The Acid-Tolerant Microbiota Associated with Plaque from Initial Caries and Healthy Tooth Surfaces

Svensäter, Gunnel; Borgström, Margareta Krasse; Bowden, G. H.; Edwardsson, S.

doi:10.1159/000073390

Cited by 70 publications

(67 citation statements)

References 27 publications

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“…Previous studies have shown that OPN binds to the cell surface of S. mitis [7], a pioneer colonizer of dental enamel, which is also associated with the development of caries lesions [37][38][39][40][41]. The presence of OPN hampers adhesion of S. mitis to saliva-coated surfaces and diminishes biofilm formation in a multi-species in vitro oral biofilm model dominated by S. mitis [8].…”

Section: Discussionmentioning

confidence: 99%

Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow

Kristensen

Zeng

Neu

et al. 2017

Journal of Oral Microbiology

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The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion to dental tissues and control biofilm formation. This study sought to determine to what extent OPN impacts adhesion forces and surface attachment of different bacterial strains involved in dental caries or medical device-related infections. It further investigated if OPN's effect on adhesion is caused by blocking the accessibility of glycoconjugates on bacterial surfaces. Bacterial adhesion was determined in a shear-controlled flow cell system in the presence of different concentrations of OPN, and interaction forces of single bacteria were quantified using single-cell force spectroscopy before and after OPN exposure. Moreover, the study investigated OPN's effect on the accessibility of cell surface glycoconjugates through fluorescence lectin-binding analysis. OPN strongly affected bacterial adhesion in a dose-dependent manner for all investigated species (Actinomyces naeslundii, Actinomyces viscosus, Lactobacillus paracasei subsp. paracasei, Staphylococcus epidermidis, Streptococcus mitis, and Streptococcus oralis). Likewise, adhesion forces decreased after OPN treatment. No effect of OPN on the lectin-accessibility to glycoconjugates was found. OPN reduces the adhesion and adhesion force/energy of a variety of bacteria and has a potential therapeutic use for biofilm control. OPN acts upon bacterial adhesion without blocking cell surface glycoconjugates. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow

Kristensen

Zeng

Neu

et al. 2017

Journal of Oral Microbiology

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“…S. mutans H7, the strain used in this study, was isolated from dental plaque above a carious enamel surface following plating directly onto a nonselective, solid agar medium buffered at pH 5.0 (33). For the labeling experiments, single colonies on blood agar were used to inoculate minimal medium containing 40 mM phosphate-citrate buffer (pH 7.5) and 20 mM glucose (MM4) (14).…”

Section: Methodsmentioning

confidence: 99%

Protein Expression by Streptococcus mutans during Initial Stage of Biofilm Formation

Welin

Wilkins

Beighton

et al. 2004

Appl Environ Microbiol

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Cells growing on surfaces in biofilms exhibit properties distinct from those of planktonic cells, such as increased resistance to biocides and antimicrobial agents. In spite of increased interest in biofilms, very little is known about alterations in cell physiology that occur upon attachment of cells to a surface. In this study we have investigated the changes induced in the protein synthesis by contact of Streptococcus mutans with a surface. Log-phase planktonic cells of S. mutans were allowed to adhere to a glass slide for 2 h in the presence of a 14 C-amino acid mixture. Nonadhered cells were washed away, and the adhered cells were removed by sonication. The proteins were extracted from the nonadhered planktonic and the adhered biofilm cells and separated by two-dimensional gel electrophoresis followed by autoradiography and image analysis. Image analysis revealed that the relative rate of synthesis of 25 proteins was enhanced and that of 8 proteins was diminished >1.3-fold in the biofilm cells. Proteins of interest were identified by mass spectrometry and computer-assisted protein sequence analysis. Of the 33 proteins associated with the adhesion response, all but 10 were identified by mass spectrometry and peptide mass fingerprinting. The most prominent change in adhered cells was the increase in relative synthesis of enzymes involved in carbohydrate catabolism indicating that a redirection in protein synthesis towards energy generation is an early response to contact with and adhesion to a surface.

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“…Clear evidence shows that cells in biofilms are in a physiological state that differs from their planktonic counterparts and tend to be less susceptible to antimicrobial agents [3]. The formation of acid end products through the metabolism of carbohydrates by acidogenic microorganisms within these biofilms is an important factor in the development of dental caries [4].…”

Section: Introductionmentioning

confidence: 99%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brainheart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L. acidophilus 10 8 CFU/mL and S. mutans 10 8 CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm 2 light emission diode. Four different groups were analyzed L−D− (control group), L−D+ (drug group), L+D− (light group), and L+D+ (PDT group). ANOVA/Tukey's tests were conducted to compare groups. A significant reduction (p <0.05) in cell viability was observed in the biofilm phase following photosensitization with all curcumin concentrations tested. To achieve significant bacterial reduction (p <0.05) in carious dentine, it was necessary to utilize 5.0 g/L of curcumin in association with blue light. No significant reduction was found for L−D+, supporting the absence of the drug's dark toxicity. S. mutans and L. acidophilus were susceptible to curcumin in the presence of blue light. However, due to light penetration and drug diffusion difficulties, these microorganisms within dentine carious lesions were less affected than they were in the biofilm phase.

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The Acid-Tolerant Microbiota Associated with Plaque from Initial Caries and Healthy Tooth Surfaces

Cited by 70 publications

References 27 publications

Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow

Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow

Protein Expression by Streptococcus mutans during Initial Stage of Biofilm Formation

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

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