The Ability of Wild Carrot Cell Cultures to Retain Their Capacity for Anthocyanin Synthesis After Storage at -140° C

Abstract

Twenty-five different anthocyanin producing cultures of wild carrot were stored at-140" and recovered. In all CaSes , viable cultures were recovered which produced approximately the Same amount of anthocyanin as did similar cultures which had not been frozen and thawed. This is the first demonstration that plant cell cultures retain their ability to produce specific chemicals after cryogenic Storage. In addition, the data provided show that a reciprocal relationship between growth of cultures and their ability to produce specific chemicals does not always hold true.

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“…The maximum values and the accumulation kinetics were both identical. This is in complete agreement with the results published by Dougall and Whitten (25), who investigated anthocyanin production in different strains of Daucus carota after freezing. Digitalis lanata cells were frozen and thawed as described in Table I.…”

Cryopreservation and Germplasm Storage

“…A number of examples are now available to depict that the cryopreserved cells maintain their embryogenic potential or plant regeneration capability (for a review see Withers, 1985). For example, 25 different anthocyanin producing cell cultures of wild carrot produced approximately the same amount of anthocyanin after cryogenic storage as did the unfrozen controls (Dougall and Whitten, 1980). For example, 25 different anthocyanin producing cell cultures of wild carrot produced approximately the same amount of anthocyanin after cryogenic storage as did the unfrozen controls (Dougall and Whitten, 1980).…”

Cryopreservation of Alkaloid-Producing Cell Cultures of Periwinkle (Catharanthus roseus)

“…Storage in LN2 followed by recovery of viable plant cells have been reported for many species (7,10,12,14,21,22,28,30) and cells of certain species retained the biosynthetic capacity for biotin (25), anthocyanin (8), and cardenolides (7). However, whether such a cryogenic technique could be applied to the preservation of cell cultures with capability for alkaloid synthesis and accumulation has not been determined and thus served as the primary objective of the present study.…”

“…The production of alkaloids by in vitro cultures can vary among cell lines and in individual lines be fairly stable (2, 4); in other cell lines, alkaloid content and spectra have been found subject to gradual change over years of subculture (6). Instead of repeated selection to maintain a high alkaloid level, cryopreservation would appear as an alternative to overcome stability problems (14, 31).Storage in LN2 followed by recovery of viable plant cells have been reported for many species (7,10,12,14,21,22,28,30) and cells of certain species retained the biosynthetic capacity for biotin (25), anthocyanin (8), and cardenolides (7). However, whether such a cryogenic technique could be applied to the preservation of cell cultures with capability for alkaloid synthesis and accumulation has not been determined and thus served as the primary objective of the present study.…”