The ability of supercritical CO<sub>2</sub>carrot and pumpkin extracts to counteract inflammation and oxidative stress in RAW 264.7 macrophages stimulated with LPS or MDA-MB-231 cell-conditioned media

Frattaruolo, Luca; Durante, Miriana; Cappello, Maria Stella; Montefusco, Anna; Mita, Giovanni; Cappello, Anna Rita; Lenucci, Marcello Salvatore

doi:10.1039/d3fo03159d

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…NO is one of main mediators of these processes, playing a central role in the modulation of the release of several inflammatory agents [ 55 , 56 ]. Over the years, several reports have demonstrated that natural products from plants, including red raspberries, are able to combat various disorders, such as inflammation [ 57 , 58 , 59 ].…”

Section: Resultsmentioning

confidence: 99%

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

La Torre,

Loizzo,

Frattaruolo

et al. 2024

Plants

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Raspberry (Rubus idaeus L.) is a fruit of great interest due to its aroma, nutritional properties, and the presence of many bioactive compounds. However, differences among cultivation systems can affect its composition and, consequently, its potential bioactivity. Herein, for the first time, raspberries grown in an aeroponic system were investigated for their chemical profile and antioxidant and anti-inflammatory activity, as well as their enzyme (α-glucosidase and pancreatic lipase) inhibitory properties in comparison to wild and conventionally cultivated fruits. High-performance liquid chromatography coupled with diode array detection (HPLC-DAD) analyses revealed the presence of gallic acid, caffeic acid, chlorogenic acid, p-coumaric acid, ferulic acid, rutin, and catechin in all the samples. The extracts exhibited in vitro anti-inflammatory activity (inhibition of nitric oxide production) regardless of the cultivation method. Of particular interest is the ability of raspberries to inhibit pancreatic lipase. With the exception of the β-carotene bleaching test, the raspberries grown in conventional and aeroponic systems were more active in terms of antioxidants than wild fruits, as evidenced by the ABTS (IC50 in the range 1.6–3.4 μg/mL), DPPH (IC50 in the range 8.9–28.3 μg/mL), and FRAP tests (24.6–44.9 μM Fe(II)/g). The raspberries from aeroponic cultivation were generally able to exert the same bioactivity as those obtained from both conventionally cultivated and wild fruits, supporting the consideration that in the future, this technology could reshape agriculture by mitigating resource constraints, fostering sustainable practices and increasing yields.

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Section: Resultsmentioning

confidence: 99%

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

La Torre,

Loizzo,

Frattaruolo

et al. 2024

Plants

Self Cite

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The SRC/NF‐κB‐AKT/NOS3 axis as a key mediator of Kaempferol's protective effects against oxidative stress‐induced osteoclastogenesis

Shen,

Hu,

Wang

et al. 2024

Immunity Inflam & Disease

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BackgroundOsteoclasts are integral to the advancement of osteoporosis (OP), and their generation under conditions of oxidative stress (OS) involves various pathways. However, the specific mechanism through which the natural antioxidant kaempferol (KAE) mitigates the influence of OS on osteoclasts remains somewhat uncertain. This study aims to evaluate the effect of KAE on osteoclast formation under OS and explore its possible mechanism.MethodsZebrafish were used to observe the effects of KAE on OP and OS. OP and OS "double disease targets" network pharmacology were used to predict the action target and mechanism of KAE on OP under OS. The effects of KAE on osteoclast differentiation induced by OS were evaluated using RWA264.7 cells induced by LPS. To elucidate the potential mechanism, we detected the expression of related factors and target genes during induction.ResultsThe presence of KAE exhibited potential in improving the conditions of OP and OS in zebrafish. KAE can reduce the OS of RAW 264.7 cells stimulated by LPS, inhibit the formation of osteoclasts, and change the level of related factors of OS, and reduce the increase of TRAP. The utilization of network pharmacology and target gene expression assay revealed that KAE exerted a down‐regulatory effect on the expression of proto‐oncogene tyrosine protein kinase (SRC), nuclear factor kappa‐B (NF‐κB), Serine/Threonine Kinase‐1 (AKT1), Nitric Oxide Synthase 3 (NOS3) and Matrix Metallopeptidase‐2 (MMP2).ConclusionBased on the results of this study, KAE may effectively mitigate OS and impede the formation of osteoclasts through the SRC/NF‐κB‐AKT/NOS3 axis.

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Nutritional Qualities and Valorization Trends of Vegetable and Fruit Byproducts: A Comprehensive Review

Tsegay,

Gebreegziabher,

Mulaw

2024

Journal of Food Quality

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Vegetable and fruit byproducts are different parts of vegetables and fruits plus secondary products derived from primary agrofood production processes. With the increasing growth of global vegetable and fruit production, utilization of their byproducts for recovery of health benefit dietary nutrients required emphasis. The current food system and manufacturers are inadequate to maximize the existing food or plant resources’ public demand concurrently to minimize environmental pollution. Important sources of nutritional qualities of these byproducts and their valorization trends are getting research interests. In this review, we have summarized the nutritional qualities and their means of valorization of vegetable and fruit byproducts which have maximum global production from the FAO (2021) published data. The nutritional qualities of the main byproducts are highlighted and the current trends to valorize them into innovative health‐promoting products were assessed. Valorization investigations conducted on these byproducts into food additives (dietary fiber concentrates), nutraceuticals, edible food packaging materials, fermented beverages, and natural dyes were mainly focused on in this review. There are limitations in valorizing technologies of vegetable and fruit byproducts. Hence, further studies are vital to develop novel processing techniques for each of these byproducts. Benefiting from the food waste is a pearl of wisdom and ignoring the value can be participating in pollution.

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The ability of supercritical CO₂carrot and pumpkin extracts to counteract inflammation and oxidative stress in RAW 264.7 macrophages stimulated with LPS or MDA-MB-231 cell-conditioned media

Abstract: This study describes the effects of extraction method (SFE or CSE) and co-solvent composition (hempseed or flaxseed) on carrot and pumpkin extracts ability to counteract inflammation and oxidative stress in LPS-stimulated RAW 264.7 macrophages.

Cited by 4 publications

References 40 publications

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

The SRC/NF‐κB‐AKT/NOS3 axis as a key mediator of Kaempferol's protective effects against oxidative stress‐induced osteoclastogenesis

Nutritional Qualities and Valorization Trends of Vegetable and Fruit Byproducts: A Comprehensive Review

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The ability of supercritical CO2carrot and pumpkin extracts to counteract inflammation and oxidative stress in RAW 264.7 macrophages stimulated with LPS or MDA-MB-231 cell-conditioned media

Abstract: This study describes the effects of extraction method (SFE or CSE) and co-solvent composition (hempseed or flaxseed) on carrot and pumpkin extracts ability to counteract inflammation and oxidative stress in LPS-stimulated RAW 264.7 macrophages.

Cited by 4 publications

References 40 publications

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems

The SRC/NF‐κB‐AKT/NOS3 axis as a key mediator of Kaempferol's protective effects against oxidative stress‐induced osteoclastogenesis

Nutritional Qualities and Valorization Trends of Vegetable and Fruit Byproducts: A Comprehensive Review

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Product

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About

The ability of supercritical CO₂carrot and pumpkin extracts to counteract inflammation and oxidative stress in RAW 264.7 macrophages stimulated with LPS or MDA-MB-231 cell-conditioned media