The abundance and diversity of gut bacteria of rice leaffolder Cnaphalocrocis medinalis (Guenée) across life stages

Yang, Yajun; Xiaogai, Liu; Xu, Hongxing; Liu, Ying‐Hong; Ali, M. P.; Bodlah, Muhammad Adnan; Lü, Zhongxian

doi:10.1016/j.aspen.2020.03.006

Cited by 17 publications

(21 citation statements)

References 34 publications

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“…Considering the limited gut bacterial information isolated and cultured by traditional methods ( Yang, 2012 ), we obtained the bacterial information of C. medinalis by MiSeq sequencing. Recently, we reported that the composition of the gut bacterial community changes across the life cycle of C. medinalis , and the phyla Proteobacteria and Firmicutes were the dominant bacterial taxa ( Yang et al, 2020a ). In the guts of both C. medinalis fed rice and maize, the phyla Proteobacteria and Firmicutes were also the dominant bacterial taxa.…”

Section: Discussionmentioning

confidence: 99%

“…In lepidopterans, metamorphosis, which entails major morphological changes with dietary transformation, could also have a strong impact on the gut microbiota composition ( Voirol et al, 2018 ). However, certain taxa can persist throughout all the stages of the insect ( Hammer et al, 2014 ; Yang et al, 2020a ). In insects, the gut microbiota can promote gut homeostasis ( Buchon et al, 2013 ), and core microbes in the gut microbiota may reach homeostasis by interacting with the factors in the environment.…”

Section: Discussionmentioning

confidence: 99%

“…By comparison, a large number of gut microbiota were obtained from C. medinalis larvae through Illumina MiSeq technology ( Liu et al, 2016 ). Yang et al (2020a) analyzed the gut microbiota composition of C. medinalis across the developmental stages. Information on the host-associated changes in gut bacteria will facilitate the overall understanding of insect ecology and promote the development of novel methods for pest management.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Host Plant and Insect Generation on Shaping of the Gut Microbiota in the Rice Leaffolder, Cnaphalocrocis medinalis

Yang

Xiaogai

et al. 2022

Front. Microbiol.

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Gut microbes in insects may play an important role in the digestion, immunity and protection, detoxification of toxins, development, and reproduction. The rice leaffolder Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is a notorious insect pest that can damage rice, maize, and other gramineous plants. To determine the effects of host plants and generations on the gut microbiota of C. medinalis, we deciphered the bacterial configuration of this insect pest fed rice or maize for three generations by Illumina MiSeq technology. A total of 16 bacterial phyla, 34 classes, 50 orders, 101 families, 158 genera, and 44 species were identified in C. medinalis fed rice or maize for three generations. Host plants, insect generation, and their interaction did not influence the alpha diversity indices of the gut microbiota of C. medinalis. The dominant bacterial taxa were Proteobacteria and Firmicutes at the phylum level and Enterococcus and unclassified Enterobacteriaceae at the genus level. A number of twenty genera coexisted in the guts of C. medinalis fed rice or maize for three generations, and their relative abundances occupied more than 90% of the gut microbiota of C. medinalis. A number of two genera were stably found in the gut of rice-feeding C. medinalis but unstably found in the gut microbiota of maize-feeding C. medinalis, and seven genera were stably found in the gut of maize-feeding C. medinalis but unstably found in the gut of rice-feeding C. medinalis. In addition, many kinds of microbes were found in some but not all samples of the gut of C. medinalis fed on a particular host plant. PerMANOVA indicated that the gut bacteria of C. medinalis could be significantly affected by the host plant and host plant × generation. We identified 47 taxa as the biomarkers for the gut microbiota of C. medinalis fed different host plants by LEfSe. Functional prediction suggested that the most dominant role of the gut microbiota in C. medinalis is metabolism, followed by environmental information processing, cellular processes, and genetic information processing. Our findings will enrich the understanding of gut bacteria in C. medinalis and reveal the differences in gut microbiota in C. medinalis fed on different host plants for three generations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Host Plant and Insect Generation on Shaping of the Gut Microbiota in the Rice Leaffolder, Cnaphalocrocis medinalis

Yang

Xiaogai

et al. 2022

Front. Microbiol.

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“…vigintioctopunctata fourth instar larvae, the predominant microorganisms were unclassified Enterobacteriaceae (Enterobacteriaceae), whereas in this study, Acinetobacter (Moraxellaceae) was predominant in both groups. These differences may reflect differences in geographic populations ( Wang et al 2020 ), life stages ( Yang et al 2020 ), growth environments, or nutrient accumulation in the host plant. Studies have shown that although host plants are the primary driving factors of gut microbial composition ( Jones et al 2019 ), other factors, including habitat ( Paniagua et al 2018 ) and developmental stage ( Yun et al 2014 ), can also affect gut microbial composition in insects.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Gut Microbiota on the Fecundity of Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

Zhao

Yang

et al. 2021

Journal of Insect Science

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The gut microbiota of insects usually plays an important role in the development and reproduction of their hosts. The fecundity of Henosepilachna vigintioctopunctata (Fabricius) varies greatly when they develop on different host plants. Whether and how the gut microbiota regulates the fecundity of H. vigintioctopunctata was unknown. To address this question, we used 16S rRNA sequencing to analyze the gut microbiomes of H . vigintioctopunctata adults fed on two host plant species ( Solanum nigrum and Solanum melongena ) and one artificial diet. The development of the ovaries and testes was also examined. Our results revealed that the diversity and abundance of gut microorganisms varied significantly in insects reared on different diets. The gut microbiota of H . vigintioctopunctata raised on the two host plants was similar, with Proteobacteria being the dominant phylum in both groups, whereas Firmicutes was the dominant phylum in the group reared on the artificial diet. The predominant microbiota in the S . nigrum group were Acinetobacter soli and Acinetobacter ursingii ( Acinetobacter , Moraxellaceae); Moraxella osloensis ( Enhydrobacter , Moraxellaceae); and Empedobacter brevis ( Empedobacter , Weeksellaceae). The microbiota in this group are associated with high lipid metabolism. In addition, the beetles’ ovaries and testes were more highly developed in the S . nigrum group than in the other two groups. These findings provide valuable information for elucidating the complex roles the gut microbiota play in the fecundity of H . vigintioctopunctata , and may also contribute to developing future novel control strategies involving this economically important pest.

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“…All of the indices used for our samples were calculated with QIIME (Version 1.7.0). The Mothur method and the SSUrRNA database [23] of SILVA132 (http://www.arb-silva.de/ (accessed on 25 March 2019)) [24] were used to perform species annotation analysis (with threshold values of 0.8~1) to obtain taxonomic information and classification level counts in order to determine the community composition of each sample. This was conducted to show the diversity of the gut microbiota at the phylum and genus levels in L. xylina fed on different diets, using GraphPad Prism software (Version 6.01).…”

Section: Gut Microbiota Composition Of L Xylina On Different Dietsmentioning

confidence: 99%

Gut Bacterial Communities of Lymantria xylina and Their Associations with Host Development and Diet

Cui

Chu

et al. 2021

Microorganisms

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The gut microbiota of insects has a wide range of effects on host nutrition, physiology, and behavior. The structure of gut microbiota may also be shaped by their environment, causing them to adjust to their hosts; thus, the objective of this study was to examine variations in the morphological traits and gut microbiota of Lymantria xylina in response to natural and artificial diets using high-throughput sequencing. Regarding morphology, the head widths for larvae fed on a sterilized artificial diet were smaller than for larvae fed on a non-sterilized host-plant diet in the early instars. The gut microbiota diversity of L. xylina fed on different diets varied significantly, but did not change during different development periods. This seemed to indicate that vertical inheritance occurred in L. xylina mutualistic symbionts. Acinetobacter and Enterococcus were dominant in/on eggs. In the first instar larvae, Acinetobacter accounted for 33.52% of the sterilized artificial diet treatment, while Enterococcus (67.88%) was the predominant bacteria for the non-sterilized host-plant diet treatment. Gut microbe structures were adapted to both diets through vertical inheritance and self-regulation. This study clarified the impacts of microbial symbiosis on L. xylina and might provide new possibilities for improving the control of these bacteria.

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The abundance and diversity of gut bacteria of rice leaffolder Cnaphalocrocis medinalis (Guenée) across life stages

Cited by 17 publications

References 34 publications

Effects of Host Plant and Insect Generation on Shaping of the Gut Microbiota in the Rice Leaffolder, Cnaphalocrocis medinalis

Effects of Host Plant and Insect Generation on Shaping of the Gut Microbiota in the Rice Leaffolder, Cnaphalocrocis medinalis

The Influence of Gut Microbiota on the Fecundity of Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

Gut Bacterial Communities of Lymantria xylina and Their Associations with Host Development and Diet

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