2002
DOI: 10.1002/yea.936
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The acetyl co‐enzyme A synthetase genes of Kluyveromyces lactis

Abstract: Two Kluyveromyces lactis genes encoding acetyl co-enzyme A synthetase isoenzymes were isolated. One we named KlACS1, as it has high similarity to the ACS1 gene of Saccharomyces cerevisiae. The other gene, KlACS2, showed more similarity to S. cerevisiae ACS2 than to KlACS1 or ScACS1. This suggests that divergence of the two isogenes occurred before the evolutionary separation of the species and that the different functions have been conserved. In line with this idea is the regulation of transcription of the gen… Show more

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Cited by 24 publications
(21 citation statements)
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“…Wild-type and Klsdh1⌬ mutant strains were grown overnight on YP medium containing 1% glucose or 2% lactate, and total RNAs were prepared from these cultures. In K. lactis the regulation of the two genes for acetyl-CoA synthetase has been previously described: KlACS1 is expressed at low level on glucose or ethanol and induced on acetate or lactate, while KlACS2 is preferentially expressed on glucose and ethanol (30,55). Such regulation of the ACS genes is present also in our wild-type strain grown on glucose (Fig.…”
Section: Resultssupporting
confidence: 67%
See 1 more Smart Citation
“…Wild-type and Klsdh1⌬ mutant strains were grown overnight on YP medium containing 1% glucose or 2% lactate, and total RNAs were prepared from these cultures. In K. lactis the regulation of the two genes for acetyl-CoA synthetase has been previously described: KlACS1 is expressed at low level on glucose or ethanol and induced on acetate or lactate, while KlACS2 is preferentially expressed on glucose and ethanol (30,55). Such regulation of the ACS genes is present also in our wild-type strain grown on glucose (Fig.…”
Section: Resultssupporting
confidence: 67%
“…We performed a Northern analysis of the genes involved in pyruvate utilization to look at the metabolic route of its dissimilation. Therefore, we analyzed the expression levels of the genes for pyruvate decarboxylase (KlPDCA) (6) and acetylcoenzyme A (acetyl-CoA) synthetase (KlACS1 and KlACS2) (55), and two genes of the glyoxylate cycle, namely isocitrate lyase (KlICL1) (32) and malate synthase (KlMLS1) (see Fig. 8 for a representation of the reactions catalyzed by these activities).…”
Section: Resultsmentioning
confidence: 99%
“…This condition may be more evident under unfavorable conditions, such as cellular recovery from electroporation and growth on solid media with poor nutrition. For instance, the acs2 mutant of K. lactis requires three weeks to form large colonies [27]. Therefore, the possibility that the actual gene targeting efficiency can be reduced is high because of the unformed colonies.…”
Section: Discussionmentioning
confidence: 99%
“…Like yeast (455,487), mammals possess two isoforms of AMP-ACS (144,281,475,480), one cytosolic and the other mitochondrial. The activity of the cytosolic enzyme (AceCS1), found predominantly in the liver, activates acetate to supply cells with acetyl-CoA for lipid synthesis (281,475).…”
Section: Mammalian "Acetate Switch"mentioning
confidence: 99%