The Accuracy of Serologic Diagnosis of <i>Helicobacter pylori</i> Infection in School‐aged Children of Mixed Ethnicity

Tindberg, Ylva; Bengtsson, Carina; Bergström, Mats; Granström, Marta

doi:10.1046/j.1523-5378.2001.00005.x

Cited by 24 publications

(30 citation statements)

References 33 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, no previous study has systematically compared antibody levels in individuals from the same population with different manifestations of H. pylori infection. We have previously noted that when using ELISA for screening of Swedish school children at 10 years of age, our cutoff level previously established in culture-confirmed patients within a Swedish population would have resulted in a loss of sensitivity (15). We speculated that the need to lower the cutoff level might have been due to the fact that the children were 10 years of age, while the patients were adults.…”

Section: Discussionmentioning

confidence: 98%

“…It would also explain our previous observation that the cutoff level established for symptomatic patients needed to be lowered for screening purposes for 10-year-old children (15). The problem is of general relevance since cutoff levels in serological assays are established against a "gold standard", usually culture.…”

Section: Discussionmentioning

confidence: 99%

“…The upper limit of normal values, at an optical density of 0.36 (A 405 ) for the Swedish antigen, had been established in 83 culture-positive adult patients and 43 patients negative by microscopy, culture, rapid urease test, giving a sensitivity of 100% (83 of 83) and specificity of 95.6% (43 of 45) (2,14,15).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously developed and evaluated an in-house ELISA based on sonicated H. pylori antigen, supplemented with an absorption step with sonicated Campylobacter jejuni antigen to remove cross-reacting antibodies (2,14,15). In order to provide serodiagnostic and seroepidemiological tools for H. pylori infection in Vietnam, the present evaluation of the in-house ELISA was initiated in the local population, both in patients with peptic ulcer disease where the infection had been confirmed by culture of H. pylori and in a sample of the general population where immunoblot could be used as reference method.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Enzyme-Linked Immunosorbent Assay forHelicobacter pyloriNeeds Adjustment for the Population Investigated

et al. 2004

Self Cite

View full text Add to dashboard Cite

Helicobacter pylori infection and peptic ulcer disease are common in developing countries, e.g., Vietnam. An enzyme-linked immunosorbent assay (ELISA) for screening of patients and for seroepidemiology is a useful tool but needs to be validated in the population studied. We used in-house ELISA with sonicated Swedish and Vietnamese strains as antigens to measure immunoglobulin G antibodies after absorption with sonicated Campylobacter jejuni in sera from 270 H. pylori culture-confirmed peptic ulcer patients, 128 Swedish ureabreath test and immunoblot-positive healthy controls, and 432 Vietnamese immunoblot-positive population controls. Sonicated whole-cell antigen based on the local strains showed a significantly better performance. Immunoblot-positive peptic ulcer patients had significantly higher antibody concentrations than immunoblotpositive population controls, necessitating a lower cutoff level if serology is used for screening or epidemiological purposes. The study shows that the parameters of ELISA for H. pylori need to be adjusted for the population being investigated.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Enzyme-Linked Immunosorbent Assay forHelicobacter pyloriNeeds Adjustment for the Population Investigated

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…Information about family characteristics was obtained by a questionnaire completed by the parents. Blood was drawn to determine serologically the H. pylori infection status with an in-house enzyme-linked immunosorbent assay and immunoblot (Helico Blot 2.0, Genelabs Diagnostics, Singapore) (34). H. pylori-seropositive individuals were invited to undergo gastroscopy with culture of biopsy samples from the corpus and antrum.…”

Section: Methodsmentioning

confidence: 99%

Concordance ofHelicobacter pyloriStrains within Families

et al. 2003

Self Cite

View full text Add to dashboard Cite

Helicobacter pylori infection is typically acquired in early childhood, and a predominantly intrafamilial transmission has been postulated. To what extent family members share the same strains is poorly documented. Our aim was to explore patterns of shared strains within families by using molecular typing. Family members of H. pylori-infected 10-to 12-year-old index children identified in a school survey were invited to undergo gastroscopy. Bacterial isolates were typed with random amplified polymorphic DNA and PCRrestriction fragment length polymorphism of the genes ureA-B, glmM, or flaA. The presence or absence of the cag pathogenicity island, a bacterial virulence factor, was determined by PCR. GelCompar II software, supplemented with visual inspection, was used in the cluster analysis. In 39 families, 104 individuals contributed 208 bacterial isolates from the antrum and corpus. A large proportion, 29 of 36 (81%) of the offspring in a sibship, harbored the same strain as at least one sibling. Mother-offspring strain concordance was detected in 10 of 18 (56%) of the families. Of 17 investigated father-offspring relations in eight families, none were strain concordant. Spouses were infected with the same strains in 5 of 23 (22%) of the couples. Different strains in the antrum and corpus were found in 8 of 104 (8%) of the subjects. Our family-based fingerprinting study demonstrates a high proportion of shared strains among siblings. Transmission between spouses seems to be appreciable. The data support mother-child and sib-sib transmission as the primary transmission pathways of H. pylori.Helicobacter pylori colonizes half of the world's population, and the infection is associated with chronic gastritis, peptic ulcer, gastric cancer, and gastric lymphoma. In-depth knowledge of the transmission patterns may constitute important information for future intervention strategies.In the absence of consistent and verified environmental reservoirs, a predominantly person-to-person transmission has been postulated. H. pylori infection is associated with poor living conditions, and possible transmission routes are fecaloral, oral-oral, or gastro-oral, but firm evidence is lacking (36). The infection clusters in families and is usually acquired in early childhood. A child's risk of being infected is largely determined by the presence or absence of infected family members. Having an infected mother has been suggested to be a more prominent risk factor than having an infected father (30,35). Indications of sib-sib transmission have been reported (14). Transmission between spouses occurs but its significance is unclear (5, 9, 19).Molecular typing of pathogens can corroborate and further characterize the transmission pathways suggested by epidemiologic data based on infection status. Shared strains among individuals indicate person-to-person transmission or acquisition from a common source. Unrelated individuals harbor distinct H. pylori isolates (1), while clonal lineages can be discerned within families (2,7,11,16,20,22,24,2...

show abstract

Non-invasive diagnostic tests forHelicobacter pyloriinfection

Best

Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

157

130

View full text Add to dashboard Cite

In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

show abstract

The Accuracy of Serologic Diagnosis of Helicobacter pylori Infection in School‐aged Children of Mixed Ethnicity

Cited by 24 publications

References 33 publications

Enzyme-Linked Immunosorbent Assay forHelicobacter pyloriNeeds Adjustment for the Population Investigated

Enzyme-Linked Immunosorbent Assay forHelicobacter pyloriNeeds Adjustment for the Population Investigated

Concordance ofHelicobacter pyloriStrains within Families

Non-invasive diagnostic tests forHelicobacter pyloriinfection

Contact Info

Product

Resources

About