: 99%

Break on through to the Other Side—Biophysics and Cell Biology Shed Light on Cell‐Penetrating Peptides

et al. 2005

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“…It is also widely used as an anti-malarial drug in regions of the world where HIV and malaria are co-existent. Chloroquine (10–25 μM) has been shown to confer a weak anti-HIV activity outside the range of physiological concentrations by disrupting the glycosylation of gp120 (Rayne et al, 2004; Naarding et al, 2007). None the less, some reports have demonstrated no beneficial effect of chloroquine and instead have shown increased HIV infection in vitro and a more severe form of disease outcome in brain with upregulation of viral replication of other neurotropic viruses (Kamya et al, 2001; Seth et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Chloroquine mediated molecular tuning of astrocytes for enhanced permissiveness to HIV infection

2008

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We report in this study that minimum productive HIV infection in astrocytes (a predominant cell type in brain and persists for the entire life) occurs through endocytosis. The lysosomotropic agent chloroquine enhanced permissiveness of astrocytes to HIV infection possibly by circumventing degradation of endosome-entrapped viral particles. In particular, chloroquine may promote establishment of a stable long term viral reservoir in astrocytes and may eventually facilitate early onset of neurological complications.

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“…The drug has also been shown to prevent the up-regulation of major histocompatibility complex (MHC) I antigens and cellular abnormalities in coxsackie B4-infected human foetal thymocytes (Brilot et al, 2004). The mechanism of inhibition for HIV may be related to some inhibitory interaction with gp120 (Romanelli et al, 2004) or inhibition of the inflammatory cytokine response to HIV (Rayne et al, 2004). This mechanism may be important in treating SARS-CoV, since the virus induces a severe inflammatory response in the lungs of patients (Yen et al, 2006).…”

Section: Severe Acute Respiratory Syndrome (Sars) Is a Life-threatenimentioning

confidence: 99%

Evaluation of Immunomodulators, Interferons and Known in Vitro SARS-CoV Inhibitors for Inhibition of SARS-Cov Replication in BALB/c Mice

Barnard

Day

Bailey

et al. 2006

Antivir Chem Chemother

208

193

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IFN-α-n3) did not significantly reduce lung virus titres in mice. Anti-inflammatory agents, chloroquine, amodiaquin and pentoxifylline, were also inactive in vivo, suggesting that although they may be useful in ameliorating the hyperinflammatory response induced by the virus infection, they will not significantly reduce the replication of the virus, the inducer of inflammatory response. Thus, anti-inflammatory agents may only be useful in treating virus lung infections if used in combination with agents that inhibit virus replication. In summary, the data suggest that induction of IFN by mismatched dsRNA or actual treatment with exogenous IFN-α can inhibit SARS-CoV replication in the lungs of mice.

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The Ability of Chloroquine To Prevent Tat-Induced Cytokine Secretion by Monocytes Is Implicated in Its In Vivo Anti-Human Immunodeficiency Virus Type 1 Activity

Cited by 21 publications

References 17 publications

Break on through to the Other Side—Biophysics and Cell Biology Shed Light on Cell‐Penetrating Peptides

Break on through to the Other Side—Biophysics and Cell Biology Shed Light on Cell‐Penetrating Peptides

Chloroquine mediated molecular tuning of astrocytes for enhanced permissiveness to HIV infection

Evaluation of Immunomodulators, Interferons and Known in Vitro SARS-CoV Inhibitors for Inhibition of SARS-Cov Replication in BALB/c Mice

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