The Acrosomal Region and the Acrosome Reaction in Sperm of the Golden Hamster1

Franklin, L. E.; Barros, C.; Fussell, E. N.

doi:10.1093/biolreprod/3.2.180

Cited by 102 publications

(47 citation statements)

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“…The dense acrosomal granule has been well described in early electron microscopy studies of the development of the acrosome in mammalian sperm (Burgos and Fawcett, 1955;Franklin et al, 1970;Susi et al, 1971;Fawcett, 1975;Griffiths et al, 1980). This study has not determined how long proacrosin is secreted by the Golgi.…”

Section: Discussionmentioning

confidence: 91%

Localization of boar sperm proacrosin during spermatogenesis and during sperm maturation in the epididymis

et al. 1991

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The localization of proacrosin was determined by using colloidal gold labeling and electron microscopy of boar germ cells during spermiogenesis to post-ejaculation. Proacrosin was first localized in round spermatids during the Golgi phase of spermiogenesis; it was associated with the electron-dense granule, or acrosomal granule that was conspicuous within the acrosome. It remained within the acrosomal granule during the cap and acrosome phases of spermiogenesis. At these stages, there was no apparent association of the proacrosin molecule with the acrosomal membranes. During the maturation phase of spermiogenesis, proacrosin was seen to become dispersed into all regions of the acrosome except the equatorial segment. When sperm from different segments of the epididymis and ejaculated sperm were examined, localization was observed throughout the acrosome except for the equatorial segment. Here proacrosin appeared to be localized on both the inner and outer acrosomal membranes as well as with the acrosomal matrix, although further studies are required to verify the membrane localization. No labeling was seen on the plasma membrane. These data suggest that the synthesis and movement of proacrosin to sites in the acrosome are controlled by an as yet unknown process. The absence of proacrosin on the plasma membrane of mature ejaculated sperm makes it unlikely that this enzyme plays a role in sperm-zona adhesion prior to capacitation.

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Section: Discussionmentioning

confidence: 91%

Localization of boar sperm proacrosin during spermatogenesis and during sperm maturation in the epididymis

et al. 1991

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“…In contrast to the false acrosome reaction of deteriorating spermatozoa, Yanagimachi & Usui (1974) and Green (1978) indicated that there was a general swelling of the anterior acrosome of the guinea-pig before the true acrosome reaction. Yanagimachi & Noda (1970) (Franklin, Barros & Fussel, 1970; (Mann, 1964). However, the specific cause of SAR is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Morphological change of the acrosome on motile bovine spermatozoa due to storage at 4 C

Aalseth¹,

Saacke²

1985

Reproduction

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Summary. Swelling of the apical ridge and anterior acrosome of motile bovine spermatozoa was observed during in-vitro storage using differential interference\x=req-\ contrast optics. This morphological alteration is different from that described as the false acrosome reaction on immotile spermatozoa, apparent in ageing semen samples and which has been associated with cell death. In this study, transmission electron microscopy revealed that the apical ridge acrosomal matrix was extended into complex folds and/or projections. Acrosomal and plasma membrane integrity was retained. Storing spermatozoa (1500 \m=x\ 106/ml) in seminal plasma at 4\s=deg\Cfor 1 day was most conducive to the swelling of the apical ridge. Replacing seminal plasma with egg yol k\p=n-\ citrate inhibited swelling. However, incubating semen at 37\s=deg\Cin egg yol k\p=n-\Tri s\p=n-\ fructose extender (25 \m=x\106 spermatozoa/ml) after storage in egg yolk\p=n-\citrate at 4\s=deg\Cfor \ m=ge\ 3 days restored the swelling characteristic.

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“…The equatorial segment of the head was delineated by topographical relief at its margins. The anterior margin is the ridgeof thickened perinuclearmaterial (part of the theca) and the posterior margin being the site of reflection of the acrosomal membrane (Franklin, Barros & Fussell, 1970). Golden hamster spermatozoashowedno variation in topographyof the intact acrosomalcap region (PI.…”

Section: Hamstermentioning

confidence: 99%

Photoelectron imaging of guinea-pig, hamster and human spermatozoa

Mrsny¹,

Griffith²

1985

Reproduction

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Summary. Photoelectron images of mammalian spermatozoa were obtained by subjecting the specimens to u.v.-irradiation and focussing the emitted electrons by electron optics (photoelectron microscopy). Guinea-pig, hamster and human sperm atozoa were fixed in glutaraldehyde, deposited on conductive glass discs, and dehydrated. Sufficient quantities of photoelectrons were released from the surface of spermatozoa to produce images without staining, coating or metal shadowing. The large planar heads of guinea-pig spermatozoa were easily resolved with good delineation of acrosomal and postacrosomal regions. Residual vesicles could be visualized on the surface of the inner acrosomal membrane of spermatozoa that had undergone the acrosome reaction. Also detectable in these photoelectron images were finer membrane surface details, periodicities in the midpiece region of the tail which coincided with the distribution of mitochondria, and periodicities in the principal piece which appeared to be related to fibrous sheath components. Hamster spermatozoa were similarly well resolved but human spermatozoa were more difficult to image because of their increased surface curvature. The mechanism responsible for detection of these surface details is primarily topographical contrast rather than material contrast, since spermatozoa coated with a thin layer of gold or platinum exhibited similar features, althoughat reduced resolution, as the uncoated specimens.

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The Acrosomal Region and the Acrosome Reaction in Sperm of the Golden Hamster1

Cited by 102 publications

References 0 publications

Localization of boar sperm proacrosin during spermatogenesis and during sperm maturation in the epididymis

Localization of boar sperm proacrosin during spermatogenesis and during sperm maturation in the epididymis

Morphological change of the acrosome on motile bovine spermatozoa due to storage at 4 C

Photoelectron imaging of guinea-pig, hamster and human spermatozoa

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