The accumulation and histological effects of organometallic fungicides Propineb and Maneb in the kidneys of fetus and female rats during pregnancy

Güven, Kemal; Deveci, Engin; Akba, Osman; Önen, Abdurrahman; Pomerai, David de

doi:10.1016/s0378-4274(98)00128-3

Cited by 26 publications

(14 citation statements)

References 29 publications

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“…Female rats treated with 250 ppm maneb during pregnancy show elevated renal concentrations of Mn as compared with controls, but this difference is far more marked in the newborn offspring, whose body weights are also significantly reduced in the maneb-treated group (see Reference 7). Similar findings are also reported [7] for the Zn-containing dithiocarbamate propineb, suggesting efficient transplacental accumulation of these organometallic fungicides. This is supported by the lipophilic nature of dithiocarbamates containing chelated metals; exchange reactions in solution may allow these agents to carry other toxic metal ions (e.g., Cu, Pb) across plasma membranes, thereby facilitating their entry into living organisms [8].…”

Section: Introductionsupporting

confidence: 85%

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain ofCaenorhabditis elegans

Güven

Power

Avramides

et al. 1999

J. Biochem. Mol. Toxicol.

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Section: Introductionsupporting

confidence: 85%

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain ofCaenorhabditis elegans

Güven

Power

Avramides

et al. 1999

J. Biochem. Mol. Toxicol.

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“…In oral studies in rats it was shown that Zn 2+ concentration is dose dependently increased in liver and kidney tissue (Deveci et al 1999;Guven et al 1998). In these studies, normal zinc accumulation/wet weight was around 150 ppm in the kidney and 75 ppm in the liver.…”

Section: Discussionmentioning

confidence: 90%

Effects of the dithiocarbamate fungicide propineb in primary neuronal cell cultures and skeletal muscle cells of the rat

Schmuck

Ahr

Mihail

et al. 2002

Archives of Toxicology

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After repeated-dose toxicity studies with the fungicide propineb, reversible effects on muscle functions were found. Therefore, mechanistic investigations should contribute to clarification of its mode of action in relation to disulfiram and diethyldithiocarbamate neurotoxicity or direct effects on muscle cells. In principle, besides the dithiocarbamate effects, two different mechanisms have been discussed for this fungicide. One mechanism is the degradation to carbon disulfide (CS(2)) and propylenthiourea (PTU) and the other are direct effects of zinc. Primary neuronal cell cultures of the rat are a well established model to identify neurotoxic compounds like n-hexane or acrylamide. In this cell culture model, endpoints such as viability, energy supply, glucose consumption and cytoskeleton elements were determined. Additionally, skeletal muscle cells were used for comparison. Propineb and its metabolite PTU were investigated in comparison to CS(2), disulfiram and diethyldithiocarbamate. The toxicity of zinc was tested using zinc chloride (ZnCl(2)). It was clearly shown that propineb exerted strong effects on the cytoskeleton of neuronal and non-neuronal cell cultures (astrocytes, muscle cells). This was similar to ZnCl(2,) but not to CS(2). With CS(2) and disulfiram effects on the energy supply were more prominent. In conclusion, the toxicity of propineb is not comparable to disulfiram, diethyldithiocarbamate or CS(2) neurotoxicity. In regard to these findings, a direct reversible effect of propineb on skeletal muscle cells seems to be more likely.

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“…Female rats treated with maneb at 250 ppm during pregnancy showed elevated renal concentrations of Mn as compared with controls. This difference was more marked in newborn offspring due to transplacental accumulation (Guven et al, 1998).…”

Section: Discussionmentioning

confidence: 92%

“…Although classed as of low to intermediate toxicity, the dithiocarbamates raise some toxicological concerns because they can be metabolized to release free Mn (and/or Zn) and ethylene thiourea (ETU), a possible vertebrate carcinogen (Houeto et al, 1995;Vettorazzi et al, 1995). Their use has increasingly been associated with genotoxic, teratogenic, and cytotoxic effects (Espigares et al, 1998;Vettorazzi et al, 1995;Guven et al, 1998Guven et al, , 1999. It has also been shown that they have demonstrable adverse effects on several nontarget organisms, including nematodes (Guven et al, 1999;Easton et al, 2001), protozoans (Bonnemain and Dive, 1990), and some soil microbial populations (Pozo et al, 1994), as well as on enzyme biosynthesis in E. coli and Bacillus subtilis (Guven et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The effects of organic pesticides on inner membrane permeability in Escherichia coli ML35

et al. 2005

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We have tested whether some pesticides might cause inner membrane leakage in ML35 Escherichia coli cells, which express beta-galactosidase (lacZ; EC 3.2.1.23) constitutively but lack the permease (lacY) required for substrate entry. The activity of beta-galactosidase (indicative of substrate leakage through the inner membrane) was increased by various concentrations of pesticides, including the organometallic fungicides maneb and mancozeb, the insecticide Thiodan, and the herbicide Ally, as well as by antibiotics such as ampicillin, gramicidin D, and the calcium ionophore A23187. The enzyme activity was increased by up to approximately 30% when the E. coli ML35 strain was exposed to various concentrations (between 50 and 250 ppm) of both fungicides. Thiodan had only a slight effect on beta-galactosidase activity (increase of 12.8%), whereas, among the antibiotics, the calcium ionophore at 20 microg/ml caused a significant increase in enzyme activity by up to 61.8%. This effect is similar to that of sodium dodecyl sulfate, used as positive control ( approximately 70% increase). Accumulation of maneb and mancozeb by bacterial cells was also studied taking advantage of their metal content and using atomic absorption spectrophotometry. In parallel with the increase in enzyme activity, both fungicides accumulated in the cells as a function of their concentration. Time course experiments (3, 6, and 9 h) of fungicide accumulation and of bacterial growth at various pesticide concentrations were also carried out. Maneb seems to inhibit the bacterial growth better than mancozeb. In addition, maneb uptake increases with time up to 9 h at all tested concentrations, whereas the accumulation of mancozeb is similar at all the exposure times tested. This indicates a different uptake and/or metabolizing strategy by E. coli cells for the two fungicides.

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The accumulation and histological effects of organometallic fungicides Propineb and Maneb in the kidneys of fetus and female rats during pregnancy

Cited by 26 publications

References 29 publications

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain ofCaenorhabditis elegans

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain ofCaenorhabditis elegans

Effects of the dithiocarbamate fungicide propineb in primary neuronal cell cultures and skeletal muscle cells of the rat

The effects of organic pesticides on inner membrane permeability in Escherichia coli ML35

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