The ability of Lactobacillus adhesin EF-Tu to interfere with pathogen adhesion

Dhanani, Akhilesh S.; Gaudana, Sandeep B.; Bagchi, Tamishraha

doi:10.1007/s00217-011-1443-7

Cited by 26 publications

(20 citation statements)

References 44 publications

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“…Competitive inhibition reflects the affinity of surface molecules on competing bacteria for the available adhesion sites on the gut epithelium . Both lactobacilli reduced adhesion of E. coli by between 40% and 50%, which is similar to our previous report using Caco‐2 cells . The similar large numbers of adherent lactobacilli and E. coli suggest that they compete for the same attachment sites.…”

Section: Discussionsupporting

confidence: 88%

Lactobacillus plantarum CS24.2 prevents Escherichia coli adhesion to HT‐29 cells and also down‐regulates enteropathogen‐induced tumor necrosis factor‐α and interleukin‐8 expression

Dhanani

Bagchi

2013

Microbiology and Immunology

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The aim of the present study was to evaluate the potential of Lactobacillus plantarum CS24.2 to antagonize Escherichia coli adhesion and modulate expression of the responses by HT-29 cells of inflammatory molecules to E. coli adhesion. Experiments were performed under different adhesion conditions and findings compared with the responses of Lactobacillus rhamnosus GG. Tests of competitive adhesion, adhesion inhibition and displacement assays were performed for lactobacilli (L. rhamnosus GG and L. plantarum CS24.2) and E. coli O26: H11 to HT-29 cells. Both the lactobacilli significantly reduced E. coli adhesion to HT-29 cells (P < 0.05). The ability of lactobacilli to modulate tumor necrosis factor-a and interleukin-8 expression was analyzed in HT-29 cells stimulated with E. coli using qRT-PCR. L. plantarum CS24.2 significantly down regulated expression of both the genes induced by E. coli in HT-29 cells at 6 hr as well as 24 hr, which was more significant than the corresponding findings for L. rhamnosus GG. The present findings suggest that L. plantarum CS24.2 inhibits pathogen adhesion to a similar extent as does the established probiotic strain L. rhamnosus GG. It may also attenuate tumor necrosis factor-a and interleukin-8 expression in HT-29 cells stimulated with E. coli.

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Section: Discussionsupporting

confidence: 88%

Lactobacillus plantarum CS24.2 prevents Escherichia coli adhesion to HT‐29 cells and also down‐regulates enteropathogen‐induced tumor necrosis factor‐α and interleukin‐8 expression

Dhanani

Bagchi

2013

Microbiology and Immunology

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“…Similar effects were also observed in our earlier report with the adhesion of enteropathogens to Caco‐2 cells in the presence of recombinant EF‐Tu (Dhanani et al . ). These show the significant role of EF‐Tu in Lactobacillus adhesion as well as enteropathogen inhibition.…”

Section: Discussionmentioning

confidence: 97%

“…plantarum CS24Á2, Lactobacillus rhamnosus CS25. These isolates were analysed for their significant probiotic properties in our earlier study that includes bile and acid tolerance, adhesion to Caco-2 cells and antimicrobial activity against test pathogens (Gaudana et al 2010;Dhanani et al 2011). Probiotic strain -Lact.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

“…The EF-Tu gene of Lact. plantarum CS24Á2 was cloned into the expression vector, pET30(a) with a C-terminal Histidine tag and expressed in E. coli BL21(DE3) as described earlier (Dhanani et al 2011). The purification of His-tagged recombinant EF-Tu was carried out by Ni-affinity chromatography using HIS-Select â Spin Columns as per the manufacturer's instructions (Sigma-Aldrich).…”

Section: Cloning Expression and Purification Of Recombinant Ef-tumentioning

confidence: 99%

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The expression of adhesin EF-Tu in response to mucin and its role in Lactobacillus adhesion and competitive inhibition of enteropathogens to mucin

Dhanani

Bagchi

2013

J Appl Microbiol

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Aims: To analyse the expression of EF-Tu in Lactobacillus strains with response to mucin exposure and its role in interfering with adhesion of enteropathogens to mucin. Methods and Results: The Lactobacillus strains were analysed for their ability to adhere to immobilized mucin in microtiter plates. Lactobacillus delbrueckii M and Lactobacillus plantarum CS24Á2 showed statistically significant adhesion to mucin, which was similar to Lactobacillus rhamnosus GG, the best binding probiotic strain. Lactobacillus rhamnosus GG, Lact. delbrueckii M, Lact. plantarum CS23 and Lact. plantarum CS24Á2 were able to effectively antagonize the adhesion of Escherichia coli and Salmonella enterica serovar Typhi to mucin. In the presence of Lactobacillus adhesin -EF-Tu, the adhesion of Lact. delbrueckii M and the strains of Lact. plantarum to mucin was significantly inhibited. Similarly, EF-Tu also reduced the adhesion of enteropathogens to mucin. Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of EF-Tu gene in the strains of Lact. plantarum and Lact. delbrueckii M when exposed to mucin for 3 h. Conclusions: The study shows the significant role of EF-Tu in lactobacilli adhesion and enteropathogens inhibition. Significance and Impact of the Study: The study suggests EF-Tu as an important factor linked to the Lactobacillus adhesion as well as enteropathogen inhibition. Lactobacillus plantarum CS23 and Lact. plantarum CS24Á2 can be used as potential probiotic strains.

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“…Although L. plantarum is not commonly observed in the human fecal microbiota, its occurrence has been reported in some studies using phenotypic and genotypic approaches (36)(37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Costa

Marcelino-Guimarães

Vilas-Bôas

et al. 2014

Appl Environ Microbiol

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e Lactobacillus plantarum has been used in human clinical trials to promote beneficial effects in the immune system, to alleviate intestinal disorders, and to reduce the risk of cardiovascular disease. It is also involved in many fermentation processes in the food industry. However, information on the fate of ingested L. plantarum is limited. In this study, 61 subjects received daily doses of fermented milk containing 2 ؋ 10 11 cells of L. plantarum Lp115 for different periods of time. The target microorganism was monitored in the fecal microbiota via quantitative PCR (qPCR). L. plantarum was detected and quantified in all of the subjects during the ingestion periods. The differences between the L. plantarum levels at time zero and during all the different ingestion periods were statistically significant (P ‫؍‬ 0.001). However, at 15 and 45 days after discontinuing supplementation, the number of lactobacilli was reduced to the baseline level (those at time zero). A longer period with L. plantarum in the diet did not result in increased levels of this bacterium in the stool, based on postconsumption evaluations (P ‫؍‬ 0.001). The qPCR method was specific and sensitive for L. plantarum quantification in such a complex microbial environment as the gastrointestinal tract.

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The ability of Lactobacillus adhesin EF-Tu to interfere with pathogen adhesion

Cited by 26 publications

References 44 publications

Lactobacillus plantarum CS24.2 prevents Escherichia coli adhesion to HT‐29 cells and also down‐regulates enteropathogen‐induced tumor necrosis factor‐α and interleukin‐8 expression

Lactobacillus plantarum CS24.2 prevents Escherichia coli adhesion to HT‐29 cells and also down‐regulates enteropathogen‐induced tumor necrosis factor‐α and interleukin‐8 expression

The expression of adhesin EF-Tu in response to mucin and its role in Lactobacillus adhesion and competitive inhibition of enteropathogens to mucin

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

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