The ability of mucoadhesive gingival patch loaded with EGCG on IL-6 and IL-10 expression in periodontitis

Lashari, Dur Muhammad; Aljunaid, Mohammed; Ridwan, Rini Devijanti; Diyatri, Indeswati; Lashari, Yasmeen; Rashad, Huda; Surboyo, Meircurius Dwi Condro

doi:10.1016/j.jobcr.2022.08.007

Cited by 6 publications

(8 citation statements)

References 16 publications

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“…10 As previously mentioned, IL-10 suppresses several key proinflammatory cytokines that are clinically validated to participate in the pathogenesis of any disease of the oral mucosa, especially periodontitis. 12 Not only stimulated the SHED-IL10, but the addition of EGCD and α-mangosteen also stimulated the SHED-LL37 concentration. LL37, a class of antimicrobial peptides, is the sole member of the human cathelicidin family, produced by many cell types, including macrophages, natural killer cells, skin epithelial cells, airways, mucosal, ocular surface, and intestine.…”

Section: Discussionmentioning

confidence: 92%

“…12,26,29 The IL-10 has an important role in periodontitis, 30 which is able to control the number of bacteria, 31 regulation of pro-inflammatory cytokine, and increase OPG and osteoclastogenesis. 32 The previous study by Aljunaid et al and Lashari et al also showed that the EGCG is able to stimulate the IL-10 expression 11,12 and exhibit the RANKL as an osteoclast marker. 10 As previously mentioned, IL-10 suppresses several key proinflammatory cytokines that are clinically validated to participate in the pathogenesis of any disease of the oral mucosa, especially periodontitis.…”

Section: Discussionmentioning

confidence: 99%

“…25 26 IL-10 mainly targets antigen-presenting cells, such as monocytes and macrophages, and inhibits the release of proinflammatory cytokines, such as TNF-α, IL-1β, IL-6, 27 28 granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. 12 26 29 The IL-10 has an important role in periodontitis, 30 which is able to control the number of bacteria, 31 regulation of pro-inflammatory cytokine, and increase OPG and osteoclastogenesis. 32 The previous study by Aljunaid et al and Lashari et al also showed that the EGCG is able to stimulate the IL-10 expression 11 12 and exhibit the RANKL as an osteoclast marker.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The finding showed that EGCG inhibits the process of alveolar bone damage through RANKL, 10 TNF-α, 11 and increases osteoprotegerin (OPG), 10 RANK, 10 11 and IL-10. 11 12 The effect is similar to α-mangosteen, which is able to stimulate some growth factors like tumor growth factor-β, 13 and TRAP5b 14 and also inhibit the growth of etiological bacteria 15 16 and RUNX2. 14…”

Section: Introductionmentioning

confidence: 92%

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The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

et al. 2023

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Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecules from SHED, namely cytokines, chemokines, and growth factors. The metabolite can be used in various regenerative therapy based on cell-free immunomodulatory potential effects, like interleukin 10 (IL-10) and LL37. This molecule can stimulate with epigallocatechin gallate (EGCG) and α-mangosteen and has been proven to have anti-inflammatory and antibacterial effects. This study aimed to identify the effect of EGCG and α-mangosteen to SHED metabolite, called SHED-IL10 and SHED-LL37, from six passages to obtain the optimum stimulation and able to use as periodontitis regeneration treatment. Materials and Methods The six different passages of SHED were prepared in Dulbecco's modified Eagle medium and added with EGCG 80% (10 μM), EGCG 95% (10 μM), or α-mangosteen (10 μM). After a 24 hours incubation, each passage was measured with the metabolite concentration, SHED-IL10 and SHED-LL37, with human IL-10 and LL37 using enzyme-linked immunosorbent assay. Each different concentration was then analyzed statistically. Results The addition of EGCG 95% is able to stimulate the SHED-IL10 optimum concentration in passage 1 (p < 0.01). But, in the different conditions, the addition of EGCG 80%, EGCG 95%, and α-mangosteen was able to stimulate the SHED-LL37 optimum concentration in passage 2 (p < 0.001). Conclusion The addition of EGCG and α-mangosteen can stimulate the SHED-IL10 and SHED-LL37 concentrations. These two metabolites are promising as regenerative therapy through anti-inflammatory and antibacterial properties.

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Section: Discussionmentioning

confidence: 92%