The absence of an association between Interleukin 1β gene polymorphisms and recurrent aphthous stomatitis (RAS)

Ślebioda, Zuzanna; Kowalska, Anna; Rozmiarek, Marta; Krawiecka, Ewa; Szponar, Elżbieta; Dorocka‐Bobkowska, Barbara

doi:10.1016/j.archoralbio.2017.09.013

Cited by 10 publications

(9 citation statements)

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“…1,2 The etiopathogenesis of RAS remains unclear, but an abnormal immune interaction within the oral mucosal cells caused by the impaired activation of the immune system in genetically predisposed subjects has been considered to be an essential contributor in this process. [3][4][5] In aphthous lesions, the barrier function of the oral epithelium is destroyed. The exposure of danger-signal receptors, such as Toll-like receptors (TLRs), to pathogen-or microbe-associated (PAMP/MAMP) and damage-associated (DAMP) molecular patterns leads to an acute inflammatory reaction with cytokine production in the epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…Systemic diseases and medications have also been suggested as the potential triggers of RAS in several studies, although the results remain equivocal. 5,7,8 Familial accumulation was observed in up to 40% of patients with RAS. 3,9 The genetic risk factors determining an individual's susceptibility to the disease include various DNA polymorphisms distributed in the human genome, especially those related to alterations in the metabolism of cytokines.…”

Section: Introductionmentioning

confidence: 99%

“…3,9 The genetic risk factors determining an individual's susceptibility to the disease include various DNA polymorphisms distributed in the human genome, especially those related to alterations in the metabolism of cytokines. 5,10 Recent research has suggested that dysregulation in the production of defensins might be involved in the development of several oral pathologies, including those with an autoimmune background. 6,11 Aphthous lesions may be associated with systemic diseases, such as Behçet's disease, Crohn's disease, HIV infection, anemia, cyclic neutropenia, and celiac disease.…”

Section: Introductionmentioning

confidence: 99%

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Low-level laser therapy (LLLT) in the treatment of recurrent aphthous stomatitis (RAS) – a promising treatment option: A report of two cases

2019

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Recurrent aphthous stomatitis (RAS) is a chronic, ulcerative condition of the oral mucosa that affects 10-25% of the population. The etiopathogenesis of the disease is not fully understood, although a polygenic mode of inheritance and immunological dysregulation have been suggested in several studies. The contribution of numerous predisposing factors, such as a deficiency of iron, vitamin B12 or folic acid, trauma, emotional stress, endocrine disturbances, or allergy, have also been considered. So far, no causative treatment for RAS has been developed; instead, topical and systemic drugs are used to reduce pain and inflammation, and to lengthen the period of remission. Low-level laser therapy (LLLT) is a non-invasive and atraumatic therapeutic method that involves the local application of a high-density, monochromatic, narrow-band light source. With the use of the appropriate power and wavelength, the therapy brings anti-inflammatory and analgesic results, and wound healing is promoted. Several reports on the beneficial effects of LLLT in RAS have been presented recently. This report describes 2 cases of adult patients with RAS treated with LLLT to relieve pain and promote the healing of the ulcers. The clinical presentations with the signs and symptoms are discussed and illustrated, along with the treatment algorithms and outcomes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low-level laser therapy (LLLT) in the treatment of recurrent aphthous stomatitis (RAS) – a promising treatment option: A report of two cases

2019

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“…There are many methods for SNP genotyping, including MALDI‐TOF mass spectrometry, Taqman probe, restriction fragment length polymorphism (RFLP), microarray, and DNA sequencing . Due to the high cost, MALDI‐TOF mass spectrometry, microarray, and DNA sequencing are not suitable for analysis of large samples.…”

Section: Introductionmentioning

confidence: 99%

Characteristic and influencing factors of Taqman genotyping calling error

Xing

Liu

et al. 2018

Clinical Laboratory Analysis

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Background Taqman fluorescent probe was frequently applied in single nucleotide polymorphism (SNP) genotyping. However, the characteristic of calling error and the influencing factors remain unclear. Method Calling errors of Taqman genotyping was evaluated systematically based on Mendelian inheritance. Twenty‐two SNPs were genotyped by Taqman probe for 419 pedigrees. Mendelian genetic errors were counted for every SNP and pedigree. Cluster analysis was applied to investigate the compatibility between Taqman probes and DNA sample. Results On one hand, errors were found for all the SNPs. The error number ranged from 4 to 33 with median of 10.5. On the other hand, Mendelian genetic errors showed features of both randomness and cluster. Half of the pedigrees containing errors had only 1 Mendelian genetic error. But there was also a pedigree containing up to 10 Mendelian genetic errors. Furthermore, cluster analysis indicated that errors of different SNPs took place in different pedigree cluster. Conclusion It could be concluded that calling error is inevitable for Taqman genotyping of large samples. The quality of Taqman probe and DNA sample, as well as their compatibility, may account for the error incidence.

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“…Positive associations were found between RAS and selected IL1 , IL2 , IL4 , IL6, and IL10 gene polymorphisms. Interestingly, only 4 genetic studies have focused on the gene variability of interleukins in European Caucasian populations with RAS , even though the prevalence of this disease is comparable across populations . As IL1 and IL6 gene polymorphisms have already been studied in RAS patients from the Czech Republic , the current study deals with IL2 , IL4 and its receptor α, IL4Rα , IL10 , and IL13 .…”

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Recurrent aphthous stomatitis and gene variability in selected interleukins: a case–control study

Linhartová

Jánoš

Slezaková

et al. 2018

European J Oral Sciences

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Genetic factors, especially those related to immune system functioning, have been intensively studied to determine their role in the development of recurrent aphthous stomatitis (RAS). The aim of the present study was to analyze gene variability in interleukin (IL)2, IL4 (and its receptor α, IL4Rα), IL10, and IL13, which were selected based on literature review and/or their functional relevance, in Czech patients with RAS and in healthy controls. In total, 252 subjects (178 controls and 74 patients with RAS) were enrolled in this case–control study, and their detailed anamnestic, clinical, and laboratory data were obtained. Nine polymorphisms in the genes encoding interleukins were determined using PCR techniques. There were no significant differences in allele or genotype frequencies of the IL2, IL4, IL4Rα, IL10, and IL13 polymorphisms rs2069762/rs2069763, rs2243250/rs79071878, rs1801275, rs1800896, and rs1800925, respectively, between controls and patients with RAS. The minority alleles rs1800871 and rs1800872, which encode variants of IL10, were associated with a statistically significantly higher risk of RAS, as confirmed by the results of genotype and haplotype analyses. We suggest that variability in the IL10 gene may play an important role in the development of RAS in the Czech population.

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The absence of an association between Interleukin 1β gene polymorphisms and recurrent aphthous stomatitis (RAS)

Cited by 10 publications

References 32 publications

Low-level laser therapy (LLLT) in the treatment of recurrent aphthous stomatitis (RAS) – a promising treatment option: A report of two cases

Low-level laser therapy (LLLT) in the treatment of recurrent aphthous stomatitis (RAS) – a promising treatment option: A report of two cases

Characteristic and influencing factors of Taqman genotyping calling error

Recurrent aphthous stomatitis and gene variability in selected interleukins: a case–control study

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