The Accumulation of Docosahexaenoic Acid in Rotifers by Feeding the DHA-enriched Yeast, Pichia methanolica HA-32

Aoki, Hideyuki; Furuya, Y.; Kono, Michiko; Furukawa, Kiyoshi; Endo, Yasushi; Fujimoto, Kenshiro

doi:10.5650/jos.53.461

Cited by 11 publications

(19 citation statements)

References 7 publications

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“…TAGs, fatty acid ethyl-esters or free-fatty acids) used as glucose cosubstrates upon the biochemical behavior of the yeast P. methanolica, and it was revealed that the intra-cellular lipid compounds were almost of the same chemical structure with the fatty material that was added into the medium. It was also interesting to state that in the case of the addition of free-fatty acids, fat accumulation ten-fold increased compared with the addition of methyl-esters or TAGs (lipid accumulation around 20% w/w, in dry matter against 2.5-2.8% w/w, respectively), while in the case of added free-fatty acids as co-substrate, SCO produced contained around 93% w/w, free-fatty acids into the total microbial lipids stored [123]. Growth of Geotrichum sp.…”

Section: Substrates Usedmentioning

confidence: 96%

“…Yeast lipid containing TAGs but also considerable quantities of free-fatty acids has been reported during growth of C. lipolytica 1094 on corn oil utilized as the sole substrate [33]. Likewise, Aoki et al [123] have studied the effect of the addition of various hydrophobic materials (e.g. TAGs, fatty acid ethyl-esters or free-fatty acids) used as glucose cosubstrates upon the biochemical behavior of the yeast P. methanolica, and it was revealed that the intra-cellular lipid compounds were almost of the same chemical structure with the fatty material that was added into the medium.…”

Section: Substrates Usedmentioning

confidence: 96%

“…However, in more recent investigations it has been demonstrated that some de novo biosynthesis of fatty acids from glucose or glycerol could potentially occur, in spite of the presence of significant exogenous quantities of fatty materials; for instance, the biochemical behavior of P. methanolica HA-32 was studied in media composed of glucose (at 50 g/L) and fish oil (or its derivatives ethyl-esters or free-fatty acids) (at 30 g/L). The fish oil (or its derivatives) contained significant quantities of docosahexanoic acid (DHA-D6, 9,12,15,17,19 C22:6), a fatty acid that through the de novo lipid accumulation from glucose can never be synthesized in P. methanolica [123]. Although the extra-cellular presence of TAGs or free-fatty acids into the culture medium resulted in an intra-cellular fatty acid profile similar to that of the substrate fat, and, therefore, significant DHA quantities were detected into the SCO produced presumably through its direct incorporation from the fatty substrate, the presence of fatty acid ethylesters resulted in the synthesis of a cellular lipid containing very restricted quantities of DHA, and presenting similarities with the one that had been synthesized when glucose was used as the sole carbon source [123].…”

Section: The Biochemistry Of Ex Novo Lipid Accumulationmentioning

confidence: 99%

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Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

593

496

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In the first part of this review, the biochemistry of lipid accumulation in the oleaginous microorganisms is depicted. Lipid biosynthesis form sugars and related substrates is a secondary anabolic activity, conducted after essential nutrient (usually nitrogen) depletion in the medium. Due to this exhaustion, the carbon flow is directed towards the accumulation of intracellular citric acid that is used as acetyl‐CoA donor in the cytoplasm. Acetyl‐CoA generates cellular fatty acids and subsequently triacylglycerols. Lipid accumulation from hydrophobic substrates is a growth associated process, being independent from nitrogen exhaustion in the medium. Medium fatty acids are incorporated with various incorporation rates and are either dissimilated for growth needs or become “substrate” for intracellular biotransformations. “New” fatty acid profiles (in both extra‐ and intracellular lipids) that did not previously exist in the medium are likely to be produced. Oleaginous microorganisms consume their own storage lipids when their metabolic abilities cannot be saturated by the extracellular carbon source. Reserve lipid breakdown is independent from the type of the carbon source used for lipid accumulation. In most cases it is accompanied by lipid‐free biomass production. Lipid mobilization is a specific process, as preferential degradation of the neutral lipid fractions is observed.

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Section: Substrates Usedmentioning

confidence: 96%

Section: Substrates Usedmentioning

confidence: 96%

Section: The Biochemistry Of Ex Novo Lipid Accumulationmentioning

confidence: 99%

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Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

593

496

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“…Since the various fatty acid substrates are removed at different rates from the medium and incorporated into the cell, the fatty acid composition of the fat medium can be altered as a function of fermentation time [6][7][8]. Incorporated fatty acids are either degraded for growth needs or used as substrate for intracellular biotransformations.…”

Section: Introductionmentioning

confidence: 99%

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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Yarrowia lipolytica ACA-DC 50109, maintained on potato dextrose agar (PDA, Fluka, Hannover, Germany) at T = 4 °C, was used. The salt composition of the culture medium was (g/l): KH 2 PO 4 7, Na 2 HPO 4 2. Selective uptake of fatty acids by the yeast Yarrowia lipolyticaYarrowia lipolytica, when cultivated on mixtures of free fatty acid substrates was found to remove C12:0, C14:0, ∆9 C18:1, ∆9,12 C18:2 and ∆9,12,15 C18:3 at significantly higher rates than C16:0 and C18:0, regardless of fatty acid composition of the initial substrate. C12:0, C14:0 and ∆9,12,15 C18:3 were specifically and completely removed from the substrate, while ∆9 C18:1 and ∆9,12 C18:2 concentrations decreased by 55-80 wt-% in comparison with initial concentrations. In contrast, concentration of C18:0 increased 2.1-3.5 fold in the substrate. Although C18:0 was removed slowly from the substrate, this fatty acid was selectively accumulated in the storage lipid. Inversely, only low quantities of ∆9 C18:1 and ∆9,12 C18:2 and traces of C12:0, C14:0 and ∆9,12,15 C18:3 were accumulated in the storage lipid. During storage lipid breakdown, cellular C16:0 and ∆9 C18:1 were taken up more rapidly than C18:0. We concluded that the capability of Yarrowia lipolytica to selectively remove several fatty acids from the substrate and accumulate others could be used in modification of the composition of selected mixtures of fatty acids and probably of common fats, to produce "new" fats with a predetermined composition.

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“…In the stationary growth phase (after 3 days) yeast cells were harvested by centrifugation (5 000 r/ min for 10 min) [14]. Then, harvested yeasts were divided into two groups and stored at -20 °C .…”

Section: Activation and Enhancement Of The Bioavailability Of β-Glucamentioning

confidence: 99%