The actin binding cytoskeletal protein Moesin is involved in nuclear mRNA export

Kristó, Ildikó; Bajusz, Csaba; Borsos, Barbara N.; Pankotai, Tibor; Dopie, Joseph; Jankovics, Ferenc; Vartiainen, Maria K.; Erdélyi, Miklós; Vilmos, Péter

doi:10.1016/j.bbamcr.2017.05.020

Cited by 13 publications

(25 citation statements)

References 52 publications

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“…Moe has been shown previously to localize to the heat‐shock puffs at cytological locations 87A and 87C [16]. To further confirm that Moe is responsible for the regulation of heat‐shock gene transcription, we tested whether WT Moe localizes to the actively transcribing heat‐shock genes identified in the transcriptome experiment.…”

Section: Resultsmentioning

confidence: 94%

“…In our previous work, we discovered that upon heat shock the amount of Moe significantly increases in the nucleus [16]. Therefore, we tested if the NES-tagged Moe isoform can also localize to the nucleus at high levels, by ectopically expressing GFP-labeled Moe and Moe NES proteins in the larval salivary gland cells (Fig.…”

Section: Transcriptome Analysis Reveals Altered Gene Expression In Moe Nes Mutantsmentioning

confidence: 99%

“…Similarly to their major binding partner, actin and other cytoskeletal proteins [1], ERMs have been found to localize to the cell nucleus in numerous species [12][13][14][15]. In Drosophila melanogaster, the sole ERM representative, Moesin (Moe), has been shown to be a functional component of the nucleus by participating in mRNA export [16]. However, the in vivo importance of the nuclear localization and activity of ERMs is not known at present.…”

Section: Introductionmentioning

confidence: 99%

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The nuclear activity of the actin‐binding Moesin protein is necessary for gene expression in Drosophila

et al. 2021

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Ezrin-Radixin-Moesin (ERM) proteins play an essential role in the cytoplasm by cross-linking actin filaments with plasma membrane proteins. Research has identified the nuclear localization of ERMs, as well as the involvement of a single Drosophila ERM protein, Moesin, in nuclear mRNA exports. However, the question of how important the nuclear activity of ERM proteins are for the life of an organism has so far not been explored. Here, we present the first attempt to reveal the in vivo relevance of nuclear localization of Moesin in Drosophila. With the help of a nuclear export signal, we decreased the amount of Moesin in the nuclei of the animals. Furthermore, we observed various developmental defects, demonstrating the importance of ERM function in the nucleus for the first time. Transcriptome analysis of the mutant flies revealed that the lack of nuclear Moesin function leads to expression changes in nearly 700 genes, among them heat-shock genes. This result together with additional findings revealed that in Drosophila the expression of protein chaperones requires the nuclear functions of Moesin.

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Section: Resultsmentioning

confidence: 94%

Section: Transcriptome Analysis Reveals Altered Gene Expression In Moe Nes Mutantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The nuclear activity of the actin‐binding Moesin protein is necessary for gene expression in Drosophila

et al. 2021

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“…To investigate whether loss of nup62, emb, nup214, nup88 or profilin function affects mRNA export in cyst cell nuclei, we assayed for the nuclear vs. cytoplasmic distribution of the mRNA export factor Rae1, by expressing a UAS-Rae1-GFP transgene. Rae1 is a shuttling nucleoporin, member of the WD40 protein family, and carrier of mRNA out of the nucleus (Kristo et al, 2017;Sitterlin, 2004). Knockdown of nup62, emb, nup214, nup88 or profilin in cyst cells led to higher Rae1 levels in cyst cell nuclei and strong reduction of Rae1 in the cytoplasm of cyst cells ( Fig.…”

Section: Suppression Of Apoptosis In Cyst Cells Can Partially Restorementioning

confidence: 98%

Nuclear export in somatic cyst cells controls cyst cell-germline coordination and germline differentiation in theDrosophilatestis

Papagiannouli

Fuller

Lohmann

2018

Preprint

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Nucleocytoplasmic communication is crucial for proper cell function and coordination of intrinsic cues with signaling responses emanating from the neighboring cells and the local tissue microenvironment. In the Drosophila male germline system, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small cyst, the functional unit of differentiation. Here we show that the peripheral nucleoporins Nup62, Nup214 and Nup88, and the exportin Emb are critically required in cyst cells to maintain cyst cell survival and germline encapsulation in order to protect cyst cell-germline communication and promote germ cell differentiation. Knockdown of nup62, emb, nup214 or nup88 in cyst cells leads to cell-autonomous defects in mRNA export, and cell non-autonomous overproliferation of early germ cells in the absence of cyst cell-derived differentiation signals. Suppression of apoptosis can reverse cyst cell elimination and partially restored those defects. Interestingly, overexpression of the Drosophila Profilin gene chickadee can rescue cyst cell survival and restore germline encapsulation and differentiation, by counteracting Ntf-2 mediated export, suggesting that the function of Profilin in cyst cells is linked to nuclear export.

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“…Moe is the single fly ortholog of Ezrin-Radixin-Moesin (ERM) proteins that link the apical membrane to the cortical actin cytoskeleton (Solinet et al, 2013). There is some evidence that Moe may also regulate mRNA export from the nucleus (Kristó et al, 2017). More notably, Moe interacts directly with other proteins encoded by mRNAs localized apically in cellularized embryos, including Crb and Patj (Médina et al, 2002).…”

Section: Many Localized Mrnas Encode Cell Cortex and Junction Proteinsmentioning

confidence: 99%

Drosophila mRNA Localization During Later Development: Past, Present, and Future

Hughes

Simmonds

2019

Front. Genet.

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Multiple mechanisms tightly regulate mRNAs during their transcription, translation, and degradation. Of these, the physical localization of mRNAs to specific cytoplasmic regions is relatively easy to detect; however, linking localization to functional regulatory roles has been more difficult to establish. Historically, Drosophila melanogaster is a highly effective model to identify localized mRNAs and has helped identify roles for this process by regulating various cell activities. The majority of the well-characterized functional roles for localizing mRNAs to sub-regions of the cytoplasm have come from the Drosophila oocyte and early syncytial embryo. At present, relatively few functional roles have been established for mRNA localization within the relatively smaller, differentiated somatic cell lineages characteristic of later development, beginning with the cellular blastoderm, and the multiple cell lineages that make up the gastrulating embryo, larva, and adult. This review is divided into three parts—the first outlines past evidence for cytoplasmic mRNA localization affecting aspects of cellular activity post-blastoderm development in Drosophila. The majority of these known examples come from highly polarized cell lineages such as differentiating neurons. The second part considers the present state of affairs where we now know that many, if not most mRNAs are localized to discrete cytoplasmic regions in one or more somatic cell lineages of cellularized embryos, larvae or adults. Assuming that the phenomenon of cytoplasmic mRNA localization represents an underlying functional activity, and correlation with the encoded proteins suggests that mRNA localization is involved in far more than neuronal differentiation. Thus, it seems highly likely that past-identified examples represent only a small fraction of localization-based mRNA regulation in somatic cells. The last part highlights recent technological advances that now provide an opportunity for probing the role of mRNA localization in Drosophila, moving beyond cataloging the diversity of localized mRNAs to a similar understanding of how localization affects mRNA activity.

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The actin binding cytoskeletal protein Moesin is involved in nuclear mRNA export

Cited by 13 publications

References 52 publications

The nuclear activity of the actin‐binding Moesin protein is necessary for gene expression in Drosophila

The nuclear activity of the actin‐binding Moesin protein is necessary for gene expression in Drosophila

Nuclear export in somatic cyst cells controls cyst cell-germline coordination and germline differentiation in theDrosophilatestis

Drosophila mRNA Localization During Later Development: Past, Present, and Future

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