The Ability of the Peptide Backbone to Bind Anions as well as Cations — Implications for Peptide Carriers, Channels and Electrodes

Eisenman, George; Margalit, Rimona; Kuo, Karen

doi:10.1007/978-3-642-67924-7_1

Cited by 1 publication

(2 citation statements)

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“…Furthermore, after addition of ETH 1097 E,~ approximates E~, and decayed more rapidly with NO;-than with Glu-, again confirming that PNo>PG~. The fact that E m does not quite reach EN, on addition of the ETH 1097 may be due to the low discrimination of this ionophore between Na + and NO~ (PNoJPNa=0.21, Eisenman, Margalit & Kuo, 1981). Figure 3 shows a corresponding experiment for C1-.…”

Section: ]]I-ionic Potential Measurementssupporting

confidence: 66%

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Ion permeability of rabbit intestinal brush border membrane vesicles

1984

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The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS-C3-(5). Tracer measurements provide estimates of the absolute magnitudes of permeability coefficients, while fluorescence measurements provide estimates of relative and absolute ion permeabilities. The magnitudes of the permeability coefficients for Na+, K+, Rb+, and Br- were approximately 5 nanoliters/(mg protein X sec) or 10(-5) cm/sec as determined by radioactive tracer measurements. The apparent selectivity sequence, relative to Na+, as determined by bi-ionic potential measurements was: F-, isethionate, gluconate, choline (less than 0.1) less than Na+(1.0) less than Cl-(1.5) = NO-3(1.5) less than Br-(2.3) less than K+(2.4) less than Rb+(2.5) less than Cs+(2.6) less than Li+(3.9) less than NH+4(12) less than I-(40). The origin of this selectivity sequence and its relationship to the ion permeability of the brush border membrane in the intact epithelium are discussed.

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Section: ]]I-ionic Potential Measurementssupporting

confidence: 66%

“…Since the paracellular pathway is the major route for passive ion fluxes across the the intestine (Frizzell et al, 1973), it is difficult to measure the ion permeabilities of the plasma membranes in the intact tissue. One approach to this problem is to study ion transport in vesicles prepared from brush border and basal-lateral membranes.…”

Section: Introductionmentioning

confidence: 99%