The Accumulation of Divalent Ions by Isolated Mitochondria

Chappell, Joseph; Cohn, Mildred; Greville, G. D.

doi:10.1016/b978-1-4832-2840-2.50029-x

Cited by 80 publications

(43 citation statements)

References 9 publications

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“…Finally, it has been found that isolated mitochondria accumulate Mg ++ (12)(13)(14) in a respiration-linked process. The accumulation of Mn ++ (7), Ba++ (7,11) and Sr ++ (7,11) has also been briefly reported.…”

Section: Introductionmentioning

confidence: 98%

“…The "active" or respiration-linked accumulation of Ca ++ from the suspending medium by isolated mitochondria, first demonstrated by Vasington and Murphy (1,2), has been studied in some detail in a number of recent investigations (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). The accumulation of Ca ++ during respiration has been found to be quite massive, attaining values as high as 3.0/~moles Ca++ per mg mitochondrial protein (2)(3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Greenawalt

Rossi

Lehninger

1964

The Journal of Cell Biology

357

126

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Rat liver mitochondria allowed to accumulate maximal amounts of Ca ++ and HPO4 = ions from the suspending medium in vitro during respiration have a considerably higher specific gravity than normal mitochondria and may be easily separated from the latter by isopycnic centrifugation in density gradients of sucrose or cesium chloride. When the mitochondria are allowed to accumulate less than maximal amounts of Ca++ and HPO4= from the medium, they have intermediate specific gravities which are roughly proportional to their content of calcium phosphate. Maximally "loaded" mitochondria are relatively homogeneous with respect to specific gravity. Correlated biochemical and electron microscopic studies show that Ca++-loaded mitochondria contain numerous dense granules, of which some 85 per cent are over 500 A in diameter. These granules are electron-opaque not only following fixation and staining with heavy metal reagents, but also following fixation with formaldehyde, demonstrating that the characteristic granules in Ca++-loaded mitochondria have intrinsic electron-opacity. The dense granules are almost always located within the inner compartment of the mitochondria and not in the space between the inner and outer membranes. They are frequently located at or near the cristae and they often show electrontransparent "cores." Such granules appear to be made up of clusters of smaller dense particles, but preliminary x-ray diffraction analysis and electron diffraction studies have revealed no evidence of crystallinity in the deposits. The electron-opaque granules decrease in number when the Ca++-loaded mitochondria are incubated with 2,4-dinitrophenol; simultaneously there is discharge of Ca ++ and phosphate from the mitochondria into the medium.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Greenawalt

Rossi

Lehninger

1964

The Journal of Cell Biology

357

126

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“…Consequently, it appears to be unrelated to the mitochondrial contraction which requires energy THE ffOUENAL OF CELL BIOLOGY • VOLVME 56, 1973 • pages 51-64 31 from either ATP hydrolysis (5)(6)(7)(8)(9)(10)(11) or respiration (9)(10)(11)(12) and which is associated with decrease of inner compartment volume and condensation of the matrix (11)(12)(13)(14). On the other hand, it is evident from similarities in nucleotide specificity, inhibitor sensitivity, and ultrastructural changes that the inner membrane contraction phenomenon is identical with the adenine nucleotide-induced transformation of heart mitochondria into highly condensed morphological states described by Weber and Blair (15,16).…”

Section: Introductionmentioning

confidence: 99%

Adenine Nucleotide-Induced Contraction of the Inner Mitochondrial Membrane

Stoner

Sirak

1973

The Journal of Cell Biology

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The inner membranes of isolated bovine heart mitochondria undergo pronounced contraction upon being exposed to exogenous adenosine diphosphate (ADP), adenosine triphosphate (ATP), and certain other hlgh-energy phosphate compounds. Contraction results in decrease of inner membrane expanse which in turn results in decrease of intracristal space and increase of mitochondrial optical density (OD). The magnitude of the OD change appears to be proportional to the degree of contraction Half-maximal contraction can be achieved with ADP or ATP at concentrations as low as about 0 3 /zM. Atractyloside at concentrations as low as about 1.2 nmol/mg mitochondrial protein completely inhibits the contraction. It is concluded from these and other observations that inner membrane contraction occurs as a result of adenine nucleotide binding to the carrier involved in the exchange of adenine nucleotides across the inner mitochondrial membrane.

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“…At least one of the reactions is reversible and reducing power can be provided at the expense of ATP. Indirect support for a reaction of this kind in active transport is found in work with mitochondria, which make use of a high-energy intermediate both for amino acid incorporation (Bronk, 1963) and potassium uptake (Brierley, 1963;Chappell, Cohn & Greville, 1963). Glynn (1963Glynn ( , 1966 has discussed the hypothesis and pointed out that active transport and oxidative phosphorylation might share a common step, although the processes occur in different cell structures.…”

Section: Deoxyadenosine and Potassium Influxmentioning

confidence: 99%

Potassium transport and nucleoside metabolism in human red cells

Whittam¹,

Wiley²

1967

The Journal of Physiology

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SUMMARY1. A study has been made of active cation transport in relation to the metabolism of nucleosides and deoxynucleosides in human red blood cells.2. Lactate production from inosine depended on the cellular inorganic phosphate available for phosphorolysis and was raised to some 4 times the normal rate from glucose. Inosine inhibited glucose metabolism according to the amount of glucose-6-phosphate produced. Guanosine behaved similarly, but glucose-6-phosphate was not formed from adenosine, deoxyinosine and deoxyguanosine.3. Glucose metabolism was related to the ATP content of the cells, which was raised by incubation with adenosine, or with inosine plus adenine.4. Adenosine and deoxyadenosine raised the potassium influx above the values found with inosine and deoxyinosine under conditions when the lactate production was constant. Active potassium influx was correlated with the ATP concentration in the cells, but independent of the lactate produ ction when the latter was raised above normal.

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The Accumulation of Divalent Ions by Isolated Mitochondria

Cited by 80 publications

References 9 publications

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Effect of Active Accumulation of Calcium and Phosphate Ions on the Structure of Rat Liver Mitochondria

Adenine Nucleotide-Induced Contraction of the Inner Mitochondrial Membrane

Potassium transport and nucleoside metabolism in human red cells

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