The absorption of phospholipid vesicles by perfused rat liver depends on vesicle surface charge

Nicholas, A.R.; Jones, Malcolm N.

doi:10.1016/0005-2736(86)90559-6

Cited by 12 publications

(2 citation statements)

References 23 publications

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“…Finally, equal amounts of liposome-encapsu lated and free drug can have different efficacies owing to their localization to particular subcellular compartments (Nicholas and Jones, 1986).…”

Section: Discussionmentioning

confidence: 99%

Liposomal Encapsulation of Ganciclovir Enhances the Efficacy of Herpes Simplex Virus Type 1 Thymidine Kinase Suicide Gene Therapy against Hepatic Tumors in Rats

Engelmann

Bolard²,

Diquet³

et al. 1999

Human Gene Therapy

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Suicide gene therapy based on ganciclovir (GCV) metabolism by transgene herpes simplex thymidine kinase (HSV-1 TK) has been used to selectively kill proliferating cells in clinical settings such as cancer, vascular restenosis, and immunological disorders. We investigated whether encapsulation of ganciclovir (GCV) into liposomes would improve its efficacy, especially against hepatic tumors. Large unilamellar liposomes containing GCV were prepared by reversed-phase evaporation. Pharmacokinetic studies in rats showed that, compared with free GCV, the intravenous injection of liposome-encapsulated GCV (lip-GCV) led to a faster decrease in GCV plasma concentrations, but higher liver-blood ratios. After treatment of syngeneic HSV-1 TK+ liver metastases in rats, histologically active tumors were found in 95% of the transplanted lesions when physiological saline had been given and in 50% when free GCV had been given at 90.2 microM/kg twice daily. This dose is known to be insufficient for the eradication of HSV-1 TK+ tumors. In contrast, only 5% viable tumors were found in rats receiving lip-GCV at this same concentration. Average tumor volumes were 19 +/- 15, 7 +/- 9, and <1 mm3 for the control, free GCV, and lip-GCV groups, respectively. GCV-related toxicity was no longer observed. The results demonstrate that liposomal encapsulation of GCV is feasible and significantly enhances its efficacy against HSV-1 TK+ hepatic tumors.

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“…Finally, equal amounts of liposome-encapsu lated and free drug can have different efficacies owing to their localization to particular subcellular compartments (Nicholas and Jones, 1986).…”

Section: Discussionmentioning

confidence: 99%

Liposomal Encapsulation of Ganciclovir Enhances the Efficacy of Herpes Simplex Virus Type 1 Thymidine Kinase Suicide Gene Therapy against Hepatic Tumors in Rats

Engelmann

Bolard²,

Diquet³

et al. 1999

Human Gene Therapy

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“…The ZP of FeO adducts with lipophilic TAs such as FuGene6 and Lipofectamine was considerably smaller (ZP ≈+10 mV) or, as in the case of Lipofectamine, at concentrations less than 2 μg/mL even slightly negative (Figure 1C). Two features of the FeO particles seem to be relevant for high uptake of FeO/TA complexes: a particle size of the order of 100 μm (and potentially more) and reasonably high positive charge [32–34]. The latter is in line with the observation that membrane-penetrating peptides comprise a large number of positively charged amino acids such as arginine [35].…”

Section: Discussionmentioning

confidence: 83%

Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents

et al. 2006

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Visualization and quantification of inflammatory processes is of high importance for early diagnosis of a multitude of diseases. Magnetic resonance imaging (MRI) using iron oxide (FeO) nanoparticles as contrast agents allows the study of macrophage infiltration during inflammation in a variety of tissues. Macrophages are effectors of the immune response, their appearance being orchestrated by activated T lymphocytes. Therefore, tracking of labeled T lymphocytes, which initiate the immune process, should enable earlier detection of tissue inflammation. In this study, we investigate the feasibility of specifically labeling harvested T cells by using dextran-coated FeO nanoparticles and commonly available transfection agents (TAs). Physicochemical properties of the newly formed FeO/TA vesicles were determined as well as their cell toxicity and their T cell activation potential. The labeling efficiency of each FeO/TA combination was evaluated by measuring the transverse MRI relaxation rate R(2) by X-ray spectroscopy and magnetic selection. Toxicity and labeling efficacy differed significantly among TAs. The best results were achieved by using polyamine TAs and in particular by using poly-l-lysine at a concentration of 1.5 microg/mL administered in combination with 22.5 microg iron/mL. By using this protocol, up to 60% of harvested T cells could be labeled. Microscopic investigation revealed FeO/TA nanoparticles not only localized within the cytoplasma of the cells but also sticking to the outer membrane surface.

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Isothermal volume variations in lipid vesicle suspensions. A new evidence of intervesicle fusion kinetics

Zuccarello

Raudino

et al. 1991

Il Nuovo Cimento D

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The absorption of phospholipid vesicles by perfused rat liver depends on vesicle surface charge

Cited by 12 publications

References 23 publications

Liposomal Encapsulation of Ganciclovir Enhances the Efficacy of Herpes Simplex Virus Type 1 Thymidine Kinase Suicide Gene Therapy against Hepatic Tumors in Rats

Liposomal Encapsulation of Ganciclovir Enhances the Efficacy of Herpes Simplex Virus Type 1 Thymidine Kinase Suicide Gene Therapy against Hepatic Tumors in Rats

Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents

Isothermal volume variations in lipid vesicle suspensions. A new evidence of intervesicle fusion kinetics

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