The ABO blood grouping of a minute hair sample by the immunohistochemical technique

Miyasaka, Sachio; Yoshino, Mineo; Sato, Hajime; Miyake, B; Seta, Sueshige

doi:10.1016/0379-0738(87)90087-9

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…Recently, indirect immunoperoxidase methods have been used to locate ABH blood group antigens in tissue sections and in 1984 the Japanese groups of Miyasaka et al and Yoshida and Ono investigated this method and suggested its possible application for the detection of ABH antigens in hair. These investigations were followed by several reports which confirmed the usefulness of this method (P6tsch-Schneider et al 1986;Miyasaka et al 1987). The present investigation was carried out to test the reliability and reproducibility of ABH antigen detection in hairs obtained from Europeans using the Avidin-Biotin Complex (ABC).…”

Section: Introductionsupporting

confidence: 63%

ABO blood grouping of hairs using an Avidin-Biotin-Peroxidase Complex technique

et al. 1989

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Summary. Fifty-nine hair specimens obtained from human autopsies and volunteers were used for the determination of ABO blood group substances using the ABC (Avidin-Biotin Complex) technique. Positive staining for A, B and H blood group substances was detected only in the medulla of the hairs. Blood group antigens could not be detected in seven hair specimens because they possessed no medulla. Forty-seven specimens obtained from fresh cadavers and volunteers gave the correct results corresponding to the blood group of the donor, but some specimens from individuals of blood group A2, L e ( a + b -) showed weak reaction with anti-A and strong reaction with anti-H. The staining intensity with anti-B and -H in some individuals of blood group AB was stronger than with anti-A serum. Five hair specimens obtained from decomposed bodies were also examined. The blood group antigens could be specifically detected in hairs obtained from two exhumed and one putrid body, but no positive reactions were obtained from two cases of drowning where the bodies had been in the sea for about 6 months. In a blind trial, hair specimens from 28 individuals were also examined. Twentytwo specimens which possessed a medulla gave the correct result. Six specimens gave no result because they possessed no medulla. Key words: ABO grouping on hairs, immunohistochemistry -Avidin-BiotinComplex, ABO grouping on hairsZusammenfassung. An 59 menschlichen Haarproben von Leichen und lebenden Personen wurden die ABO-Blutgruppen mittels der Avidin-BiotinTechnik untersucht.Eine positive Reaktion for A-, B-und H-Blutgruppen-Antigene konnte nur im Markstrang beobachtet werden. Wegen eines fehlenden Markstranges gelang der Nachweis in einigen Proben nicht.Bei 47 Haarproben von frischen Leichen und Lebenden stimmte das Untersuchungsergebnis mit der festgestellten Blutgruppe ~iberein, aber einige Proben von Personen der Blutgruppe A2, L e ( a + b -) zeigten eine schwache Reaktion mit Anti-A und eine starke mit Anti-H. Die Reaktion mit Anti-B

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Section: Introductionsupporting

confidence: 63%

ABO blood grouping of hairs using an Avidin-Biotin-Peroxidase Complex technique

et al. 1989

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“…Various blood grouping methods, including that of the ABO blood type, were used for person identification when DNA typing was not available. However, ABO blood grouping is now often omitted, and it sometimes provides valuable information for narrowing down the subjects for DNA typing because the ABO blood group can be determined, not only from bloodstains, but also from saliva, semen, and hair [24][25][26]. As mentioned above, DNA typing is based on the STR type and sex determination on the amelogenin gene, and there is no doubt about their personal identification power.…”

Section: Flow Of Forensic Examinations Using Biological Samplesmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Current research and case work activities of criminalistics in Japan

Seta

1996

Forensic Science International

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The ABO blood grouping of a minute hair sample by the immunohistochemical technique

Cited by 7 publications

References 11 publications

ABO blood grouping of hairs using an Avidin-Biotin-Peroxidase Complex technique

ABO blood grouping of hairs using an Avidin-Biotin-Peroxidase Complex technique

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current research and case work activities of criminalistics in Japan

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