The Accuracy of Mean Neutrophil Volume Relative to Blood Culture for the Diagnosis of Sepsis: A Meta-analysis

Villanueva, Emilio Iii; Almirol, Bernadette Joy

doi:10.21141/pjp.2017.004

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…The accuracy of the use of MNV may vary depending on age group, the nationality of patients as well as the model of analyzer used. 15 Table 3 displays the sensitivity and specificity in various studies nationally and internationally.…”

Section: Discussionmentioning

confidence: 99%

Utility of VCS Parameters as a Cost-effective and Early Marker of Sepsis: A Hospital-based Study

Joseph,

Goyal,

Singhal

2023

Indian Journal of Critical Care Medicine

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A bstract Introduction Sepsis is a life-threatening condition. Nowadays, hospitals rely on laboratory parameters like CRP and procalcitonin to detect sepsis. There is a need to evaluate and validate more accurate and early predictors of sepsis in critically ill patients. We analyzed volume, conductivity, and scatter (VCS) parameters of leukocytes in sepsis patients and compared them with the control group with no illness. Materials and methods It was a prospective case-control study. A total of 80 patients were studied with 40 sepsis cases and 40 controls. Peripheral smear examination was done in all the cases. Other parameters, such as WBC count, neutrophil%, absolute neutrophil count (ANC), High-sensitive C-reactive protein (hs-CRP), procalcitonin, and blood cultures were analyzed. We took the data of the patients from medical records and correlated it with other tests. Complete blood picture reports were generated by the Beckman Coulter LH series (LH 750 and 780). VCS parameters for neutrophils, lymphocytes and monocytes were compared between both groups. The results were analyzed using SPSS software (16.0 version). Results The age group was 20–85 years with male predominance. The mean neutrophil volume (MNV) and mean monocyte volume (MMV) were higher in the sepsis group when compared with the control group. The mean neutrophil conductivity and scatter was lower in the sepsis group and comparatively higher in the control group, but it was insignificant. The mean neutrophil volume values were higher in the sepsis group even with low total leukocyte count (TLC) when the patient had sepsis. There is no change in the mean lymphocyte VCS parameter and mean monocyte conductivity, scatter in both the groups. Conclusion The mean neutrophil volume and MMV are found to be good sensitive markers in the prediction of sepsis. Even when TLC is on the lower side in patients with sepsis, these parameters predict sepsis accurately. This helps clinicians to assess sepsis in patients at an early stage and has an important practical implication. How to cite this article Goyal H, Singhal A, Joseph M. Utility of VCS Parameters as a Cost-effective and Early Marker of Sepsis: A Hospital-based Study. Indian J Crit Care Med 2023;27(9):647–650.

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Section: Discussionmentioning

confidence: 99%

Utility of VCS Parameters as a Cost-effective and Early Marker of Sepsis: A Hospital-based Study

Joseph,

Goyal,

Singhal

2023

Indian Journal of Critical Care Medicine

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“…Changes in neutrophil ion and water homeostasis, frequently measured by changes in cell size, have been of increasing interest as diagnostic and prognostic markers in sepsis and other systemic inflammation conditions. A meta-analysis of the Area Under the Receiver Operating Characteristic Curve (AUROC) reported that a mean neutrophil volume of 0.87 (0.83–0.89) was an indicator for sepsis [ 12 ]. In accordance, alterations in neutrophil cell size occurred prior to organ dysfunction after severe injuries [ 16 ].…”

Section: Sepsis-induced Neutrophil Dysfunction and Its Correlationmentioning

confidence: 99%

“…Such a scenario can occur during the systemic inflammatory response in sepsis [ 3 , 5 , 6 , 7 ]. Recently, this neutrophil dysfunction has been increasingly associated with alterations in cellular size and intracellular cytosolic pH (henceforth referred to as “intracellular pH”), making an involvement of an ion–water disbalance likely [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Ion and Water Transport in Neutrophil Granulocytes and Its Impairment during Sepsis

Messerer

Schmidt

Frick

et al. 2021

IJMS

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Neutrophil granulocytes are the vanguard of innate immunity in response to numerous pathogens. Their activity drives the clearance of microbe- and damage-associated molecular patterns, thereby contributing substantially to the resolution of inflammation. However, excessive stimulation during sepsis leads to cellular unresponsiveness, immunological dysfunction, bacterial expansion, and subsequent multiple organ dysfunction. During the short lifespan of neutrophils, they can become significantly activated by complement factors, cytokines, and other inflammatory mediators. Following stimulation, the cells respond with a defined (electro-)physiological pattern, including depolarization, calcium influx, and alkalization as well as with increased metabolic activity and polarization of the actin cytoskeleton. Activity of ion transport proteins and aquaporins is critical for multiple cellular functions of innate immune cells, including chemotaxis, generation of reactive oxygen species, and phagocytosis of both pathogens and tissue debris. In this review, we first describe the ion transport proteins and aquaporins involved in the neutrophil ion–water fluxes in response to chemoattractants. We then relate ion and water flux to cellular functions with a focus on danger sensing, chemotaxis, phagocytosis, and oxidative burst and approach the role of altered ion transport protein expression and activity in impaired cellular functions and cell death during systemic inflammation as in sepsis.

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Role of Automated Volume, Conductivity and Scatter (VCS) Parameters of Neutrophils as Indicators of Sepsis

Vaswani

Kakkar

Kwatra³

et al. 2022

Indian J Hematol Blood Transfus

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The Accuracy of Mean Neutrophil Volume Relative to Blood Culture for the Diagnosis of Sepsis: A Meta-analysis

Cited by 5 publications

References 14 publications

Utility of VCS Parameters as a Cost-effective and Early Marker of Sepsis: A Hospital-based Study

Utility of VCS Parameters as a Cost-effective and Early Marker of Sepsis: A Hospital-based Study

Ion and Water Transport in Neutrophil Granulocytes and Its Impairment during Sepsis

Role of Automated Volume, Conductivity and Scatter (VCS) Parameters of Neutrophils as Indicators of Sepsis

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